A comparative analysis of polyfunctional T cells and secreted cytokines induced by Bacille Calmette-Guerin immunisation in children and adults

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dc.contributor.authorRitz, Nicoleen_ZA
dc.contributor.authorStrach, Madeleineen_ZA
dc.contributor.authorYau, Carmenen_ZA
dc.contributor.authorDutta, Binitaen_ZA
dc.contributor.authorTebruegge, Marcen_ZA
dc.contributor.authorConnell, Tom Gen_ZA
dc.contributor.authorHanekom, Willem Aen_ZA
dc.contributor.authorBritton, Warwick Jen_ZA
dc.contributor.authorRobins-Browne, Royen_ZA
dc.contributor.authorCurtis, Nigelen_ZA
dc.date.accessioned2016-01-11T06:52:11Z
dc.date.available2016-01-11T06:52:11Z
dc.date.issued2012en_ZA
dc.description.abstractBCG vaccine is one of the most commonly-administered vaccines worldwide. Studies suggest the protective efficacy of BCG against TB is better for children than for adults. One potential explanation is that BCG induces a better protective immune response in children. Twenty six children and adults were immunised with BCG. The proportion of Th1-cytokine-producing mycobacterial-specific T cells, and the concentrations of secreted cytokines, were measured before and 10 weeks after BCG immunisation. A significant increase in the proportion of mycobacterial-specific cytokine-producing T cells was observed in both age groups. After BCG immunisation, children and adults had comparable proportions of mycobacterial-specific polyfunctional CD4 T cells when measured relative to the total number of CD4 T cells. However, relative to the subset of Th-1-cytokine-producing CD4 T cells, the proportion of polyfunctional cells was greater in children. Concentrations of secreted cytokines were comparable in children and adults. These findings suggest that the mycobacterial-specific cell-mediated immune response induced by BCG immunisation in children and adults is similar. The implication of a shift to a more polyfunctional immune response within the Th1-cytokine-producing CD4 T cells in children is uncertain as this aspect of the immune response has not been assessed as a potential correlate of protection against TB.en_ZA
dc.identifier.apacitationRitz, N., Strach, M., Yau, C., Dutta, B., Tebruegge, M., Connell, T. G., ... Curtis, N. (2012). A comparative analysis of polyfunctional T cells and secreted cytokines induced by Bacille Calmette-Guerin immunisation in children and adults. <i>PLoS One</i>, http://hdl.handle.net/11427/16258en_ZA
dc.identifier.chicagocitationRitz, Nicole, Madeleine Strach, Carmen Yau, Binita Dutta, Marc Tebruegge, Tom G Connell, Willem A Hanekom, Warwick J Britton, Roy Robins-Browne, and Nigel Curtis "A comparative analysis of polyfunctional T cells and secreted cytokines induced by Bacille Calmette-Guerin immunisation in children and adults." <i>PLoS One</i> (2012) http://hdl.handle.net/11427/16258en_ZA
dc.identifier.citationRitz, N., Strach, M., Yau, C., Dutta, B., Tebruegge, M., Connell, T. G., ... & Curtis, N. (2012). A comparative analysis of polyfunctional T cells and secreted cytokines induced by Bacille Calmette-Guerin immunisation in children and adults. PloS one, 7(7), e37535. doi:10.1371/journal.pone.0037535en_ZA
dc.identifier.ris TY - Journal Article AU - Ritz, Nicole AU - Strach, Madeleine AU - Yau, Carmen AU - Dutta, Binita AU - Tebruegge, Marc AU - Connell, Tom G AU - Hanekom, Willem A AU - Britton, Warwick J AU - Robins-Browne, Roy AU - Curtis, Nigel AB - BCG vaccine is one of the most commonly-administered vaccines worldwide. Studies suggest the protective efficacy of BCG against TB is better for children than for adults. One potential explanation is that BCG induces a better protective immune response in children. Twenty six children and adults were immunised with BCG. The proportion of Th1-cytokine-producing mycobacterial-specific T cells, and the concentrations of secreted cytokines, were measured before and 10 weeks after BCG immunisation. A significant increase in the proportion of mycobacterial-specific cytokine-producing T cells was observed in both age groups. After BCG immunisation, children and adults had comparable proportions of mycobacterial-specific polyfunctional CD4 T cells when measured relative to the total number of CD4 T cells. However, relative to the subset of Th-1-cytokine-producing CD4 T cells, the proportion of polyfunctional cells was greater in children. Concentrations of secreted cytokines were comparable in children and adults. These findings suggest that the mycobacterial-specific cell-mediated immune response induced by BCG immunisation in children and adults is similar. The implication of a shift to a more polyfunctional immune response within the Th1-cytokine-producing CD4 T cells in children is uncertain as this aspect of the immune response has not been assessed as a potential correlate of protection against TB. DA - 2012 DB - OpenUCT DO - 10.1371/journal.pone.0037535 DP - University of Cape Town J1 - PLoS One LK - https://open.uct.ac.za PB - University of Cape Town PY - 2012 T1 - A comparative analysis of polyfunctional T cells and secreted cytokines induced by Bacille Calmette-Guerin immunisation in children and adults TI - A comparative analysis of polyfunctional T cells and secreted cytokines induced by Bacille Calmette-Guerin immunisation in children and adults UR - http://hdl.handle.net/11427/16258 ER - en_ZA
dc.identifier.urihttp://hdl.handle.net/11427/16258
dc.identifier.urihttp://dx.doi.org/10.1371/journal.pone.0037535
dc.identifier.vancouvercitationRitz N, Strach M, Yau C, Dutta B, Tebruegge M, Connell TG, et al. A comparative analysis of polyfunctional T cells and secreted cytokines induced by Bacille Calmette-Guerin immunisation in children and adults. PLoS One. 2012; http://hdl.handle.net/11427/16258.en_ZA
dc.language.isoengen_ZA
dc.publisherPublic Library of Scienceen_ZA
dc.publisher.departmentSouth African Tuberculosis Vaccine Initiative (SATVI)en_ZA
dc.publisher.facultyFaculty of Health Sciencesen_ZA
dc.publisher.institutionUniversity of Cape Town
dc.rightscredited.en_ZA
dc.rights.holder© 2012 Ritz et alen_ZA
dc.rights.urihttp://creativecommons.org/licenses/by/4.0en_ZA
dc.sourcePLoS Oneen_ZA
dc.source.urihttp://journals.plos.org/plosoneen_ZA
dc.subject.otherT cellsen_ZA
dc.subject.otherT helper cellsen_ZA
dc.subject.otherCytokinesen_ZA
dc.subject.otherImmune responseen_ZA
dc.subject.otherChildrenen_ZA
dc.subject.otherAdultsen_ZA
dc.subject.otherCytotoxic T cellsen_ZA
dc.subject.otherTuberculosisen_ZA
dc.titleA comparative analysis of polyfunctional T cells and secreted cytokines induced by Bacille Calmette-Guerin immunisation in children and adultsen_ZA
dc.typeJournal Articleen_ZA
uct.type.filetypeText
uct.type.filetypeImage
uct.type.publicationResearchen_ZA
uct.type.resourceArticleen_ZA
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