Randomized cross-sectional study to compare HIV-1 specific antibody and cytokine concentrations in female genital secretions obtained by menstrual cup and cervicovaginal lavage

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dc.contributor.authorArchary, Dersereeen_ZA
dc.contributor.authorLiebenberg, Lenine Jen_ZA
dc.contributor.authorWerner, Liseen_ZA
dc.contributor.authorTulsi, Sahilen_ZA
dc.contributor.authorMajola, Nelisileen_ZA
dc.contributor.authorNaicker, Nivashneeen_ZA
dc.contributor.authorDlamini, Sarahen_ZA
dc.contributor.authorHope, Thomas Jen_ZA
dc.contributor.authorSamsunder, Natashaen_ZA
dc.contributor.authorKarim, Salim S Abdoolen_ZA
dc.date.accessioned2016-01-02T05:05:47Z
dc.date.available2016-01-02T05:05:47Z
dc.date.issued2015en_ZA
dc.description.abstractIntroduction Optimizing methods for genital specimen collection to accurately characterize mucosal immune responses is a priority for the HIV prevention field. The menstrual cup (MC) has been proposed as an alternative to other methods including cervicovaginal lavage (CVL), but no study has yet formally compared these two methods. METHODS: Forty HIV-infected, antiretroviral therapy-naïve women from the CAPRISA 002 acute HIV infection cohort study were randomized to have genital fluid collected using the MC with subsequent CVL, or by CVL alone. Qualitative data, which assessed levels of comfort and acceptability of MC using a 5-point Likert scale, was collected. Luminex multiplex assays were used to measure HIV-specific IgG against multiple gene products and 48 cytokines. RESULTS: The majority (94%) of participants indicated that insertion, wearing and removal of the MC was comfortable. Nineteen MCs with 18 matching, subsequent CVLs and 20 randomized CVLs were available for analysis. Mucosal IgG responses against four HIV-antigens were detected in 99% of MCs compared to only 80% of randomized CVLs (p = 0.029). Higher specific antibody activity and total antibodies were observed in MCs compared to CVL (all p<0.001). In MCs, 42/48 (88%) cytokines were in the detectable range in all participants compared to 27/48 (54%) in CVL (p<0.001). Concentrations of 22/41 cytokines (53.7%) were significantly higher in fluid collected by MC. Both total IgG (r = 0.63; p = 0.005) and cytokine concentrations (r = 0.90; p<0.001) correlated strongly between MC and corresponding post-MC CVL. CONCLUSIONS: MC sampling improves the detection of mucosal cytokines and antibodies, particularly those present at low concentrations. MC may therefore represent an ideal tool to assess immunological parameters in genital secretions, without interfering with concurrent collection of conventional CVL samples.en_ZA
dc.identifier.apacitationArchary, D., Liebenberg, L. J., Werner, L., Tulsi, S., Majola, N., Naicker, N., ... Karim, S. S. A. (2015). Randomized cross-sectional study to compare HIV-1 specific antibody and cytokine concentrations in female genital secretions obtained by menstrual cup and cervicovaginal lavage. <i>PLoS One</i>, http://hdl.handle.net/11427/16162en_ZA
dc.identifier.chicagocitationArchary, Derseree, Lenine J Liebenberg, Lise Werner, Sahil Tulsi, Nelisile Majola, Nivashnee Naicker, Sarah Dlamini, Thomas J Hope, Natasha Samsunder, and Salim S Abdool Karim "Randomized cross-sectional study to compare HIV-1 specific antibody and cytokine concentrations in female genital secretions obtained by menstrual cup and cervicovaginal lavage." <i>PLoS One</i> (2015) http://hdl.handle.net/11427/16162en_ZA
dc.identifier.citationArchary, D., Liebenberg, L. J., Werner, L., Tulsi, S., Majola, N., Naicker, N., ... & Morris, L. (2015).Randomized cross-sectional study to compare HIV-1 specific antibody and cytokine concentrations in female genital secretions obtained by menstrual cup and cervicovaginal lavage. PloS one, 10(7). doi:10.1371/journal.pone.0131906en_ZA
