Prevalence and antibiotic susceptibility patterns of enteric bacterial pathogens in human and non-human sources in an urban informal settlement in Cape Town, South Africa

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dc.contributor.authorKalule, John B
dc.contributor.authorSmith, Anthony M
dc.contributor.authorVulindhlu, Mjikisile
dc.contributor.authorTau, Nomsa P
dc.contributor.authorNicol, Mark P
dc.contributor.authorKeddy, Karen H
dc.contributor.authorRobberts, Lourens
dc.date.accessioned2019-12-10T08:55:15Z
dc.date.available2019-12-10T08:55:15Z
dc.date.issued2019-11-06
dc.date.updated2019-11-10T04:16:51Z
dc.description.abstractAbstract Background In light of rampant childhood diarrhoea, this study investigated bacterial pathogens from human and non-human sources in an urban informal settlement. Meat from informal abattoirs (n = 85), river water (n = 64), and diarrheic stool (n = 66) were collected between September 2015 and May 2016. A duplex real-time PCR, gel-based PCR, and CHROMagar™STEC were used to screen Tryptic Soy Broth (TSB) for diarrheic E. coli. Standard methods were used to screen for other selected food and waterborne bacterial pathogens. Results Pathogens isolated from stool, meat, and surface water included Salmonella enterica (6, 5, 0%), Plesiomonas shigelloides (9, 0, 17%), Aeromonas sobria (3, 3, 0%), Campylobacter jejuni (5, 5, 0%), Shigella flexneri (17, 5, 0%), Vibrio vulnificus (0, 0, 9%), and diarrheic E. coli (21, 3, 7%) respectively. All the isolates were resistant to trimethoprim–sulphamethoxazole. Conclusions There was a high burden of drug resistant diarrheal pathogens in the stool, surface water and meat from informal slaughter. Integrated control measures are needed to ensure food safety and to prevent the spread of drug resistant pathogens in similar settings.
dc.identifier.apacitationKalule, J. B., Smith, A. M., Vulindhlu, M., Tau, N. P., Nicol, M. P., Keddy, K. H., & Robberts, L. (2019). Prevalence and antibiotic susceptibility patterns of enteric bacterial pathogens in human and non-human sources in an urban informal settlement in Cape Town, South Africa. http://hdl.handle.net/11427/30671en_ZA
dc.identifier.chicagocitationKalule, John B, Anthony M Smith, Mjikisile Vulindhlu, Nomsa P Tau, Mark P Nicol, Karen H Keddy, and Lourens Robberts "Prevalence and antibiotic susceptibility patterns of enteric bacterial pathogens in human and non-human sources in an urban informal settlement in Cape Town, South Africa." (2019) http://hdl.handle.net/11427/30671en_ZA
dc.identifier.citationBMC Microbiology. 2019 Nov 06;19(1):244
dc.identifier.ris TY - Journal Article AU - Kalule, John B AU - Smith, Anthony M AU - Vulindhlu, Mjikisile AU - Tau, Nomsa P AU - Nicol, Mark P AU - Keddy, Karen H AU - Robberts, Lourens AB - Abstract Background In light of rampant childhood diarrhoea, this study investigated bacterial pathogens from human and non-human sources in an urban informal settlement. Meat from informal abattoirs (n = 85), river water (n = 64), and diarrheic stool (n = 66) were collected between September 2015 and May 2016. A duplex real-time PCR, gel-based PCR, and CHROMagar™STEC were used to screen Tryptic Soy Broth (TSB) for diarrheic E. coli. Standard methods were used to screen for other selected food and waterborne bacterial pathogens. Results Pathogens isolated from stool, meat, and surface water included Salmonella enterica (6, 5, 0%), Plesiomonas shigelloides (9, 0, 17%), Aeromonas sobria (3, 3, 0%), Campylobacter jejuni (5, 5, 0%), Shigella flexneri (17, 5, 0%), Vibrio vulnificus (0, 0, 9%), and diarrheic E. coli (21, 3, 7%) respectively. All the isolates were resistant to trimethoprim–sulphamethoxazole. Conclusions There was a high burden of drug resistant diarrheal pathogens in the stool, surface water and meat from informal slaughter. Integrated control measures are needed to ensure food safety and to prevent the spread of drug resistant pathogens in similar settings. DA - 2019-11-06 DB - OpenUCT DP - University of Cape Town KW - Informal settlement KW - Foodborne pathogens KW - Antibiograms KW - Diarrhoea LK - https://open.uct.ac.za PY - 2019 T1 - Prevalence and antibiotic susceptibility patterns of enteric bacterial pathogens in human and non-human sources in an urban informal settlement in Cape Town, South Africa TI - Prevalence and antibiotic susceptibility patterns of enteric bacterial pathogens in human and non-human sources in an urban informal settlement in Cape Town, South Africa UR - http://hdl.handle.net/11427/30671 ER - en_ZA
dc.identifier.urihttps://doi.org/10.1186/s12866-019-1620-6
dc.identifier.urihttp://hdl.handle.net/11427/30671
dc.identifier.vancouvercitationKalule JB, Smith AM, Vulindhlu M, Tau NP, Nicol MP, Keddy KH, et al. Prevalence and antibiotic susceptibility patterns of enteric bacterial pathogens in human and non-human sources in an urban informal settlement in Cape Town, South Africa. 2019; http://hdl.handle.net/11427/30671.en_ZA
dc.language.rfc3066en
dc.rights.holderThe Author(s).
dc.subjectInformal settlement
dc.subjectFoodborne pathogens
dc.subjectAntibiograms
dc.subjectDiarrhoea
dc.titlePrevalence and antibiotic susceptibility patterns of enteric bacterial pathogens in human and non-human sources in an urban informal settlement in Cape Town, South Africa
dc.typeJournal Article
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