Identification of proteins that interact with brain factor-1 and characterization of these interactions

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2000

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University of Cape Town

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Brain Factor-1 (BF-1) is winged helix transcription factor that is essential for the development of the cerebral hemispheres and olfactory neuroepithelium. BF-1 is expressed in the telecephalic neuroepithelium, olfactory placode and the nasal half of the optic stalk and retina during embryogenesis (Hatini et al 1994; Lai and Tao 1992). In these tissues, BF-1 plays multiple roles in regional patterning and regulating cell proliferation and differentiation. In order to investigate how BF-1 controls these various processes, the aim of this thesis was to identify proteins which interact with BF-1 using the Yeast Two Hybrid System (Field and Song 1989) and to characterise these interactions.
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Bibliography: leaves 110-124.

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