Subcellular organization of N2-fixing nodules of cowpea (Vigna unguiculata) supplied with silicon.
| dc.contributor.author | Nelwamondo, Azwianewi | |
| dc.contributor.author | Jaffer, Mohamed A | |
| dc.contributor.author | Dakora, Felix D | |
| dc.date.accessioned | 2016-08-02T13:59:06Z | |
| dc.date.available | 2016-08-02T13:59:06Z | |
| dc.date.issued | 2001 | |
| dc.date.updated | 2016-08-02T09:15:20Z | |
| dc.description.abstract | Provision of silicon (0, 0.048, 0.096, 0.24, 0.48, and 0.96 g/1) in the form of silicic acid (H4SiO4) to nodulated cowpea plants(Vignia unguiculata [L.] Walp.) grown in liquid culture resulted in considerable changes in the internal organization of nodule structure. Compared to the control plants which received no added silicate, bacteroid numbers increased significantly (P ≤ 0.05) at silicate concentrations of both 0.096 and 0.48 g/1. The number of symbiosomes also increased by 3.2-fold at the silicate concentration of 0.96 g/1 compared to the control. In contrast, the size of bacteroids and symbiosomes decreased significantly (P ≤ 0.05) inside nodules of silicate-treated plants. The peribacteroid space was also decreased considerably (P ≤ 0.05) with the application of 0.096 and 0.96 g of silicate per liter to plants. However, the size of intercellular spaces adjacent to infected and uninfected interstitial cells within the nodule medulla increased significantly (P ≤ 0.05) at 0.096 g of silicate per liter followed by a sharply marked (P ≤ 0.05) decrease with each subsequent increase in silicate application. The result was a large decrease (P≤0.05) in the area of bacteria-infected tissue occupied by intercellular space at the highest silicate concentration, which was caused by a significant (P ≤ 0.05) increase in cell wall thickness. Our findings show that the positive effects of silicon on N2 fixation might actually be due to an increased number of bacteroids and symbiosomes. | en_ZA |
| dc.identifier | http://dx.doi.org/10.1007/BF02680136 | |
| dc.identifier.apacitation | Nelwamondo, A., Jaffer, M. A., & Dakora, F. D. (2001). Subcellular organization of N2-fixing nodules of cowpea (Vigna unguiculata) supplied with silicon. <i>Protoplasma</i>, http://hdl.handle.net/11427/21103 | en_ZA |
| dc.identifier.chicagocitation | Nelwamondo, Azwianewi, Mohamed A Jaffer, and Felix D Dakora "Subcellular organization of N2-fixing nodules of cowpea (Vigna unguiculata) supplied with silicon." <i>Protoplasma</i> (2001) http://hdl.handle.net/11427/21103 | en_ZA |
| dc.identifier.citation | Nelwamondo, A., Jaffer, M. A., & Dakora, F. D. (2001). Subcellular organization of N2-fixing nodules of cowpea (Vigna unguiculata) supplied with silicon. Protoplasma, 216(1-2), 94-100. | en_ZA |
| dc.identifier.issn | 0033-183X | en_ZA |
| dc.identifier.ris | TY - Journal Article AU - Nelwamondo, Azwianewi AU - Jaffer, Mohamed A AU - Dakora, Felix D AB - Provision of silicon (0, 0.048, 0.096, 0.24, 0.48, and 0.96 g/1) in the form of silicic acid (H4SiO4) to nodulated cowpea plants(Vignia unguiculata [L.] Walp.) grown in liquid culture resulted in considerable changes in the internal organization of nodule structure. Compared to the control plants which received no added silicate, bacteroid numbers increased significantly (P ≤ 0.05) at silicate concentrations of both 0.096 and 0.48 g/1. The number of symbiosomes also increased by 3.2-fold at the silicate concentration of 0.96 g/1 compared to the control. In contrast, the size of bacteroids and symbiosomes decreased significantly (P ≤ 0.05) inside nodules of silicate-treated plants. The peribacteroid space was also decreased considerably (P ≤ 0.05) with the application of 0.096 and 0.96 g of silicate per liter to plants. However, the size of intercellular spaces adjacent to infected and uninfected interstitial cells within the nodule medulla increased significantly (P ≤ 0.05) at 0.096 g of silicate per liter followed by a sharply marked (P ≤ 0.05) decrease with each subsequent increase in silicate application. The result was a large decrease (P≤0.05) in the area of bacteria-infected tissue occupied by intercellular space at the highest silicate concentration, which was caused by a significant (P ≤ 0.05) increase in cell wall thickness. Our findings show that the positive effects of silicon on N2 fixation might actually be due to an increased number of bacteroids and symbiosomes. DA - 2001 DB - OpenUCT DP - University of Cape Town J1 - Protoplasma LK - https://open.uct.ac.za PB - University of Cape Town PY - 2001 SM - 0033-183X T1 - Subcellular organization of N2-fixing nodules of cowpea (Vigna unguiculata) supplied with silicon TI - Subcellular organization of N2-fixing nodules of cowpea (Vigna unguiculata) supplied with silicon UR - http://hdl.handle.net/11427/21103 ER - | en_ZA |
| dc.identifier.uri | http://hdl.handle.net/11427/21103 | |
| dc.identifier.vancouvercitation | Nelwamondo A, Jaffer MA, Dakora FD. Subcellular organization of N2-fixing nodules of cowpea (Vigna unguiculata) supplied with silicon. Protoplasma. 2001; http://hdl.handle.net/11427/21103. | en_ZA |
| dc.language | eng | en_ZA |
| dc.publisher | Springer | en_ZA |
| dc.publisher.institution | University of Cape Town | |
| dc.source | Protoplasma | en_ZA |
| dc.source.uri | http://link.springer.com/journal/709 | |
| dc.subject.other | Silicon nutrition | |
| dc.subject.other | Cowpea | |
| dc.subject.other | Nodule | |
| dc.subject.other | Bacteroid | |
| dc.subject.other | Intercellular space | |
| dc.title | Subcellular organization of N2-fixing nodules of cowpea (Vigna unguiculata) supplied with silicon. | en_ZA |
| dc.type | Journal Article | en_ZA |
| uct.type.filetype | Text | |
| uct.type.filetype | Image | |
| uct.type.publication | Research | en_ZA |
| uct.type.resource | Article | en_ZA |