The development of a flow cytometric method to detect the presence of mutated nucleophosmin in Acute Myeloid Leukaemia

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dc.contributor.advisorShires, Karenen_ZA
dc.contributor.authorDu Pisani, Louis Almeroen_ZA
dc.date.accessioned2014-08-15T14:04:37Z
dc.date.available2014-08-15T14:04:37Z
dc.date.issued2014en_ZA
dc.descriptionIncludes abstract.en_ZA
dc.descriptionIncludes bibliographical references.en_ZA
dc.description.abstractNucleophosmin (NPM1) is an acidic, nucleo-cytoplasmic, shuttling protein with predominant nucleolar localisation that plays multiple roles in cell growth and proliferation. Deletion insertion mutations of NPM1 (NPM1 DIM) seem to disrupt it normal physiologic role as a molecular chaperone, which likely leads to its oncogenic potential.NPM1 if present alone (not associated with FLT3 internal tandem duplications (FLT3-ITD)) is associated with significantly better overall survival and disease free survival in AML and has been entered as a provisional category in the World Health Organisation (2008) classification of Acute Myeloid Leukaemia with recurrent genetic abnormalities. Current methodology uses reverse transcriptase polymerase chain reaction (RT-PCR) and genomic deoxyribonucleic acid (DNA) PCR techniques to detect NPM1 DIM. Although these methods are robust and relatively easy to perform they can be expensive, labour intensive and not universally available. Six major variants of NPM1 DIM (Types A-F) have been described all leading to frame shift. All six types share the same last five amino acids in the C-terminal.The aim of this study was to develop a robust flow cytometry methodology that could be used in the routine assessment of AML samples to determine the mutational state of NPM, using a commercially available polyclonal antibody against the mutated NPM1.en_ZA
dc.identifier.apacitationDu Pisani, L. A. (2014). <i>The development of a flow cytometric method to detect the presence of mutated nucleophosmin in Acute Myeloid Leukaemia</i>. (Thesis). University of Cape Town ,Faculty of Health Sciences ,Division of Clinical Haematology. Retrieved from http://hdl.handle.net/11427/6558en_ZA
dc.identifier.chicagocitationDu Pisani, Louis Almero. <i>"The development of a flow cytometric method to detect the presence of mutated nucleophosmin in Acute Myeloid Leukaemia."</i> Thesis., University of Cape Town ,Faculty of Health Sciences ,Division of Clinical Haematology, 2014. http://hdl.handle.net/11427/6558en_ZA
dc.identifier.citationDu Pisani, L. 2014. The development of a flow cytometric method to detect the presence of mutated nucleophosmin in Acute Myeloid Leukaemia. University of Cape Town.en_ZA
dc.identifier.ris TY - Thesis / Dissertation AU - Du Pisani, Louis Almero AB - Nucleophosmin (NPM1) is an acidic, nucleo-cytoplasmic, shuttling protein with predominant nucleolar localisation that plays multiple roles in cell growth and proliferation. Deletion insertion mutations of NPM1 (NPM1 DIM) seem to disrupt it normal physiologic role as a molecular chaperone, which likely leads to its oncogenic potential.NPM1 if present alone (not associated with FLT3 internal tandem duplications (FLT3-ITD)) is associated with significantly better overall survival and disease free survival in AML and has been entered as a provisional category in the World Health Organisation (2008) classification of Acute Myeloid Leukaemia with recurrent genetic abnormalities. Current methodology uses reverse transcriptase polymerase chain reaction (RT-PCR) and genomic deoxyribonucleic acid (DNA) PCR techniques to detect NPM1 DIM. Although these methods are robust and relatively easy to perform they can be expensive, labour intensive and not universally available. Six major variants of NPM1 DIM (Types A-F) have been described all leading to frame shift. All six types share the same last five amino acids in the C-terminal.The aim of this study was to develop a robust flow cytometry methodology that could be used in the routine assessment of AML samples to determine the mutational state of NPM, using a commercially available polyclonal antibody against the mutated NPM1. DA - 2014 DB - OpenUCT DP - University of Cape Town LK - https://open.uct.ac.za PB - University of Cape Town PY - 2014 T1 - The development of a flow cytometric method to detect the presence of mutated nucleophosmin in Acute Myeloid Leukaemia TI - The development of a flow cytometric method to detect the presence of mutated nucleophosmin in Acute Myeloid Leukaemia UR - http://hdl.handle.net/11427/6558 ER - en_ZA
dc.identifier.urihttp://hdl.handle.net/11427/6558
dc.identifier.vancouvercitationDu Pisani LA. The development of a flow cytometric method to detect the presence of mutated nucleophosmin in Acute Myeloid Leukaemia. [Thesis]. University of Cape Town ,Faculty of Health Sciences ,Division of Clinical Haematology, 2014 [cited yyyy month dd]. Available from: http://hdl.handle.net/11427/6558en_ZA
dc.language.isoengen_ZA
dc.publisher.departmentDivision of Clinical Haematologyen_ZA
dc.publisher.facultyFaculty of Health Sciencesen_ZA
dc.publisher.institutionUniversity of Cape Town
dc.titleThe development of a flow cytometric method to detect the presence of mutated nucleophosmin in Acute Myeloid Leukaemiaen_ZA
dc.typeMaster Thesis
dc.type.qualificationlevelMasters
dc.type.qualificationnameMMeden_ZA
uct.type.filetypeText
uct.type.filetypeImage
uct.type.publicationResearchen_ZA
uct.type.resourceThesisen_ZA
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