The analysis of marine algae photosynthetic pigments by high performance liquid chromatography

dc.contributor.advisorOrren, M Jen_ZA
dc.contributor.authorBacon, Elizabeth Janeen_ZA
dc.date.accessioned2016-02-15T07:16:06Z
dc.date.available2016-02-15T07:16:06Z
dc.date.issued1984en_ZA
dc.descriptionBibliography: pages 157-165.en_ZA
dc.description.abstractA method of analysis of the photosynthetic pigments of in vitro algal cultures, using reverse-phase high performance liquid chromatography (HPLC) is described. This technique was extended to analysis of photosynthetic pigments in samples from the SIBEX-I Expedition; a preconcentration technique was developed to allow a reduction in sample volume for waters of low productivity. HPLC analysis allows accurate chlorophyll a determinations to be made in the presence of the spectrally similar chlorophyllide a, which has been found to be the major chlorophyll a breakdown product present in natural waters. Comparative studies with the SCOR-UNESCO method have shown that as a result of chlorophyllide interference the chlorophyll a concentrations are in fact over-estimates to varying degrees depending on the productivity of the biomass. In addition, quantification of chlorophylls b and c and the carotenoid complement allows identification of the major algal species present, and provides an indication of the physiological status of the bloom.en_ZA
dc.identifier.apacitationBacon, E. J. (1984). <i>The analysis of marine algae photosynthetic pigments by high performance liquid chromatography</i>. (Thesis). University of Cape Town ,Faculty of Science ,Department of Chemistry. Retrieved from http://hdl.handle.net/11427/17047en_ZA
dc.identifier.chicagocitationBacon, Elizabeth Jane. <i>"The analysis of marine algae photosynthetic pigments by high performance liquid chromatography."</i> Thesis., University of Cape Town ,Faculty of Science ,Department of Chemistry, 1984. http://hdl.handle.net/11427/17047en_ZA
dc.identifier.citationBacon, E. 1984. The analysis of marine algae photosynthetic pigments by high performance liquid chromatography. University of Cape Town.en_ZA
dc.identifier.ris TY - Thesis / Dissertation AU - Bacon, Elizabeth Jane AB - A method of analysis of the photosynthetic pigments of in vitro algal cultures, using reverse-phase high performance liquid chromatography (HPLC) is described. This technique was extended to analysis of photosynthetic pigments in samples from the SIBEX-I Expedition; a preconcentration technique was developed to allow a reduction in sample volume for waters of low productivity. HPLC analysis allows accurate chlorophyll a determinations to be made in the presence of the spectrally similar chlorophyllide a, which has been found to be the major chlorophyll a breakdown product present in natural waters. Comparative studies with the SCOR-UNESCO method have shown that as a result of chlorophyllide interference the chlorophyll a concentrations are in fact over-estimates to varying degrees depending on the productivity of the biomass. In addition, quantification of chlorophylls b and c and the carotenoid complement allows identification of the major algal species present, and provides an indication of the physiological status of the bloom. DA - 1984 DB - OpenUCT DP - University of Cape Town LK - https://open.uct.ac.za PB - University of Cape Town PY - 1984 T1 - The analysis of marine algae photosynthetic pigments by high performance liquid chromatography TI - The analysis of marine algae photosynthetic pigments by high performance liquid chromatography UR - http://hdl.handle.net/11427/17047 ER - en_ZA
dc.identifier.urihttp://hdl.handle.net/11427/17047
dc.identifier.vancouvercitationBacon EJ. The analysis of marine algae photosynthetic pigments by high performance liquid chromatography. [Thesis]. University of Cape Town ,Faculty of Science ,Department of Chemistry, 1984 [cited yyyy month dd]. Available from: http://hdl.handle.net/11427/17047en_ZA
dc.language.isoengen_ZA
dc.publisher.departmentDepartment of Chemistryen_ZA
dc.publisher.facultyFaculty of Scienceen_ZA
dc.publisher.institutionUniversity of Cape Town
dc.subject.otherAnalytical Scienceen_ZA
dc.titleThe analysis of marine algae photosynthetic pigments by high performance liquid chromatographyen_ZA
dc.typeMaster Thesis
dc.type.qualificationlevelMasters
dc.type.qualificationnameMScen_ZA
uct.type.filetypeText
uct.type.filetypeImage
uct.type.publicationResearchen_ZA
uct.type.resourceThesisen_ZA
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