The development of an industrial process to produce AC γ-linolenic acid using Choanephora cucurbitarum

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dc.contributor.advisorWoods, David Ren_ZA
dc.contributor.authorZiniades, Catherineen_ZA
dc.date.accessioned2016-10-03T04:06:55Z
dc.date.available2016-10-03T04:06:55Z
dc.date.issued1990en_ZA
dc.descriptionBibliography: pages 163-169.en_ZA
dc.description.abstractThe objective of this work was to produce γ-linolenic acid (γLA) using a fungus in submerged fermentation. Selection work was aimed at identifying a fungal strain capable of yielding a high level of γLA in an industrial fermentation. Thirty-nine fungal strains were screened under shake flask conditions. The major criteria used in evaluating these strains were, the yield of γLA per unit volume (g/l) and γLA as a percentage of fatty acids, which is important in the downstream processing of γLA . Other parameters of industrial importance such as strain handling and the fatty acid profile were also considered. Eleven fungi in the order Phycomycetes were identified after initial screening. From these fungi, a strain of Choanephora cucurbitarum was found to give superior γLA yields. c. cucurbitarum produced γLA yields of 331mg/l and 674mg/l in shake flask and laboratory fermenters respectively. This strain had other industrially beneficial qualities such as good sporulation, a good biomass of 22, 5g/l and a relatively high yield of γLA of 2,99g/100g dry matter. Subsequently a Zygorhynchus heterogamus strain was found to give similar yields of γLA to c. cucurbitarum. z. heterogamus also had a high γLA : linoleic acid ratio which aids the purification of γLA . This is the first known report of a high level of γLA in the genus Zygorhynchus. The industrial development of γLA production by Zygorhynchus is not reported.en_ZA
dc.identifier.apacitationZiniades, C. (1990). <i>The development of an industrial process to produce AC γ-linolenic acid using Choanephora cucurbitarum</i>. (Thesis). University of Cape Town ,Faculty of Science ,Department of Molecular and Cell Biology. Retrieved from http://hdl.handle.net/11427/22047en_ZA
dc.identifier.chicagocitationZiniades, Catherine. <i>"The development of an industrial process to produce AC γ-linolenic acid using Choanephora cucurbitarum."</i> Thesis., University of Cape Town ,Faculty of Science ,Department of Molecular and Cell Biology, 1990. http://hdl.handle.net/11427/22047en_ZA
dc.identifier.citationZiniades, C. 1990. The development of an industrial process to produce AC γ-linolenic acid using Choanephora cucurbitarum. University of Cape Town.en_ZA
dc.identifier.ris TY - Thesis / Dissertation AU - Ziniades, Catherine AB - The objective of this work was to produce γ-linolenic acid (γLA) using a fungus in submerged fermentation. Selection work was aimed at identifying a fungal strain capable of yielding a high level of γLA in an industrial fermentation. Thirty-nine fungal strains were screened under shake flask conditions. The major criteria used in evaluating these strains were, the yield of γLA per unit volume (g/l) and γLA as a percentage of fatty acids, which is important in the downstream processing of γLA . Other parameters of industrial importance such as strain handling and the fatty acid profile were also considered. Eleven fungi in the order Phycomycetes were identified after initial screening. From these fungi, a strain of Choanephora cucurbitarum was found to give superior γLA yields. c. cucurbitarum produced γLA yields of 331mg/l and 674mg/l in shake flask and laboratory fermenters respectively. This strain had other industrially beneficial qualities such as good sporulation, a good biomass of 22, 5g/l and a relatively high yield of γLA of 2,99g/100g dry matter. Subsequently a Zygorhynchus heterogamus strain was found to give similar yields of γLA to c. cucurbitarum. z. heterogamus also had a high γLA : linoleic acid ratio which aids the purification of γLA . This is the first known report of a high level of γLA in the genus Zygorhynchus. The industrial development of γLA production by Zygorhynchus is not reported. DA - 1990 DB - OpenUCT DP - University of Cape Town LK - https://open.uct.ac.za PB - University of Cape Town PY - 1990 T1 - The development of an industrial process to produce AC γ-linolenic acid using Choanephora cucurbitarum TI - The development of an industrial process to produce AC γ-linolenic acid using Choanephora cucurbitarum UR - http://hdl.handle.net/11427/22047 ER - en_ZA
dc.identifier.urihttp://hdl.handle.net/11427/22047
dc.identifier.vancouvercitationZiniades C. The development of an industrial process to produce AC γ-linolenic acid using Choanephora cucurbitarum. [Thesis]. University of Cape Town ,Faculty of Science ,Department of Molecular and Cell Biology, 1990 [cited yyyy month dd]. Available from: http://hdl.handle.net/11427/22047en_ZA
dc.language.isoengen_ZA
dc.publisher.departmentDepartment of Molecular and Cell Biologyen_ZA
dc.publisher.facultyFaculty of Scienceen_ZA
dc.publisher.institutionUniversity of Cape Town
dc.subject.otherOils and fats - Microbiologyen_ZA
dc.titleThe development of an industrial process to produce AC γ-linolenic acid using Choanephora cucurbitarumen_ZA
dc.typeMaster Thesis
dc.type.qualificationlevelMasters
dc.type.qualificationnameMScen_ZA
uct.type.filetypeText
uct.type.filetypeImage
uct.type.publicationResearchen_ZA
uct.type.resourceThesisen_ZA
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