ERF5 and ERF6 play redundant roles as positive regulators of JA/Et-mediated defense against Botrytis cinerea in Arabidopsis

Simple item page
dc.contributor.authorMoffat, Caroline Sen_ZA
dc.contributor.authorIngle, Robert Aen_ZA
dc.contributor.authorWathugala, Deepthi Len_ZA
dc.contributor.authorSaunders, Nigel Jen_ZA
dc.contributor.authorKnight, Heatheren_ZA
dc.contributor.authorKnight, Marc Ren_ZA
dc.date.accessioned2015-11-23T12:32:11Z
dc.date.available2015-11-23T12:32:11Z
dc.date.issued2012en_ZA
dc.description.abstractThe ethylene response factor (ERF) family in Arabidopsis thaliana comprises 122 members in 12 groups, yet the biological functions of the majority remain unknown. Of the group IX ERFs, the IXc subgroup has been studied the most, and includes ERF1, ERF14 and ORA59, which play roles in plant innate immunity. Here we investigate the biological functions of two members of the less studied IXb subgroup: ERF5 and ERF6. In order to identify potential targets of these transcription factors, microarray analyses were performed on plants constitutively expressing either ERF5 or ERF6 . Expression of defense genes, JA/Et-responsive genes and genes containing the GCC box promoter motif were significantly upregulated in both ERF5 and ERF6 transgenic plants, suggesting that ERF5 and ERF6 may act as positive regulators of JA-mediated defense and potentially overlap in their function. Since defense against necrotrophic pathogens is generally mediated through JA/Et-signalling, resistance against the fungal necrotroph Botrytis cinerea was examined. Constitutive expression of ERF5 or ERF6 resulted in significantly increased resistance. Although no significant difference in susceptibility to B. cinerea was observed in either erf5 or erf6 mutants, the erf5 erf6 double mutant showed a significant increase in susceptibility, which was likely due to compromised JA-mediated gene expression, since JA-induced gene expression was reduced in the double mutant. Taken together these data suggest that ERF5 and ERF6 play positive but redundant roles in defense against B. cinerea . Since mutual antagonism between JA/Et and salicylic acid (SA) signalling is well known, the UV-C inducibility of an SA-inducible gene, PR-1 , was examined. Reduced inducibilty in both ERF5 and ERF6 constitutive overexepressors was consistent with suppression of SA-mediated signalling, as was an increased susceptibility to avirulent Pseudomonas syringae . These data suggest that ERF5 and ERF6 may also play a role in the antagonistic crosstalk between the JA/Et and SA signalling pathways.en_ZA
dc.identifier.apacitationMoffat, C. S., Ingle, R. A., Wathugala, D. L., Saunders, N. J., Knight, H., & Knight, M. R. (2012). ERF5 and ERF6 play redundant roles as positive regulators of JA/Et-mediated defense against Botrytis cinerea in Arabidopsis. <i>PLoS One</i>, http://hdl.handle.net/11427/15313en_ZA
dc.identifier.chicagocitationMoffat, Caroline S, Robert A Ingle, Deepthi L Wathugala, Nigel J Saunders, Heather Knight, and Marc R Knight "ERF5 and ERF6 play redundant roles as positive regulators of JA/Et-mediated defense against Botrytis cinerea in Arabidopsis." <i>PLoS One</i> (2012) http://hdl.handle.net/11427/15313en_ZA
dc.identifier.citationMoffat, C. S., Ingle, R. A., Wathugala, D. L., Saunders, N. J., Knight, H., & Knight, M. R. (2012). ERF5 and ERF6 play redundant roles as positive regulators of JA/Et-mediated defense against Botrytis cinerea in Arabidopsis. PLoS One, 7(4), e35995. doi:10.1371/journal.pone.0035995en_ZA
