The cloning of novel gonadotropin-releasing hormone receptors by polymerase chain reaction

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dc.contributor.authorHutchinson, Emerentiaen_ZA
dc.date.accessioned2018-01-25T13:53:58Z
dc.date.available2018-01-25T13:53:58Z
dc.date.issued1998en_ZA
dc.description.abstractGonadotropin-releasing hormone (GnRH), a central regulator of reproductive function in all vertebrates, exerts its effects via binding to the GnRH receptor (GnRHR) in the pituitary gonadotrophs. The GnRHR is a member of the G-protein coupled receptor (GPCR) superfarnily. A second form of the GnRHR (type II), other than the pituitary gonadotrope GnRHR (type I) has been proposed to exist and to play a role other than the classical endocrine role of the pituitary GnRHR. Elucidation of amino acid residues of the GnRHR that are crucial for ligand binding, activation of the receptor, and coupling to the G-protein, is important in understanding structure-function relationships towards the design of drugs for therapeutic intervention. Such information can often be deduced by a comparison between conserved and non-conserved amino acid residues of GnRHRs from different species. At the start of this project no non-mammalian or invertebrate, and only some of the eutherian mammalian type I GnRHRs had been cloned. The aim of this project was to clone novel GnRHRs, i.e. type I and type II GnRHRs from redbait and mole and type II mouse and human GnRHRs using polymerase chain reaction (PCR) strategies. PCR was performed with degenerate primers designed to human type I GnRHR to areas that are not conserved between GPCRs in general, but are conserved between mammalian GnRHRs.en_ZA
dc.identifier.apacitationHutchinson, E. (1998). <i>The cloning of novel gonadotropin-releasing hormone receptors by polymerase chain reaction</i>. (Thesis). University of Cape Town ,Faculty of Health Sciences ,Division of Chemical Pathology. Retrieved from http://hdl.handle.net/11427/26968en_ZA
dc.identifier.chicagocitationHutchinson, Emerentia. <i>"The cloning of novel gonadotropin-releasing hormone receptors by polymerase chain reaction."</i> Thesis., University of Cape Town ,Faculty of Health Sciences ,Division of Chemical Pathology, 1998. http://hdl.handle.net/11427/26968en_ZA
dc.identifier.citationHutchinson, E. 1998. The cloning of novel gonadotropin-releasing hormone receptors by polymerase chain reaction. University of Cape Town.en_ZA
dc.identifier.ris TY - Thesis / Dissertation AU - Hutchinson, Emerentia AB - Gonadotropin-releasing hormone (GnRH), a central regulator of reproductive function in all vertebrates, exerts its effects via binding to the GnRH receptor (GnRHR) in the pituitary gonadotrophs. The GnRHR is a member of the G-protein coupled receptor (GPCR) superfarnily. A second form of the GnRHR (type II), other than the pituitary gonadotrope GnRHR (type I) has been proposed to exist and to play a role other than the classical endocrine role of the pituitary GnRHR. Elucidation of amino acid residues of the GnRHR that are crucial for ligand binding, activation of the receptor, and coupling to the G-protein, is important in understanding structure-function relationships towards the design of drugs for therapeutic intervention. Such information can often be deduced by a comparison between conserved and non-conserved amino acid residues of GnRHRs from different species. At the start of this project no non-mammalian or invertebrate, and only some of the eutherian mammalian type I GnRHRs had been cloned. The aim of this project was to clone novel GnRHRs, i.e. type I and type II GnRHRs from redbait and mole and type II mouse and human GnRHRs using polymerase chain reaction (PCR) strategies. PCR was performed with degenerate primers designed to human type I GnRHR to areas that are not conserved between GPCRs in general, but are conserved between mammalian GnRHRs. DA - 1998 DB - OpenUCT DP - University of Cape Town LK - https://open.uct.ac.za PB - University of Cape Town PY - 1998 T1 - The cloning of novel gonadotropin-releasing hormone receptors by polymerase chain reaction TI - The cloning of novel gonadotropin-releasing hormone receptors by polymerase chain reaction UR - http://hdl.handle.net/11427/26968 ER - en_ZA
dc.identifier.urihttp://hdl.handle.net/11427/26968
dc.identifier.vancouvercitationHutchinson E. The cloning of novel gonadotropin-releasing hormone receptors by polymerase chain reaction. [Thesis]. University of Cape Town ,Faculty of Health Sciences ,Division of Chemical Pathology, 1998 [cited yyyy month dd]. Available from: http://hdl.handle.net/11427/26968en_ZA
dc.language.isoengen_ZA
dc.publisher.departmentDivision of Chemical Pathologyen_ZA
dc.publisher.facultyFaculty of Health Sciencesen_ZA
dc.publisher.institutionUniversity of Cape Town
dc.subject.otherChemical Pathologyen_ZA
dc.titleThe cloning of novel gonadotropin-releasing hormone receptors by polymerase chain reactionen_ZA
dc.typeMaster Thesis
dc.type.qualificationlevelMasters
dc.type.qualificationnameMSc (Med)en_ZA
uct.type.filetypeText
uct.type.filetypeImage
uct.type.publicationResearchen_ZA
uct.type.resourceThesisen_ZA
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