Influence of Tumour Necrosis Factor Alpha on the Outcome of Ischaemic Postconditioning in the Presence of Obesity and Diabetes

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dc.contributor.authorLacerda, Lydia
dc.contributor.authorOpie, Lionel H
dc.contributor.authorLecour, Sandrine
dc.date.accessioned2021-10-08T07:04:05Z
dc.date.available2021-10-08T07:04:05Z
dc.date.issued2012
dc.description.abstractObesity and diabetes contribute to cardiovascular disease and alter cytokine profile. The cytokine, tumour necrosis factor alpha (TNFα), activates a protective signalling cascade during ischaemic postconditioning (IPostC). However, most successful clinical studies with IPostC have not included obese and/or diabetic patients. We aimed to investigate the influence of TNFα on the outcome of IPostC in obese or diabetic mice. TNF knockout or wildtype mice were fed for 11 weeks with a high carbohydrate diet (HCD) to induce modest obesity. Diabetes was induced in a separate group by administration of a single intraperitoneal injection of streptozotocin. Hearts were then isolated and subjected to ischaemia (35 min of global ischaemia) followed by 45 min of reperfusion. HCD increased body weight, plasma insulin and leptin levels while the glucose level was unchanged. In streptozotocin-treated mice, blood glucose, plasma leptin and insulin were altered. Control, obese or diabetic mice were protected with IPostC in wiltype animals. In TNF knockout mice, IPostC failed to protect control and diabetic hearts while a slight protection was observed in obese hearts. Our data confirm a bidirectional role for TNFα associated with the severity of concomitant comorbidities and suggest that diabetic and/or modestly obese patients may still benefit from IPostC.
dc.identifier.apacitationLacerda, L., Opie, L. H., & Lecour, S. (2012). Influence of Tumour Necrosis Factor Alpha on the Outcome of Ischaemic Postconditioning in the Presence of Obesity and Diabetes. <i>Experimental Diabetes Research</i>, 2012(4), 174 - 177. http://hdl.handle.net/11427/34422en_ZA
dc.identifier.chicagocitationLacerda, Lydia, Lionel H Opie, and Sandrine Lecour "Influence of Tumour Necrosis Factor Alpha on the Outcome of Ischaemic Postconditioning in the Presence of Obesity and Diabetes." <i>Experimental Diabetes Research</i> 2012, 4. (2012): 174 - 177. http://hdl.handle.net/11427/34422en_ZA
dc.identifier.citationLacerda, L., Opie, L.H. & Lecour, S. 2012. Influence of Tumour Necrosis Factor Alpha on the Outcome of Ischaemic Postconditioning in the Presence of Obesity and Diabetes. <i>Experimental Diabetes Research.</i> 2012(4):174 - 177. http://hdl.handle.net/11427/34422en_ZA
dc.identifier.issn1687-5214
dc.identifier.issn1687-5303
dc.identifier.ris TY - Journal Article AU - Lacerda, Lydia AU - Opie, Lionel H AU - Lecour, Sandrine AB - Obesity and diabetes contribute to cardiovascular disease and alter cytokine profile. The cytokine, tumour necrosis factor alpha (TNFα), activates a protective signalling cascade during ischaemic postconditioning (IPostC). However, most successful clinical studies with IPostC have not included obese and/or diabetic patients. We aimed to investigate the influence of TNFα on the outcome of IPostC in obese or diabetic mice. TNF knockout or wildtype mice were fed for 11 weeks with a high carbohydrate diet (HCD) to induce modest obesity. Diabetes was induced in a separate group by administration of a single intraperitoneal injection of streptozotocin. Hearts were then isolated and subjected to ischaemia (35 min of global ischaemia) followed by 45 min of reperfusion. HCD increased body weight, plasma insulin and leptin levels while the glucose level was unchanged. In streptozotocin-treated mice, blood glucose, plasma leptin and insulin were altered. Control, obese or diabetic mice were protected with IPostC in wiltype animals. In TNF knockout mice, IPostC failed to protect control and diabetic hearts while a slight protection was observed in obese hearts. Our data confirm a bidirectional role for TNFα associated with the severity of concomitant comorbidities and suggest that diabetic and/or modestly obese patients may still benefit from IPostC. DA - 2012 DB - OpenUCT DP - University of Cape Town IS - 4 J1 - Experimental Diabetes Research LK - https://open.uct.ac.za PY - 2012 SM - 1687-5214 SM - 1687-5303 T1 - Influence of Tumour Necrosis Factor Alpha on the Outcome of Ischaemic Postconditioning in the Presence of Obesity and Diabetes TI - Influence of Tumour Necrosis Factor Alpha on the Outcome of Ischaemic Postconditioning in the Presence of Obesity and Diabetes UR - http://hdl.handle.net/11427/34422 ER - en_ZA
dc.identifier.urihttp://hdl.handle.net/11427/34422
dc.identifier.vancouvercitationLacerda L, Opie LH, Lecour S. Influence of Tumour Necrosis Factor Alpha on the Outcome of Ischaemic Postconditioning in the Presence of Obesity and Diabetes. Experimental Diabetes Research. 2012;2012(4):174 - 177. http://hdl.handle.net/11427/34422.en_ZA
dc.language.isoeng
dc.publisher.departmentHatter Institute for Cardiovascular Research in Africa
dc.publisher.facultyFaculty of Health Sciences
dc.sourceExperimental Diabetes Research
dc.source.journalissue4
dc.source.journalvolume2012
dc.source.pagination174 - 177
dc.source.urihttps://dx.doi.org/10.1155/2012/502654
dc.subject.otherAnimals
dc.subject.otherCardiomegaly
dc.subject.otherDiabetes Mellitus, Type 1
dc.subject.otherDietary Carbohydrates
dc.subject.otherDisease Susceptibility
dc.subject.otherHeart
dc.subject.otherHyperglycemia
dc.subject.otherHyperinsulinism
dc.subject.otherIn Vitro Techniques
dc.subject.otherIschemic Postconditioning
dc.subject.otherLeptin
dc.subject.otherMice
dc.subject.otherMice, Knockout
dc.subject.otherMyocardial Ischemia
dc.titleInfluence of Tumour Necrosis Factor Alpha on the Outcome of Ischaemic Postconditioning in the Presence of Obesity and Diabetes
dc.typeJournal Article
uct.type.publicationResearch
uct.type.resourceJournal Article
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