Molecular characterisation of the fibre-digesting bacteria isolated from the ostrich (Struthio Camelus var. Domesticus) hindgut

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dc.contributor.advisorThomson, Jennifer Annen_ZA
dc.contributor.advisorReid, Sharon Jen_ZA
dc.contributor.authorRussouw, Tracy Karen Joeen_ZA
dc.date.accessioned2014-11-20T03:57:34Z
dc.date.available2014-11-20T03:57:34Z
dc.date.issued2000en_ZA
dc.descriptionIncludes bibliographical references.en_ZA
dc.description.abstractEleven bacterial strains that have been isolated from the ostrich hindgut, have successfully been identified with the aid of PCR amplification of the 16S rDNA genes and sequence analysis using the BLASTN search of the GenBank database. The nucleotide sequences were determined either through direct sequencing of the PCR products or after cloning the products into the plasmid vector pBluescript (SK). The results obtained from the 16S rDNA sequence homologies strongly suggests that the newly identified bacteria should be assigned to three major bacterial genera, namely, Ruminococcus, Buryrivibrio and Bacteroides. Four highly cellulolytic strains were identified as ruminococci while two weakly cellulolytic strains, showed highest homology to members of the genus Butyrivibrio. Another weakly cellulolytic strain showed closest identity to the sulfur reducing bacterial genus Desulfovibrio. Four non-cellulolytic strains showed highest homology to the genus Bacteroides.en_ZA
dc.identifier.apacitationRussouw, T. K. J. (2000). <i>Molecular characterisation of the fibre-digesting bacteria isolated from the ostrich (Struthio Camelus var. Domesticus) hindgut</i>. (Thesis). University of Cape Town ,Faculty of Science ,Department of Molecular and Cell Biology. Retrieved from http://hdl.handle.net/11427/9740en_ZA
dc.identifier.chicagocitationRussouw, Tracy Karen Joe. <i>"Molecular characterisation of the fibre-digesting bacteria isolated from the ostrich (Struthio Camelus var. Domesticus) hindgut."</i> Thesis., University of Cape Town ,Faculty of Science ,Department of Molecular and Cell Biology, 2000. http://hdl.handle.net/11427/9740en_ZA
dc.identifier.citationRussouw, T. 2000. Molecular characterisation of the fibre-digesting bacteria isolated from the ostrich (Struthio Camelus var. Domesticus) hindgut. University of Cape Town.en_ZA
dc.identifier.ris TY - Thesis / Dissertation AU - Russouw, Tracy Karen Joe AB - Eleven bacterial strains that have been isolated from the ostrich hindgut, have successfully been identified with the aid of PCR amplification of the 16S rDNA genes and sequence analysis using the BLASTN search of the GenBank database. The nucleotide sequences were determined either through direct sequencing of the PCR products or after cloning the products into the plasmid vector pBluescript (SK). The results obtained from the 16S rDNA sequence homologies strongly suggests that the newly identified bacteria should be assigned to three major bacterial genera, namely, Ruminococcus, Buryrivibrio and Bacteroides. Four highly cellulolytic strains were identified as ruminococci while two weakly cellulolytic strains, showed highest homology to members of the genus Butyrivibrio. Another weakly cellulolytic strain showed closest identity to the sulfur reducing bacterial genus Desulfovibrio. Four non-cellulolytic strains showed highest homology to the genus Bacteroides. DA - 2000 DB - OpenUCT DP - University of Cape Town LK - https://open.uct.ac.za PB - University of Cape Town PY - 2000 T1 - Molecular characterisation of the fibre-digesting bacteria isolated from the ostrich (Struthio Camelus var. Domesticus) hindgut TI - Molecular characterisation of the fibre-digesting bacteria isolated from the ostrich (Struthio Camelus var. Domesticus) hindgut UR - http://hdl.handle.net/11427/9740 ER - en_ZA
dc.identifier.urihttp://hdl.handle.net/11427/9740
dc.identifier.vancouvercitationRussouw TKJ. Molecular characterisation of the fibre-digesting bacteria isolated from the ostrich (Struthio Camelus var. Domesticus) hindgut. [Thesis]. University of Cape Town ,Faculty of Science ,Department of Molecular and Cell Biology, 2000 [cited yyyy month dd]. Available from: http://hdl.handle.net/11427/9740en_ZA
dc.language.isoengen_ZA
dc.publisher.departmentDepartment of Molecular and Cell Biologyen_ZA
dc.publisher.facultyFaculty of Scienceen_ZA
dc.publisher.institutionUniversity of Cape Town
dc.subject.otherMicrobiologyen_ZA
dc.titleMolecular characterisation of the fibre-digesting bacteria isolated from the ostrich (Struthio Camelus var. Domesticus) hindguten_ZA
dc.typeMaster Thesis
dc.type.qualificationlevelMasters
dc.type.qualificationnameMPhilen_ZA
uct.type.filetypeText
uct.type.filetypeImage
uct.type.publicationResearchen_ZA
uct.type.resourceThesisen_ZA
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