Genetic analysis of maize streak virus isolates from Uganda reveals widespread distribution of a recombinant variant.

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dc.contributor.authorOwor, Betty E
dc.contributor.authorMartin, Darren P
dc.contributor.authorShepherd, Dionne N
dc.contributor.authorEdema, Richard
dc.contributor.authorMonjane, Ade´rito L
dc.contributor.authorRybicki, Edward P
dc.contributor.authorThomson, Jennifer A
dc.contributor.authorVasani, Arvind
dc.date.accessioned2016-07-21T13:11:36Z
dc.date.available2016-07-21T13:11:36Z
dc.date.issued2007
dc.date.updated2016-07-21T13:10:21Z
dc.description.abstractMaize streak virus (MSV) contributes significantly to the problem of extremely low African maize yields. Whilst a diverse range of MSV and MSV-like viruses are endemic in sub-Saharan Africa and neighbouring islands, only a single group of maize-adapted variants – MSV subtypes A1–A6 – causes severe enough disease in maize to influence yields substantially. In order to assist in designing effective strategies to control MSV in maize, a large survey covering 155 locations was conducted to assess the diversity, distribution and genetic characteristics of the Ugandan MSV-A population. PCR–restriction fragment-length polymorphism analyses of 391 virus isolates identified 49 genetic variants. Sixty-two full-genome sequences were determined, 52 of which were detectably recombinant. All but two recombinants contained predominantly MSV-A1-like sequences. Of the ten distinct recombination events observed, seven involved inter-MSV-A subtype recombination and three involved intra-MSV-A1 recombination. One of the intra-MSV-A1 recombinants, designated MSV-A1UgIII, accounted for .60 % of all MSV infections sampled throughout Uganda. Although recombination may be an important factor in the emergence of novel geminivirus variants, it is demonstrated that its characteristics in MSV are quite different from those observed in related African cassava-infecting geminivirus species.en_ZA
dc.identifierhttp://dx.doi.org/10.1099/vir.0.83144-0
dc.identifier.apacitationOwor, B. E., Martin, D. P., Shepherd, D. N., Edema, R., , Rybicki, E. P., ... Vasani, A. (2007). Genetic analysis of maize streak virus isolates from Uganda reveals widespread distribution of a recombinant variant. <i>Journal of General Virology</i>, http://hdl.handle.net/11427/20571en_ZA
dc.identifier.chicagocitationOwor, Betty E, Darren P Martin, Dionne N Shepherd, Richard Edema, , Edward P Rybicki, Jennifer A Thomson, and Arvind Vasani "Genetic analysis of maize streak virus isolates from Uganda reveals widespread distribution of a recombinant variant." <i>Journal of General Virology</i> (2007) http://hdl.handle.net/11427/20571en_ZA
dc.identifier.citationOwor, B. E., Martin, D. P., Shepherd, D. N., Edema, R., Monjane, A. L., Rybicki, E. P., ... & Varsani, A. (2007). Genetic analysis of maize streak virus isolates from Uganda reveals widespread distribution of a recombinant variant. Journal of general virology, 88(11), 3154-3165.en_ZA
dc.identifier.issn0022-1317en_ZA
dc.identifier.ris TY - Journal Article AU - Owor, Betty E AU - Martin, Darren P AU - Shepherd, Dionne N AU - Edema, Richard AU - Monjane, Ade´rito L AU - Rybicki, Edward P AU - Thomson, Jennifer A AU - Vasani, Arvind AB - Maize streak virus (MSV) contributes significantly to the problem of extremely low African maize yields. Whilst a diverse range of MSV and MSV-like viruses are endemic in sub-Saharan Africa and neighbouring islands, only a single group of maize-adapted variants – MSV subtypes A1–A6 – causes severe enough disease in maize to influence yields substantially. In order to assist in designing effective strategies to control MSV in maize, a large survey covering 155 locations was conducted to assess the diversity, distribution and genetic characteristics of the Ugandan MSV-A population. PCR–restriction fragment-length polymorphism analyses of 391 virus isolates identified 49 genetic variants. Sixty-two full-genome sequences were determined, 52 of which were detectably recombinant. All but two recombinants contained predominantly MSV-A1-like sequences. Of the ten distinct recombination events observed, seven involved inter-MSV-A subtype recombination and three involved intra-MSV-A1 recombination. One of the intra-MSV-A1 recombinants, designated MSV-A1UgIII, accounted for .60 % of all MSV infections sampled throughout Uganda. Although recombination may be an important factor in the emergence of novel geminivirus variants, it is demonstrated that its characteristics in MSV are quite different from those observed in related African cassava-infecting geminivirus species. DA - 2007 DB - OpenUCT DP - University of Cape Town J1 - Journal of General Virology LK - https://open.uct.ac.za PB - University of Cape Town PY - 2007 SM - 0022-1317 T1 - Genetic analysis of maize streak virus isolates from Uganda reveals widespread distribution of a recombinant variant TI - Genetic analysis of maize streak virus isolates from Uganda reveals widespread distribution of a recombinant variant UR - http://hdl.handle.net/11427/20571 ER - en_ZA
dc.identifier.urihttp://hdl.handle.net/11427/20571
dc.identifier.urihttp://jgv.microbiologyresearch.org/content/journal/jgv/10.1099/vir.0.83144-0#tab2
dc.identifier.vancouvercitationOwor BE, Martin DP, Shepherd DN, Edema R, , Rybicki EP, et al. Genetic analysis of maize streak virus isolates from Uganda reveals widespread distribution of a recombinant variant. Journal of General Virology. 2007; http://hdl.handle.net/11427/20571.en_ZA
dc.languageengen_ZA
dc.publisherMicrobiology Societyen_ZA
dc.publisher.institutionUniversity of Cape Town
dc.sourceJournal of General Virologyen_ZA
dc.source.urihttp://jgv.microbiologyresearch.org/content/journal/jgv
dc.subject.otherGenetic analysis
dc.subject.othermaize streak virus
dc.subject.otherUganda
dc.subject.otherrecombinant variant
dc.titleGenetic analysis of maize streak virus isolates from Uganda reveals widespread distribution of a recombinant variant.en_ZA
dc.typeJournal Articleen_ZA
uct.type.filetypeText
uct.type.filetypeImage
uct.type.publicationResearchen_ZA
uct.type.resourceArticleen_ZA
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