Generation of Liposomes to Study the Effect of Mycobacterium Tuberculosis Lipids on HIV-1 cis- and trans-Infections

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dc.contributor.authorPouget, Marion
dc.contributor.authorCoussens, Anna K.
dc.contributor.authorRuggiero, Alessandra
dc.contributor.authorKoch, Anastasia
dc.contributor.authorThomas, Jordan
dc.contributor.authorBesra, Gurdyal S.
dc.contributor.authorWilkinson, Robert J.
dc.contributor.authorBhatt, Apoorva
dc.contributor.authorPollakis, Georgios
dc.contributor.authorPaxton, William A.
dc.date.accessioned2021-10-19T15:24:50Z
dc.date.available2021-10-19T15:24:50Z
dc.date.issued2021-02-16
dc.date.updated2021-02-26T14:54:16Z
dc.description.abstractTuberculosis (TB) is the leading cause of death among HIV-1-infected individuals and Mycobacterium tuberculosis (Mtb) co-infection is an early precipitate to AIDS. We aimed to determine whether Mtb strains differentially modulate cellular susceptibility to HIV-1 infection (cis- and trans-infection), via surface receptor interaction by their cell envelope lipids. Total lipids from pathogenic (lineage 4 Mtb H37Rv, CDC1551 and lineage 2 Mtb HN878, EU127) and non-pathogenic (Mycobacterium bovis BCG and Mycobacterium smegmatis) Mycobacterium strains were integrated into liposomes mimicking the lipid distribution and antigen accessibility of the mycobacterial cell wall. The resulting liposomes were tested for modulating in vitro HIV-1 cis- and trans-infection of TZM-bl cells using single-cycle infectious virus particles. Mtb glycolipids did not affect HIV-1 direct infection however, trans-infection of both R5 and X4 tropic HIV-1 strains were impaired in the presence of glycolipids from M. bovis, Mtb H37Rv and Mtb EU127 strains when using Raji-DC-SIGN cells or immature and mature dendritic cells (DCs) to capture virus. SL1, PDIM and TDM lipids were identified to be involved in DC-SIGN recognition and impairment of HIV-1 trans-infection. These findings indicate that variant strains of Mtb have differential effect on HIV-1 trans-infection with the potential to influence HIV-1 disease course in co-infected individuals.en_US
dc.identifierdoi: 10.3390/ijms22041945
dc.identifier.apacitationPouget, M., Coussens, Anna K., Ruggiero, A., Koch, A., Thomas, J., Besra, Gurdyal S., ... Paxton, William A. (2021). Generation of Liposomes to Study the Effect of Mycobacterium Tuberculosis Lipids on HIV-1 cis- and trans-Infections. <i>International Journal of Molecular Sciences</i>, 22(4), http://hdl.handle.net/11427/35273en_ZA
dc.identifier.chicagocitationPouget, Marion, Anna K. Coussens, Alessandra Ruggiero, Anastasia Koch, Jordan Thomas, Gurdyal S. Besra, Robert J. Wilkinson, Apoorva Bhatt, Georgios Pollakis, and William A. Paxton "Generation of Liposomes to Study the Effect of Mycobacterium Tuberculosis Lipids on HIV-1 cis- and trans-Infections." <i>International Journal of Molecular Sciences</i> 22, 4. (2021) http://hdl.handle.net/11427/35273en_ZA
dc.identifier.citationPouget, M., Coussens, Anna K., Ruggiero, A., Koch, A., Thomas, J., Besra, Gurdyal S., Wilkinson, Robert J. & Bhatt, A. et al. 2021. Generation of Liposomes to Study the Effect of Mycobacterium Tuberculosis Lipids on HIV-1 cis- and trans-Infections. <i>International Journal of Molecular Sciences.</i> 22(4) http://hdl.handle.net/11427/35273en_ZA
