Longitudinal IgA and IgG Response, and ACE2 Binding Blockade, to Full-Length SARS-CoV-2 Spike Protein Variants in a Population of Black PLWH Vaccinated with ChAdOx1 nCoV-19

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dc.contributor.authorSmith, Muneerah
dc.contributor.authorKwatra, Gaurav
dc.contributor.authorIzu, Alane
dc.contributor.authorNel, Andrew
dc.contributor.authorCutland, Clare
dc.contributor.authorAhmed, Khatja
dc.contributor.authorBaillie, Vicky
dc.contributor.authorBarnabas, Shaun
dc.contributor.authorBhorat, Qasim
dc.contributor.authorBriner, Carmen
dc.contributor.authorLazarus, Erica
dc.contributor.authorDheda, Keertan
dc.contributor.authorFairlie, Lee
dc.contributor.authorKoen, Anthonet
dc.contributor.authorMadhi, Shabir
dc.contributor.authorBlackburn, Jonathan M.
dc.date.accessioned2024-04-29T10:31:05Z
dc.date.available2024-04-29T10:31:05Z
dc.date.issued2023-02-06
dc.date.updated2023-02-24T14:09:28Z
dc.description.abstractVaccines against SARS-CoV-2 have been pivotal in overcoming the COVID-19 pandemic yet understanding the subsequent outcomes and immunological effects remain crucial, especially for at-risk groups e.g., people living with human immunodeficiency virus (HIV) (PLWH). In this study we report the longitudinal IgA and IgG antibody titers, as well as antibody-mediated angiotensin converting enzyme 2 (ACE2) binding blockade, against the SARS-CoV-2 spike (S) proteins after 1 and 2 doses of the ChAdOx1 nCoV-19 vaccine in a population of Black PLWH. Here, we report that PLWH (N = 103) did not produce an anti-S IgA response after infection or vaccination, however, anti-S IgG was detected in response to vaccination and infection, with the highest level detected for infected vaccinated participants. The anti-IgG and ACE2 blockade assays revealed that both vaccination and infection resulted in IgG production, however, only vaccination resulted in a moderate increase in ACE2 binding blockade to the ancestral S protein. Vaccination with a previous infection results in the greatest anti-S IgG and ACE2 blockade for the ancestral S protein. In conclusion, PLWH produce an anti-S IgG response to the ChAdOx1 nCoV-19 vaccine and/or infection, and ChAdOx1 nCoV-19 vaccination with a previous infection produced more neutralizing antibodies than vaccination alone.
dc.identifierdoi: 10.3390/v15020448
dc.identifier.apacitationSmith, M., Kwatra, G., Izu, A., Nel, A., Cutland, C., Ahmed, K., ... Blackburn, Jonathan M. (2023). Longitudinal IgA and IgG Response, and ACE2 Binding Blockade, to Full-Length SARS-CoV-2 Spike Protein Variants in a Population of Black PLWH Vaccinated with ChAdOx1 nCoV-19. http://hdl.handle.net/11427/39488en_ZA
dc.identifier.chicagocitationSmith, Muneerah, Gaurav Kwatra, Alane Izu, Andrew Nel, Clare Cutland, Khatja Ahmed, Vicky Baillie, et al "Longitudinal IgA and IgG Response, and ACE2 Binding Blockade, to Full-Length SARS-CoV-2 Spike Protein Variants in a Population of Black PLWH Vaccinated with ChAdOx1 nCoV-19." (2023) http://hdl.handle.net/11427/39488en_ZA
dc.identifier.citationViruses 15 (2): 448 (2023)
dc.identifier.ris TY - Journal Article AU - Smith, Muneerah AU - Kwatra, Gaurav AU - Izu, Alane AU - Nel, Andrew AU - Cutland, Clare AU - Ahmed, Khatja AU - Baillie, Vicky AU - Barnabas, Shaun AU - Bhorat, Qasim AU - Briner, Carmen AU - Lazarus, Erica AU - Dheda, Keertan AU - Fairlie, Lee AU - Koen, Anthonet AU - Madhi, Shabir AU - Blackburn, Jonathan M. AB - Vaccines against SARS-CoV-2 have been pivotal in overcoming the COVID-19 pandemic yet understanding the subsequent outcomes and immunological effects remain crucial, especially for at-risk groups e.g., people living with human immunodeficiency virus (HIV) (PLWH). In this study we report the longitudinal IgA and IgG antibody titers, as well as antibody-mediated angiotensin converting enzyme 2 (ACE2) binding blockade, against the SARS-CoV-2 spike (S) proteins after 1 and 2 doses of the ChAdOx1 nCoV-19 vaccine in a population of Black PLWH. Here, we report that PLWH (N = 103) did not produce an anti-S IgA response after infection or vaccination, however, anti-S IgG was detected in response to vaccination and infection, with the highest level detected for infected vaccinated participants. The anti-IgG and ACE2 blockade assays revealed that both vaccination and infection resulted in IgG production, however, only vaccination resulted in a moderate increase in ACE2 binding blockade to the ancestral S protein. Vaccination with a previous infection results in the greatest anti-S IgG and ACE2 blockade for the ancestral S protein. In conclusion, PLWH produce an anti-S IgG response to the ChAdOx1 nCoV-19 vaccine and/or infection, and ChAdOx1 nCoV-19 vaccination with a previous infection produced more neutralizing antibodies than vaccination alone. DA - 2023-02-06 DB - OpenUCT DP - University of Cape Town LK - https://open.uct.ac.za PY - 2023 T1 - Longitudinal IgA and IgG Response, and ACE2 Binding Blockade, to Full-Length SARS-CoV-2 Spike Protein Variants in a Population of Black PLWH Vaccinated with ChAdOx1 nCoV-19 TI - Longitudinal IgA and IgG Response, and ACE2 Binding Blockade, to Full-Length SARS-CoV-2 Spike Protein Variants in a Population of Black PLWH Vaccinated with ChAdOx1 nCoV-19 UR - http://hdl.handle.net/11427/39488 ER - en_ZA
dc.identifier.urihttp://hdl.handle.net/11427/39488
dc.identifier.vancouvercitationSmith M, Kwatra G, Izu A, Nel A, Cutland C, Ahmed K, et al. Longitudinal IgA and IgG Response, and ACE2 Binding Blockade, to Full-Length SARS-CoV-2 Spike Protein Variants in a Population of Black PLWH Vaccinated with ChAdOx1 nCoV-19. 2023; http://hdl.handle.net/11427/39488.en_ZA
dc.titleLongitudinal IgA and IgG Response, and ACE2 Binding Blockade, to Full-Length SARS-CoV-2 Spike Protein Variants in a Population of Black PLWH Vaccinated with ChAdOx1 nCoV-19
dc.typeJournal Article
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