Histone octamer helical tubes suggest that an internucleosomal four-helix bundle stabilizes the chromatin fiber.

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dc.contributor.authorFrouws, Timothy D
dc.contributor.authorPatterton, Hugh-G
dc.contributor.authorSewell, Bryan T
dc.date.accessioned2016-07-21T15:53:58Z
dc.date.available2016-07-21T15:53:58Z
dc.date.issued2009
dc.date.updated2016-07-21T15:52:49Z
dc.description.abstractA major question in chromatin involves the exact organization of nucleosomes within the 30-nm chromatin fiber and its structural determinants of assembly. Here we investigate the structure of histone octamer helical tubes via the method of iterative helical real-space reconstruction. Accurate placement of the x-ray structure of the histone octamer within the reconstructed density yields a pseudoatomic model for the entire helix, and allows precise identification of molecular interactions between neighboring octamers. One such interaction that would not be obscured by DNA in the nucleosome consists of a twofold symmetric four-helix bundle formed between pairs of H2B-a3 and H2B-aC helices of neighboring octamers. We believe that this interface can act as an internucleosomal four-helix bundle within the context of the chromatin fiber. The potential relevance of this interface in the folding of the 30-nm chromatin fiber is discussed.en_ZA
dc.identifierhttp://dx.doi.org/10.1016/j.bpj.2008.10.075
dc.identifier.apacitationFrouws, T. D., Patterton, H., & Sewell, B. T. (2009). Histone octamer helical tubes suggest that an internucleosomal four-helix bundle stabilizes the chromatin fiber. <i>Biophysical Journal</i>, http://hdl.handle.net/11427/20591en_ZA
dc.identifier.chicagocitationFrouws, Timothy D, Hugh-G Patterton, and Bryan T Sewell "Histone octamer helical tubes suggest that an internucleosomal four-helix bundle stabilizes the chromatin fiber." <i>Biophysical Journal</i> (2009) http://hdl.handle.net/11427/20591en_ZA
dc.identifier.citationFrouws, T. D., Patterton, H. G., & Sewell, B. T. (2009). Histone octamer helical tubes suggest that an internucleosomal four-helix bundle stabilizes the chromatin fiber. Biophysical journal, 96(8), 3363-3371.en_ZA
dc.identifier.issn0006-3495en_ZA
dc.identifier.ris TY - Journal Article AU - Frouws, Timothy D AU - Patterton, Hugh-G AU - Sewell, Bryan T AB - A major question in chromatin involves the exact organization of nucleosomes within the 30-nm chromatin fiber and its structural determinants of assembly. Here we investigate the structure of histone octamer helical tubes via the method of iterative helical real-space reconstruction. Accurate placement of the x-ray structure of the histone octamer within the reconstructed density yields a pseudoatomic model for the entire helix, and allows precise identification of molecular interactions between neighboring octamers. One such interaction that would not be obscured by DNA in the nucleosome consists of a twofold symmetric four-helix bundle formed between pairs of H2B-a3 and H2B-aC helices of neighboring octamers. We believe that this interface can act as an internucleosomal four-helix bundle within the context of the chromatin fiber. The potential relevance of this interface in the folding of the 30-nm chromatin fiber is discussed. DA - 2009 DB - OpenUCT DP - University of Cape Town J1 - Biophysical Journal LK - https://open.uct.ac.za PB - University of Cape Town PY - 2009 SM - 0006-3495 T1 - Histone octamer helical tubes suggest that an internucleosomal four-helix bundle stabilizes the chromatin fiber TI - Histone octamer helical tubes suggest that an internucleosomal four-helix bundle stabilizes the chromatin fiber UR - http://hdl.handle.net/11427/20591 ER - en_ZA
dc.identifier.urihttp://hdl.handle.net/11427/20591
dc.identifier.urihttp://www.cell.com/biophysj/abstract/S0006-3495(09)00487-1
dc.identifier.vancouvercitationFrouws TD, Patterton H, Sewell BT. Histone octamer helical tubes suggest that an internucleosomal four-helix bundle stabilizes the chromatin fiber. Biophysical Journal. 2009; http://hdl.handle.net/11427/20591.en_ZA
dc.languageengen_ZA
dc.publisherBiophysical Societyen_ZA
dc.publisher.institutionUniversity of Cape Town
dc.sourceBiophysical Journalen_ZA
dc.source.urihttp://www.cell.com/biophysj/home
dc.subject.otherinternucleosomal four-helix bundle
dc.subject.otherchromatin fiber.
dc.titleHistone octamer helical tubes suggest that an internucleosomal four-helix bundle stabilizes the chromatin fiber.en_ZA
dc.typeJournal Articleen_ZA
uct.type.filetypeText
uct.type.filetypeImage
uct.type.publicationResearchen_ZA
uct.type.resourceArticleen_ZA
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