Studies on Clostridium acetobutylicum P262 : sporulation induction and analysis of an oligosporogenous, solvent-deficient mutant

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dc.contributor.advisorReid, Shezen_ZA
dc.contributor.advisorWoods, Daveen_ZA
dc.contributor.authorBabb, Brendan Lloyden_ZA
dc.date.accessioned2016-08-18T13:53:23Z
dc.date.available2016-08-18T13:53:23Z
dc.date.issued1994en_ZA
dc.description.abstractThe ability of various fermentation products to induce sporulation was in order to design a sporulation induction medium for Clostridium acetobutylicum P262. Of acetic acid, butyric acetone and butanol, was found to be most effective at induction. Induction was more efficient at low pH values under certain conditions. The heat resistance of mature spores was determined and the optlmal temperature for spore quantification was shown to be 75·C. acetobutylicum mutants m5 06 were by transposon mutagenesis using the conjugative transposon Tn925::Tn917, not transposon Tn925 as previously thought [Babb. B.L. 1990. B.Sc. (Honours) thesis, University of Cape Town. South Africa]. The spore development and the fermentation profiles of mutants were in batch over a period of 60h. Mutant m5 was shown to be oligosporogenous with majority of cells blocked at sporulation stage H. It was deficient in acetone and butanol production. Mutant 06 proceeded to sporulation stage VII and produced acetone and butanol at levels similar to that of the wild type strain. Mutants 06 appeared to contain two respectively from Southern hybridization experiments using a probe for left transposon junction. However, when a probe to right transposon junction was used, the chromosomal deoxyribonucleic acid (DNA) of mutant m5 was shown to contain approximately eight junction sites. The cause for the anomalous hybridization pattern was non-specific restriction enzyme activity nor a result of independent transposition of transposon Tn917.en_ZA
dc.identifier.apacitationBabb, B. L. (1994). <i>Studies on Clostridium acetobutylicum P262 : sporulation induction and analysis of an oligosporogenous, solvent-deficient mutant</i>. (Thesis). University of Cape Town ,Faculty of Science ,Department of Molecular and Cell Biology. Retrieved from http://hdl.handle.net/11427/21336en_ZA
dc.identifier.chicagocitationBabb, Brendan Lloyd. <i>"Studies on Clostridium acetobutylicum P262 : sporulation induction and analysis of an oligosporogenous, solvent-deficient mutant."</i> Thesis., University of Cape Town ,Faculty of Science ,Department of Molecular and Cell Biology, 1994. http://hdl.handle.net/11427/21336en_ZA
dc.identifier.citationBabb, B. 1994. Studies on Clostridium acetobutylicum P262 : sporulation induction and analysis of an oligosporogenous, solvent-deficient mutant. University of Cape Town.en_ZA
dc.identifier.ris TY - Thesis / Dissertation AU - Babb, Brendan Lloyd AB - The ability of various fermentation products to induce sporulation was in order to design a sporulation induction medium for Clostridium acetobutylicum P262. Of acetic acid, butyric acetone and butanol, was found to be most effective at induction. Induction was more efficient at low pH values under certain conditions. The heat resistance of mature spores was determined and the optlmal temperature for spore quantification was shown to be 75·C. acetobutylicum mutants m5 06 were by transposon mutagenesis using the conjugative transposon Tn925::Tn917, not transposon Tn925 as previously thought [Babb. B.L. 1990. B.Sc. (Honours) thesis, University of Cape Town. South Africa]. The spore development and the fermentation profiles of mutants were in batch over a period of 60h. Mutant m5 was shown to be oligosporogenous with majority of cells blocked at sporulation stage H. It was deficient in acetone and butanol production. Mutant 06 proceeded to sporulation stage VII and produced acetone and butanol at levels similar to that of the wild type strain. Mutants 06 appeared to contain two respectively from Southern hybridization experiments using a probe for left transposon junction. However, when a probe to right transposon junction was used, the chromosomal deoxyribonucleic acid (DNA) of mutant m5 was shown to contain approximately eight junction sites. The cause for the anomalous hybridization pattern was non-specific restriction enzyme activity nor a result of independent transposition of transposon Tn917. DA - 1994 DB - OpenUCT DP - University of Cape Town LK - https://open.uct.ac.za PB - University of Cape Town PY - 1994 T1 - Studies on Clostridium acetobutylicum P262 : sporulation induction and analysis of an oligosporogenous, solvent-deficient mutant TI - Studies on Clostridium acetobutylicum P262 : sporulation induction and analysis of an oligosporogenous, solvent-deficient mutant UR - http://hdl.handle.net/11427/21336 ER - en_ZA
dc.identifier.urihttp://hdl.handle.net/11427/21336
dc.identifier.vancouvercitationBabb BL. Studies on Clostridium acetobutylicum P262 : sporulation induction and analysis of an oligosporogenous, solvent-deficient mutant. [Thesis]. University of Cape Town ,Faculty of Science ,Department of Molecular and Cell Biology, 1994 [cited yyyy month dd]. Available from: http://hdl.handle.net/11427/21336en_ZA
dc.language.isoengen_ZA
dc.publisher.departmentDepartment of Molecular and Cell Biologyen_ZA
dc.publisher.facultyFaculty of Scienceen_ZA
dc.publisher.institutionUniversity of Cape Town
dc.subject.otherMolecular and Cell Biologyen_ZA
dc.titleStudies on Clostridium acetobutylicum P262 : sporulation induction and analysis of an oligosporogenous, solvent-deficient mutanten_ZA
dc.typeMaster Thesis
dc.type.qualificationlevelMasters
dc.type.qualificationnameMScen_ZA
uct.type.filetypeText
uct.type.filetypeImage
uct.type.publicationResearchen_ZA
uct.type.resourceThesisen_ZA
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