Microbial solvent formation revisited by comparative genome analysis

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dc.contributor.authorDYrre, Peter
dc.date.accessioned2021-10-08T06:20:17Z
dc.date.available2021-10-08T06:20:17Z
dc.date.issued2017
dc.description.abstractAbstract Background Microbial formation of acetone, isopropanol, and butanol is largely restricted to bacteria belonging to the genus Clostridium. This ability has been industrially exploited over the last 100 years. The solvents are important feedstocks for the chemical and biofuel industry. However, biological synthesis suffers from high substrate costs and competition from chemical synthesis supported by the low price of crude oil. To render the biotechnological production economically viable again, improvements in microbial and fermentation performance are necessary. However, no comprehensive comparisons of respective species and strains used and their specific abilities exist today. Results The genomes of a total 30 saccharolytic Clostridium strains, representative of the species Clostridium acetobutylicum, C. aurantibutyricum, C. beijerinckii, C. diolis, C. felsineum, C. pasteurianum, C. puniceum, C. roseum, C. saccharobutylicum, and C. saccharoperbutylacetonicum, have been determined; 10 of them completely, and compared to 14 published genomes of other solvent-forming clostridia. Two major groups could be differentiated and several misclassified species were detected. Conclusions Our findings represent a comprehensive study of phylogeny and taxonomy of clostridial solvent producers that highlights differences in energy conservation mechanisms and substrate utilization between strains, and allow for the first time a direct comparison of sequentially selected industrial strains at the genetic level. Detailed data mining is now possible, supporting the identification of new engineering targets for improved solvent production.
dc.identifier.apacitationDYrre, P. (2017). Microbial solvent formation revisited by comparative genome analysis. <i>Biotechnology for Biofuels</i>, 10(1), 174 - 177. http://hdl.handle.net/11427/34244en_ZA
dc.identifier.chicagocitationDYrre, Peter "Microbial solvent formation revisited by comparative genome analysis." <i>Biotechnology for Biofuels</i> 10, 1. (2017): 174 - 177. http://hdl.handle.net/11427/34244en_ZA
dc.identifier.citationDYrre, P. 2017. Microbial solvent formation revisited by comparative genome analysis. <i>Biotechnology for Biofuels.</i> 10(1):174 - 177. http://hdl.handle.net/11427/34244en_ZA
dc.identifier.issn1754-6834
dc.identifier.ris TY - Journal Article AU - DYrre, Peter AB - Abstract Background Microbial formation of acetone, isopropanol, and butanol is largely restricted to bacteria belonging to the genus Clostridium. This ability has been industrially exploited over the last 100 years. The solvents are important feedstocks for the chemical and biofuel industry. However, biological synthesis suffers from high substrate costs and competition from chemical synthesis supported by the low price of crude oil. To render the biotechnological production economically viable again, improvements in microbial and fermentation performance are necessary. However, no comprehensive comparisons of respective species and strains used and their specific abilities exist today. Results The genomes of a total 30 saccharolytic Clostridium strains, representative of the species Clostridium acetobutylicum, C. aurantibutyricum, C. beijerinckii, C. diolis, C. felsineum, C. pasteurianum, C. puniceum, C. roseum, C. saccharobutylicum, and C. saccharoperbutylacetonicum, have been determined; 10 of them completely, and compared to 14 published genomes of other solvent-forming clostridia. Two major groups could be differentiated and several misclassified species were detected. Conclusions Our findings represent a comprehensive study of phylogeny and taxonomy of clostridial solvent producers that highlights differences in energy conservation mechanisms and substrate utilization between strains, and allow for the first time a direct comparison of sequentially selected industrial strains at the genetic level. Detailed data mining is now possible, supporting the identification of new engineering targets for improved solvent production. DA - 2017 DB - OpenUCT DP - University of Cape Town IS - 1 J1 - Biotechnology for Biofuels LK - https://open.uct.ac.za PY - 2017 SM - 1754-6834 T1 - Microbial solvent formation revisited by comparative genome analysis TI - Microbial solvent formation revisited by comparative genome analysis UR - http://hdl.handle.net/11427/34244 ER - en_ZA
dc.identifier.urihttp://hdl.handle.net/11427/34244
dc.identifier.vancouvercitationDYrre P. Microbial solvent formation revisited by comparative genome analysis. Biotechnology for Biofuels. 2017;10(1):174 - 177. http://hdl.handle.net/11427/34244.en_ZA
dc.language.isoeng
dc.publisher.departmentDepartment of Molecular and Cell Biology
dc.publisher.facultyFaculty of Science
dc.sourceBiotechnology for Biofuels
dc.source.journalissue1
dc.source.journalvolume10
dc.source.pagination174 - 177
dc.source.urihttps://dx.doi.org/10.1186/s13068-017-0742-z
dc.subject.otherBiotechnology
dc.subject.otherPlant Breeding/Biotechnology
dc.subject.otherEnvironmental Engineering/Biotechnology
dc.subject.otherRenewable and Green Energy
dc.subject.otherMicrobiology
dc.subject.othersynthesis
dc.subject.otherClostridium acetobutylicum
dc.subject.otheracetone
dc.subject.otherbacteria
dc.subject.otherbioenergy industry
dc.subject.otherfeedstocks
dc.subject.otherengineering
dc.subject.othertaxonomy
dc.subject.otherbutanol
dc.subject.othergenome
dc.subject.otherenergy conservation
dc.subject.otherphylogeny
dc.subject.otherfermentation
dc.subject.othersolvents
dc.subject.othersequence analysis
dc.subject.otherpetroleum
dc.subject.otherprices
dc.subject.otherisopropyl alcohol
dc.subject.othereconomic sustainability
dc.titleMicrobial solvent formation revisited by comparative genome analysis
dc.typeJournal Article
uct.type.publicationResearch
uct.type.resourceJournal Article
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