An investigation into the role of cytosolic free Ca2+ in Salicylic acid mediation of disease resistance in Arabidopsis

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dc.contributor.advisorDenby, Katherine J
dc.contributor.authorPetersen, Lindsay Natalie
dc.date.accessioned2024-07-04T13:43:30Z
dc.date.available2024-07-04T13:43:30Z
dc.date.issued2001
dc.date.updated2024-07-02T13:14:55Z
dc.description.abstractSalicylic acid (SA) accumulation upon pathogen attack is a fundamental requirement for the activation of numerous plant defence mechansms. Cytosolic free Ca2+ ([Ca2+]c) is a ubiquitous signalling molecule involved in a host of cellular processes. Using transgenic Arabidopsis thaliana seedlings expressing the Ca2+-sensitive photoprotein aequorin, we previously reported a rapid and transient increase in [Ca2+]c upon application of exogenous SA. We now investigated the characteristics of the SA-induced [Ca2+]c increase and report that the majority of the response is derived from internal stores. It appears likely that SA triggers Ca2+-induced Ca2+-release. Preliminary evidence suggests a role for the SA-induced [Ca2+]c increase in the regulation of NPR1 expression since modulation of the SA-induced [Ca2+]c response with the extracellular Ca2+ chelator BAPTA causes a reduction in NPR1 mRNA levels. We have isolated two putative mutants that are defective in their ability to produce a SA-induced [Ca2+]c increase. Characterisation of these mutants is underway and will prove invaluable in identifying the components or events that cause the SA-induced [Ca2+]c transient, thereby aiding in the understanding of the role of [Ca2+]c in SA-mediated signal transduction.
dc.identifier.apacitationPetersen, L. N. (2001). <i>An investigation into the role of cytosolic free Ca2+ in Salicylic acid mediation of disease resistance in Arabidopsis</i>. (). ,Faculty of Science ,Department of Molecular and Cell Biology. Retrieved from http://hdl.handle.net/11427/40285en_ZA
dc.identifier.chicagocitationPetersen, Lindsay Natalie. <i>"An investigation into the role of cytosolic free Ca2+ in Salicylic acid mediation of disease resistance in Arabidopsis."</i> ., ,Faculty of Science ,Department of Molecular and Cell Biology, 2001. http://hdl.handle.net/11427/40285en_ZA
dc.identifier.citationPetersen, L.N. 2001. An investigation into the role of cytosolic free Ca2+ in Salicylic acid mediation of disease resistance in Arabidopsis. . ,Faculty of Science ,Department of Molecular and Cell Biology. http://hdl.handle.net/11427/40285en_ZA
dc.identifier.ris TY - Thesis / Dissertation AU - Petersen, Lindsay Natalie AB - Salicylic acid (SA) accumulation upon pathogen attack is a fundamental requirement for the activation of numerous plant defence mechansms. Cytosolic free Ca2+ ([Ca2+]c) is a ubiquitous signalling molecule involved in a host of cellular processes. Using transgenic Arabidopsis thaliana seedlings expressing the Ca2+-sensitive photoprotein aequorin, we previously reported a rapid and transient increase in [Ca2+]c upon application of exogenous SA. We now investigated the characteristics of the SA-induced [Ca2+]c increase and report that the majority of the response is derived from internal stores. It appears likely that SA triggers Ca2+-induced Ca2+-release. Preliminary evidence suggests a role for the SA-induced [Ca2+]c increase in the regulation of NPR1 expression since modulation of the SA-induced [Ca2+]c response with the extracellular Ca2+ chelator BAPTA causes a reduction in NPR1 mRNA levels. We have isolated two putative mutants that are defective in their ability to produce a SA-induced [Ca2+]c increase. Characterisation of these mutants is underway and will prove invaluable in identifying the components or events that cause the SA-induced [Ca2+]c transient, thereby aiding in the understanding of the role of [Ca2+]c in SA-mediated signal transduction. DA - 2001 DB - OpenUCT DP - University of Cape Town KW - Molecular and Cell Biology LK - https://open.uct.ac.za PY - 2001 T1 - An investigation into the role of cytosolic free Ca2+ in Salicylic acid mediation of disease resistance in Arabidopsis TI - An investigation into the role of cytosolic free Ca2+ in Salicylic acid mediation of disease resistance in Arabidopsis UR - http://hdl.handle.net/11427/40285 ER - en_ZA
dc.identifier.urihttp://hdl.handle.net/11427/40285
dc.identifier.vancouvercitationPetersen LN. An investigation into the role of cytosolic free Ca2+ in Salicylic acid mediation of disease resistance in Arabidopsis. []. ,Faculty of Science ,Department of Molecular and Cell Biology, 2001 [cited yyyy month dd]. Available from: http://hdl.handle.net/11427/40285en_ZA
dc.language.rfc3066eng
dc.publisher.departmentDepartment of Molecular and Cell Biology
dc.publisher.facultyFaculty of Science
dc.subjectMolecular and Cell Biology
dc.titleAn investigation into the role of cytosolic free Ca2+ in Salicylic acid mediation of disease resistance in Arabidopsis
dc.typeThesis / Dissertation
dc.type.qualificationlevelMasters
dc.type.qualificationlevelMSc
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