A study of the molecular variability of some South African isolates of tobacco necrosis virus

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dc.contributor.advisorVon Wechmar, M Barbaraen_ZA
dc.contributor.advisorPurves, Men_ZA
dc.contributor.authorFreeborough, Michael-Johnen_ZA
dc.date.accessioned2016-07-04T08:42:26Z
dc.date.available2016-07-04T08:42:26Z
dc.date.issued1996en_ZA
dc.descriptionBibliography: pages 109-118.en_ZA
dc.description.abstractThe relationship of various tobacco necrosis viruses, isolated from various crops and other sources in South Africa was determined. An isolate from avocado was chosen for partial characterisation to confirm that it was a member of the Necrovirus group of plant viruses. TNV was detected in potatoes that exhibited abnormal necrotic foliar symptoms and a D-type TNV was isolated and identified from the Up-to-Date potato variety. Immunoelectroblotting assay grouped the TNV isolates studied into two serotypes (A and D). This result was confirmed by NA hybridization with probes derived to the coat protein of an A- (TNVWheat) and a D-type (TNV-Papaya green lesion) isolate. RT-PCR with A and D specific primers did not amplify the coat protein of three A- and D-type TNV isolates which appears to indicate that the detection by PCR with specific primers is too selective to be used for a general test, unless degenerate primers to a more conserved region of the coat protein gene are used. Sequence analysis of the coat protein gene was used to determine the phylogenetic relationship amongst nine TNV isolates examined and also by comparison to three isolates for which the coat protein gene had already been sequenced. Sequence analysis showed high degree of homology amongst the A-type isolates and the D-type isolates, with approximately 45 % homology between the two TNV types.en_ZA
dc.identifier.apacitationFreeborough, M. (1996). <i>A study of the molecular variability of some South African isolates of tobacco necrosis virus</i>. (Thesis). University of Cape Town ,Faculty of Science ,Department of Molecular and Cell Biology. Retrieved from http://hdl.handle.net/11427/20192en_ZA
dc.identifier.chicagocitationFreeborough, Michael-John. <i>"A study of the molecular variability of some South African isolates of tobacco necrosis virus."</i> Thesis., University of Cape Town ,Faculty of Science ,Department of Molecular and Cell Biology, 1996. http://hdl.handle.net/11427/20192en_ZA
dc.identifier.citationFreeborough, M. 1996. A study of the molecular variability of some South African isolates of tobacco necrosis virus. University of Cape Town.en_ZA
dc.identifier.ris TY - Thesis / Dissertation AU - Freeborough, Michael-John AB - The relationship of various tobacco necrosis viruses, isolated from various crops and other sources in South Africa was determined. An isolate from avocado was chosen for partial characterisation to confirm that it was a member of the Necrovirus group of plant viruses. TNV was detected in potatoes that exhibited abnormal necrotic foliar symptoms and a D-type TNV was isolated and identified from the Up-to-Date potato variety. Immunoelectroblotting assay grouped the TNV isolates studied into two serotypes (A and D). This result was confirmed by NA hybridization with probes derived to the coat protein of an A- (TNVWheat) and a D-type (TNV-Papaya green lesion) isolate. RT-PCR with A and D specific primers did not amplify the coat protein of three A- and D-type TNV isolates which appears to indicate that the detection by PCR with specific primers is too selective to be used for a general test, unless degenerate primers to a more conserved region of the coat protein gene are used. Sequence analysis of the coat protein gene was used to determine the phylogenetic relationship amongst nine TNV isolates examined and also by comparison to three isolates for which the coat protein gene had already been sequenced. Sequence analysis showed high degree of homology amongst the A-type isolates and the D-type isolates, with approximately 45 % homology between the two TNV types. DA - 1996 DB - OpenUCT DP - University of Cape Town LK - https://open.uct.ac.za PB - University of Cape Town PY - 1996 T1 - A study of the molecular variability of some South African isolates of tobacco necrosis virus TI - A study of the molecular variability of some South African isolates of tobacco necrosis virus UR - http://hdl.handle.net/11427/20192 ER - en_ZA
dc.identifier.urihttp://hdl.handle.net/11427/20192
dc.identifier.vancouvercitationFreeborough M. A study of the molecular variability of some South African isolates of tobacco necrosis virus. [Thesis]. University of Cape Town ,Faculty of Science ,Department of Molecular and Cell Biology, 1996 [cited yyyy month dd]. Available from: http://hdl.handle.net/11427/20192en_ZA
dc.language.isoengen_ZA
dc.publisher.departmentDepartment of Molecular and Cell Biologyen_ZA
dc.publisher.facultyFaculty of Scienceen_ZA
dc.publisher.institutionUniversity of Cape Town
dc.subject.otherMolecular and Cell Biologyen_ZA
dc.titleA study of the molecular variability of some South African isolates of tobacco necrosis virusen_ZA
dc.typeMaster Thesis
dc.type.qualificationlevelMasters
dc.type.qualificationnameMScen_ZA
uct.type.filetypeText
uct.type.filetypeImage
uct.type.publicationResearchen_ZA
uct.type.resourceThesisen_ZA
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