dc.identifier.ris TY - Journal Article AU - Archary, Derseree AU - Liebenberg, Lenine J AU - Werner, Lise AU - Tulsi, Sahil AU - Majola, Nelisile AU - Naicker, Nivashnee AU - Dlamini, Sarah AU - Hope, Thomas J AU - Samsunder, Natasha AU - Karim, Salim S Abdool AB - Introduction Optimizing methods for genital specimen collection to accurately characterize mucosal immune responses is a priority for the HIV prevention field. The menstrual cup (MC) has been proposed as an alternative to other methods including cervicovaginal lavage (CVL), but no study has yet formally compared these two methods. METHODS: Forty HIV-infected, antiretroviral therapy-naïve women from the CAPRISA 002 acute HIV infection cohort study were randomized to have genital fluid collected using the MC with subsequent CVL, or by CVL alone. Qualitative data, which assessed levels of comfort and acceptability of MC using a 5-point Likert scale, was collected. Luminex multiplex assays were used to measure HIV-specific IgG against multiple gene products and 48 cytokines. RESULTS: The majority (94%) of participants indicated that insertion, wearing and removal of the MC was comfortable. Nineteen MCs with 18 matching, subsequent CVLs and 20 randomized CVLs were available for analysis. Mucosal IgG responses against four HIV-antigens were detected in 99% of MCs compared to only 80% of randomized CVLs (p = 0.029). Higher specific antibody activity and total antibodies were observed in MCs compared to CVL (all p<0.001). In MCs, 42/48 (88%) cytokines were in the detectable range in all participants compared to 27/48 (54%) in CVL (p<0.001). Concentrations of 22/41 cytokines (53.7%) were significantly higher in fluid collected by MC. Both total IgG (r = 0.63; p = 0.005) and cytokine concentrations (r = 0.90; p<0.001) correlated strongly between MC and corresponding post-MC CVL. CONCLUSIONS: MC sampling improves the detection of mucosal cytokines and antibodies, particularly those present at low concentrations. MC may therefore represent an ideal tool to assess immunological parameters in genital secretions, without interfering with concurrent collection of conventional CVL samples. DA - 2015 DB - OpenUCT DO - 10.1371/journal.pone.0131906 DP - University of Cape Town J1 - PLoS One LK - https://open.uct.ac.za PB - University of Cape Town PY - 2015 T1 - Randomized cross-sectional study to compare HIV-1 specific antibody and cytokine concentrations in female genital secretions obtained by menstrual cup and cervicovaginal lavage TI - Randomized cross-sectional study to compare HIV-1 specific antibody and cytokine concentrations in female genital secretions obtained by menstrual cup and cervicovaginal lavage UR - http://hdl.handle.net/11427/16162 ER - en_ZA
dc.identifier.urihttp://hdl.handle.net/11427/16162
dc.identifier.urihttp://dx.doi.org/10.1371/journal.pone.0131906
dc.identifier.vancouvercitationArchary D, Liebenberg LJ, Werner L, Tulsi S, Majola N, Naicker N, et al. Randomized cross-sectional study to compare HIV-1 specific antibody and cytokine concentrations in female genital secretions obtained by menstrual cup and cervicovaginal lavage. PLoS One. 2015; http://hdl.handle.net/11427/16162.en_ZA
dc.language.isoengen_ZA
dc.publisherPublic Library of Scienceen_ZA
dc.publisher.departmentInstitute of Infectious Disease and Molecular Medicineen_ZA
dc.publisher.facultyFaculty of Health Sciencesen_ZA
dc.publisher.institutionUniversity of Cape Town
dc.rightsThis is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.en_ZA
dc.rights.holder© 2015 Archary et alen_ZA
dc.rights.urihttp://creativecommons.org/licenses/by/4.0en_ZA
dc.sourcePLoS Oneen_ZA
dc.source.urihttp://journals.plos.org/plosoneen_ZA
dc.subject.otherCytokinesen_ZA
dc.subject.otherAntibodiesen_ZA
dc.subject.otherSecretionen_ZA
dc.subject.otherHIVen_ZA
dc.subject.otherHIV-1en_ZA
dc.subject.otherFemale contraceptionen_ZA
dc.subject.otherNerve growth factoren_ZA
dc.subject.otherHIV preventionen_ZA
dc.titleRandomized cross-sectional study to compare HIV-1 specific antibody and cytokine concentrations in female genital secretions obtained by menstrual cup and cervicovaginal lavageen_ZA
dc.typeJournal Articleen_ZA
uct.type.filetypeText
uct.type.filetypeImage
uct.type.publicationResearchen_ZA
uct.type.resourceArticleen_ZA
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