dc.identifier.ris TY - Journal Article AU - Moffat, Caroline S AU - Ingle, Robert A AU - Wathugala, Deepthi L AU - Saunders, Nigel J AU - Knight, Heather AU - Knight, Marc R AB - The ethylene response factor (ERF) family in Arabidopsis thaliana comprises 122 members in 12 groups, yet the biological functions of the majority remain unknown. Of the group IX ERFs, the IXc subgroup has been studied the most, and includes ERF1, ERF14 and ORA59, which play roles in plant innate immunity. Here we investigate the biological functions of two members of the less studied IXb subgroup: ERF5 and ERF6. In order to identify potential targets of these transcription factors, microarray analyses were performed on plants constitutively expressing either ERF5 or ERF6 . Expression of defense genes, JA/Et-responsive genes and genes containing the GCC box promoter motif were significantly upregulated in both ERF5 and ERF6 transgenic plants, suggesting that ERF5 and ERF6 may act as positive regulators of JA-mediated defense and potentially overlap in their function. Since defense against necrotrophic pathogens is generally mediated through JA/Et-signalling, resistance against the fungal necrotroph Botrytis cinerea was examined. Constitutive expression of ERF5 or ERF6 resulted in significantly increased resistance. Although no significant difference in susceptibility to B. cinerea was observed in either erf5 or erf6 mutants, the erf5 erf6 double mutant showed a significant increase in susceptibility, which was likely due to compromised JA-mediated gene expression, since JA-induced gene expression was reduced in the double mutant. Taken together these data suggest that ERF5 and ERF6 play positive but redundant roles in defense against B. cinerea . Since mutual antagonism between JA/Et and salicylic acid (SA) signalling is well known, the UV-C inducibility of an SA-inducible gene, PR-1 , was examined. Reduced inducibilty in both ERF5 and ERF6 constitutive overexepressors was consistent with suppression of SA-mediated signalling, as was an increased susceptibility to avirulent Pseudomonas syringae . These data suggest that ERF5 and ERF6 may also play a role in the antagonistic crosstalk between the JA/Et and SA signalling pathways. DA - 2012 DB - OpenUCT DO - 10.1371/journal.pone.0035995 DP - University of Cape Town J1 - PLoS One LK - https://open.uct.ac.za PB - University of Cape Town PY - 2012 T1 - ERF5 and ERF6 play redundant roles as positive regulators of JA/Et-mediated defense against Botrytis cinerea in Arabidopsis TI - ERF5 and ERF6 play redundant roles as positive regulators of JA/Et-mediated defense against Botrytis cinerea in Arabidopsis UR - http://hdl.handle.net/11427/15313 ER - en_ZA
dc.identifier.urihttp://hdl.handle.net/11427/15313
dc.identifier.urihttp://dx.doi.org/10.1371/journal.pone.0035995
dc.identifier.vancouvercitationMoffat CS, Ingle RA, Wathugala DL, Saunders NJ, Knight H, Knight MR. ERF5 and ERF6 play redundant roles as positive regulators of JA/Et-mediated defense against Botrytis cinerea in Arabidopsis. PLoS One. 2012; http://hdl.handle.net/11427/15313.en_ZA
dc.language.isoengen_ZA
dc.publisherPublic Library of Scienceen_ZA
dc.publisher.departmentDepartment of Molecular and Cell Biologyen_ZA
dc.publisher.facultyFaculty of Scienceen_ZA
dc.publisher.institutionUniversity of Cape Town
dc.rightsThis is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.en_ZA
dc.rights.holder© 2012 Moffat et alen_ZA
dc.rights.urihttp://creativecommons.org/licenses/by/4.0en_ZA
dc.sourcePLoS Oneen_ZA
dc.source.urihttp://journals.plos.org/plosoneen_ZA
dc.subject.otherJasmonic aciden_ZA
dc.subject.otherGene expressionen_ZA
dc.subject.otherMicroarraysen_ZA
dc.subject.otherPlant pathogensen_ZA
dc.subject.otherDefensinsen_ZA
dc.subject.otherGenetically modified plantsen_ZA
dc.subject.otherRNA extractionen_ZA
dc.subject.otherTranscription factorsen_ZA
dc.titleERF5 and ERF6 play redundant roles as positive regulators of JA/Et-mediated defense against Botrytis cinerea in Arabidopsisen_ZA
dc.typeJournal Articleen_ZA
uct.type.filetypeText
uct.type.filetypeImage
uct.type.publicationResearchen_ZA
uct.type.resourceArticleen_ZA
Files
Original bundle
Now showing 1 - 1 of 1
Thumbnail Image
Name:
Moffat_ERF5_and_ERF6_2012.pdf
Size:
344.87 KB
Format:
Adobe Portable Document Format
Description:
Download
Collections
Journal Articles