dc.identifier.ris TY - Journal Article AU - Pouget, Marion AU - Coussens, Anna K. AU - Ruggiero, Alessandra AU - Koch, Anastasia AU - Thomas, Jordan AU - Besra, Gurdyal S. AU - Wilkinson, Robert J. AU - Bhatt, Apoorva AU - Pollakis, Georgios AU - Paxton, William A. AB - Tuberculosis (TB) is the leading cause of death among HIV-1-infected individuals and Mycobacterium tuberculosis (Mtb) co-infection is an early precipitate to AIDS. We aimed to determine whether Mtb strains differentially modulate cellular susceptibility to HIV-1 infection (cis- and trans-infection), via surface receptor interaction by their cell envelope lipids. Total lipids from pathogenic (lineage 4 Mtb H37Rv, CDC1551 and lineage 2 Mtb HN878, EU127) and non-pathogenic (Mycobacterium bovis BCG and Mycobacterium smegmatis) Mycobacterium strains were integrated into liposomes mimicking the lipid distribution and antigen accessibility of the mycobacterial cell wall. The resulting liposomes were tested for modulating in vitro HIV-1 cis- and trans-infection of TZM-bl cells using single-cycle infectious virus particles. Mtb glycolipids did not affect HIV-1 direct infection however, trans-infection of both R5 and X4 tropic HIV-1 strains were impaired in the presence of glycolipids from M. bovis, Mtb H37Rv and Mtb EU127 strains when using Raji-DC-SIGN cells or immature and mature dendritic cells (DCs) to capture virus. SL1, PDIM and TDM lipids were identified to be involved in DC-SIGN recognition and impairment of HIV-1 trans-infection. These findings indicate that variant strains of Mtb have differential effect on HIV-1 trans-infection with the potential to influence HIV-1 disease course in co-infected individuals. DA - 2021-02-16 DB - OpenUCT DP - University of Cape Town IS - 4 J1 - International Journal of Molecular Sciences KW - HIV-1 KW - TB KW - Mycobacterium tuberculosis KW - in vitro KW - DC-SIGN KW - liposomes KW - trans-infection KW - SL1 KW - TDM KW - PDIM KW - H37Rv KW - HN878 KW - CDC1551 KW - EU127 KW - BCG KW - M. smegmatis LK - https://open.uct.ac.za PY - 2021 T1 - Generation of Liposomes to Study the Effect of Mycobacterium Tuberculosis Lipids on HIV-1 cis- and trans-Infections TI - Generation of Liposomes to Study the Effect of Mycobacterium Tuberculosis Lipids on HIV-1 cis- and trans-Infections UR - http://hdl.handle.net/11427/35273 ER - en_ZA
dc.identifier.urihttps://doi.org/10.3390/ijms22041945
dc.identifier.urihttp://hdl.handle.net/11427/35273
dc.identifier.vancouvercitationPouget M, Coussens Anna K, Ruggiero A, Koch A, Thomas J, Besra Gurdyal S, et al. Generation of Liposomes to Study the Effect of Mycobacterium Tuberculosis Lipids on HIV-1 cis- and trans-Infections. International Journal of Molecular Sciences. 2021;22(4) http://hdl.handle.net/11427/35273.en_ZA
dc.language.isoenen_US
dc.publisher.departmentDepartment of Medicineen_US
dc.publisher.facultyFaculty of Health Sciencesen_US
dc.rights.urihttp://creativecommons.org/licenses/by/4.0/en_US
dc.sourceInternational Journal of Molecular Sciencesen_US
dc.source.journalissue4en_US
dc.source.journalvolume22en_US
dc.source.urihttps://www.mdpi.com/journal/ijms
dc.subjectHIV-1
dc.subjectTB
dc.subjectMycobacterium tuberculosis
dc.subjectin vitro
dc.subjectDC-SIGN
dc.subjectliposomes
dc.subjecttrans-infection
dc.subjectSL1
dc.subjectTDM
dc.subjectPDIM
dc.subjectH37Rv
dc.subjectHN878
dc.subjectCDC1551
dc.subjectEU127
dc.subjectBCG
dc.subjectM. smegmatis
dc.titleGeneration of Liposomes to Study the Effect of Mycobacterium Tuberculosis Lipids on HIV-1 cis- and trans-Infectionsen_US
dc.typeJournal Articleen_US
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