Study of anaerobic lactate metabolism under biosulphidogenic conditions
| dc.contributor.author | Oyekola, Oluwaseun A | |
| dc.contributor.author | van Hille, Robert P | |
| dc.contributor.author | Harrison, Susan T L | |
| dc.date.accessioned | 2016-08-18T12:43:05Z | |
| dc.date.available | 2016-08-18T12:43:05Z | |
| dc.date.issued | 2009 | |
| dc.date.updated | 2016-08-17T11:37:13Z | |
| dc.description.abstract | Biological sulfate reduction (BSR) has been reported to have potential for the treatment of acid mine drainage (AMD). The provision of a suitable carbon source and electron donor for this process remains a challenge. Lactate offers potential advantages as carbon source and electron donor in the biological sulfate reduction process. As this substrate is utilized by both fermentative bacteria and oxidative sulfate-reducing bacteria (SRB), the effect of feed sulfate concentration on the lactate pathways utilized under biosulfidogenic conditions was investigated. Studies were carried out in chemostat bioreactors across a range of residence times, using an enriched culture of SRB. The stoichiometry of biological sulfate reduction was affected by feed sulfate concentration and dilution rate. Incomplete oxidation of lactate was dominant at low feed sulfate concentration (1.0 g/L), while the yield of propionate from lactate metabolism increased at feed sulfate concentrations of 2.5–10.0 g/L, indicating the occurrence of lactate fermentation. Furthermore, at each sulfate feed concentration, in the range 2.5–10.0 g/L, the ratio in which lactate was metabolized by the oxidative and fermentative pathways varied with varying dilution rates. Lactate oxidation was higher at a feed sulfate concentration of 10.0 g/L relative to 2.5 and 5.0 g/L. The volumetric lactate utilization rate was enhanced by increasing the feed sulfate concentration. However, the proportion of total lactate consumed that was channelled into providing electrons for other activities apart from sulfate reduction also increased over the range of increasing sulfate concentrations studied and appeared to be a function of residual lactate and sulfide concentrations. | en_ZA |
| dc.identifier | http://dx.doi.org/10.1016/j.watres.2008.11.044 | |
| dc.identifier.apacitation | Oyekola, O. A., van Hille, R. P., & Harrison, S. T. L. (2009). Study of anaerobic lactate metabolism under biosulphidogenic conditions. <i>Water Research</i>, http://hdl.handle.net/11427/21320 | en_ZA |
| dc.identifier.chicagocitation | Oyekola, Oluwaseun A, Robert P van Hille, and Susan T L Harrison "Study of anaerobic lactate metabolism under biosulphidogenic conditions." <i>Water Research</i> (2009) http://hdl.handle.net/11427/21320 | en_ZA |
| dc.identifier.citation | Oyekola, O. O., Van Hille, R. P., & Harrison, S. T. (2009). Study of anaerobic lactate metabolism under biosulfidogenic conditions. Water research, 43(14), 3345-3354. | en_ZA |
| dc.identifier.issn | 0043-1354 | en_ZA |
| dc.identifier.ris | TY - Journal Article AU - Oyekola, Oluwaseun A AU - van Hille, Robert P AU - Harrison, Susan T L AB - Biological sulfate reduction (BSR) has been reported to have potential for the treatment of acid mine drainage (AMD). The provision of a suitable carbon source and electron donor for this process remains a challenge. Lactate offers potential advantages as carbon source and electron donor in the biological sulfate reduction process. As this substrate is utilized by both fermentative bacteria and oxidative sulfate-reducing bacteria (SRB), the effect of feed sulfate concentration on the lactate pathways utilized under biosulfidogenic conditions was investigated. Studies were carried out in chemostat bioreactors across a range of residence times, using an enriched culture of SRB. The stoichiometry of biological sulfate reduction was affected by feed sulfate concentration and dilution rate. Incomplete oxidation of lactate was dominant at low feed sulfate concentration (1.0 g/L), while the yield of propionate from lactate metabolism increased at feed sulfate concentrations of 2.5–10.0 g/L, indicating the occurrence of lactate fermentation. Furthermore, at each sulfate feed concentration, in the range 2.5–10.0 g/L, the ratio in which lactate was metabolized by the oxidative and fermentative pathways varied with varying dilution rates. Lactate oxidation was higher at a feed sulfate concentration of 10.0 g/L relative to 2.5 and 5.0 g/L. The volumetric lactate utilization rate was enhanced by increasing the feed sulfate concentration. However, the proportion of total lactate consumed that was channelled into providing electrons for other activities apart from sulfate reduction also increased over the range of increasing sulfate concentrations studied and appeared to be a function of residual lactate and sulfide concentrations. DA - 2009 DB - OpenUCT DP - University of Cape Town J1 - Water Research LK - https://open.uct.ac.za PB - University of Cape Town PY - 2009 SM - 0043-1354 T1 - Study of anaerobic lactate metabolism under biosulphidogenic conditions TI - Study of anaerobic lactate metabolism under biosulphidogenic conditions UR - http://hdl.handle.net/11427/21320 ER - | en_ZA |
| dc.identifier.uri | http://hdl.handle.net/11427/21320 | |
| dc.identifier.vancouvercitation | Oyekola OA, van Hille RP, Harrison STL. Study of anaerobic lactate metabolism under biosulphidogenic conditions. Water Research. 2009; http://hdl.handle.net/11427/21320. | en_ZA |
| dc.language | eng | en_ZA |
| dc.publisher | IWA Publishing | en_ZA |
| dc.publisher.institution | University of Cape Town | |
| dc.source | Water Research | en_ZA |
| dc.source.uri | http://www.sciencedirect.com/science/journal/00431354 | |
| dc.subject.other | Acid mine drainage | |
| dc.subject.other | Biological sulfate reduction | |
| dc.subject.other | Lactate oxidation | |
| dc.subject.other | Lactate fermentation | |
| dc.title | Study of anaerobic lactate metabolism under biosulphidogenic conditions | en_ZA |
| dc.type | Journal Article | en_ZA |
| uct.type.filetype | Text | |
| uct.type.filetype | Image | |
| uct.type.publication | Research | en_ZA |
| uct.type.resource | Article | en_ZA |