The development of aptamer-based probes for the detection of TB antigens ESAT-6.CFP-10 potential TB diagnostic tools

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dc.contributor.advisorDheda, Keertanen_ZA
dc.contributor.advisorKhati, Makobetsaen_ZA
dc.contributor.advisorGresh, Lionelen_ZA
dc.contributor.authorMaserumule, Matsopiane Charlotteen_ZA
dc.date.accessioned2014-07-29T09:07:14Z
dc.date.available2014-07-29T09:07:14Z
dc.date.issued2013en_ZA
dc.descriptionIncludes abstract.
dc.descriptionIncludes bibliographical references.
dc.description.abstractLack of point-of-care (PoC) diagnostic tools for TB hinders control of the disease, particularly in resource-limited, high HIV and TB prevalence countries. Therefore, there is a need for simple, rapid, accurate, and affordable PoC diagnostics to detect active TB early enough for opportune intervention. To develop TB detection probes that will constitute such diagnostics, our research group recently isolated DNA aptamers that bind to a putative marker for active TB; the ESAT-6.CFP-10 heterodimer. Aptamers are highly specific artificial mimics of antibodies that have shown great prospects in diagnostic applications. The aim of this study was to characterise the anti-ESAT-6.CFP-10 aptamers, and to optimise them into more specific and affordable detection probes for the development of potential PoC TB diagnostic tools.en_ZA
dc.identifier.apacitationMaserumule, M. C. (2013). <i>The development of aptamer-based probes for the detection of TB antigens ESAT-6.CFP-10 potential TB diagnostic tools</i>. (Thesis). University of Cape Town ,Faculty of Health Sciences ,Department of Medicine. Retrieved from http://hdl.handle.net/11427/3425en_ZA
dc.identifier.chicagocitationMaserumule, Matsopiane Charlotte. <i>"The development of aptamer-based probes for the detection of TB antigens ESAT-6.CFP-10 potential TB diagnostic tools."</i> Thesis., University of Cape Town ,Faculty of Health Sciences ,Department of Medicine, 2013. http://hdl.handle.net/11427/3425en_ZA
dc.identifier.citationMaserumule, M. 2013. The development of aptamer-based probes for the detection of TB antigens ESAT-6.CFP-10 potential TB diagnostic tools. University of Cape Town.en_ZA
dc.identifier.ris TY - Thesis / Dissertation AU - Maserumule, Matsopiane Charlotte AB - Lack of point-of-care (PoC) diagnostic tools for TB hinders control of the disease, particularly in resource-limited, high HIV and TB prevalence countries. Therefore, there is a need for simple, rapid, accurate, and affordable PoC diagnostics to detect active TB early enough for opportune intervention. To develop TB detection probes that will constitute such diagnostics, our research group recently isolated DNA aptamers that bind to a putative marker for active TB; the ESAT-6.CFP-10 heterodimer. Aptamers are highly specific artificial mimics of antibodies that have shown great prospects in diagnostic applications. The aim of this study was to characterise the anti-ESAT-6.CFP-10 aptamers, and to optimise them into more specific and affordable detection probes for the development of potential PoC TB diagnostic tools. DA - 2013 DB - OpenUCT DP - University of Cape Town LK - https://open.uct.ac.za PB - University of Cape Town PY - 2013 T1 - The development of aptamer-based probes for the detection of TB antigens ESAT-6.CFP-10 potential TB diagnostic tools TI - The development of aptamer-based probes for the detection of TB antigens ESAT-6.CFP-10 potential TB diagnostic tools UR - http://hdl.handle.net/11427/3425 ER - en_ZA
dc.identifier.urihttp://hdl.handle.net/11427/3425
dc.identifier.vancouvercitationMaserumule MC. The development of aptamer-based probes for the detection of TB antigens ESAT-6.CFP-10 potential TB diagnostic tools. [Thesis]. University of Cape Town ,Faculty of Health Sciences ,Department of Medicine, 2013 [cited yyyy month dd]. Available from: http://hdl.handle.net/11427/3425en_ZA
dc.language.isoengen_ZA
dc.publisher.departmentDepartment of Medicineen_ZA
dc.publisher.facultyFaculty of Health Sciencesen_ZA
dc.publisher.institutionUniversity of Cape Town
dc.subject.otherMedicineen_ZA
dc.titleThe development of aptamer-based probes for the detection of TB antigens ESAT-6.CFP-10 potential TB diagnostic toolsen_ZA
dc.typeMaster Thesis
dc.type.qualificationlevelMasters
dc.type.qualificationnameMScen_ZA
uct.type.filetypeText
uct.type.filetypeImage
uct.type.publicationResearchen_ZA
uct.type.resourceThesisen_ZA
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