Turnip curly top virus, a highly divergent geminivirus infecting turnip in Iran

dc.contributor.authorBriddon, Rob W
dc.contributor.authorHeydarnejad, Jahangir
dc.contributor.authorKhosrowfar, Fakhrosadat
dc.contributor.authorMassumi, Hossain
dc.contributor.authorMartin, Darren P
dc.contributor.authorVarsani, Arvin
dc.date.accessioned2016-07-26T10:20:37Z
dc.date.available2016-07-26T10:20:37Z
dc.date.issued2010
dc.date.updated2016-07-26T10:19:23Z
dc.description.abstractFrom 2006 onwards turnip crops in Fars province, Iran, have been noted with unusual leaf curling and vein swelling symptoms which are characteristic of the leafhopper-transmitted viruses of the genus Curtovirus (family Geminiviridae). Rolling circle amplification was used to clone viruses from five turnip isolates exhibiting leaf curl symptoms. Analysis of the sequences showed them to have >93% sequence identity and to be distinct from all other geminiviruses previously characterised. Analysis of the sequence of this virus, for which we propose the name Turnip curly top virus (TCTV), showed it to have a genome arrangement in the complementary-sense similar to that of curtoviruses (consisting of four overlapping genes) but only two open reading frames in the virion-sense (the curtoviruses encode three). The complementarysense genes are homologous to those of curtoviruses but show little sequence identity to their curtovirus homologs, with the exception of the product of the C4 open reading frame (ORF) which shows ∼70.6% amino acid sequence identity to the C4 of the North American curtoviruses, Pepper curly top virus and Beet mild curly top virus. For curtoviruses the C4 protein is a symptom determinant, which likely explains the similarity of TCTV symptoms to those of curtoviruses. In the virion-sense the predicted product of the V2 ORF of TCTV shows no significant similarity with any proteins in the databases whereas the product of the V1 ORF (encoding the coat protein [CP] of geminiviruses) shows low levels of sequence identity to the CPs of curtoviruses. These findings show TCTV to be a highly divergent geminivirus with similarities to viruses of the genus curtovirus. The significance of these findings, particularly the taxonomic implications are discussed.en_ZA
dc.identifierhttp://dx.doi.org/10.1016/j.virusres.2010.05.016
dc.identifier.apacitationBriddon, R. W., Heydarnejad, J., Khosrowfar, F., Massumi, H., Martin, D. P., & Varsani, A. (2010). Turnip curly top virus, a highly divergent geminivirus infecting turnip in Iran. <i>Virus Research</i>, http://hdl.handle.net/11427/20753en_ZA
dc.identifier.chicagocitationBriddon, Rob W, Jahangir Heydarnejad, Fakhrosadat Khosrowfar, Hossain Massumi, Darren P Martin, and Arvin Varsani "Turnip curly top virus, a highly divergent geminivirus infecting turnip in Iran." <i>Virus Research</i> (2010) http://hdl.handle.net/11427/20753en_ZA
dc.identifier.citationBriddon, R. W., Heydarnejad, J., Khosrowfar, F., Massumi, H., Martin, D. P., & Varsani, A. (2010). Turnip curly top virus, a highly divergent geminivirus infecting turnip in Iran. Virus research, 152(1), 169-175.en_ZA
dc.identifier.issn0168-1702en_ZA
dc.identifier.ris TY - Journal Article AU - Briddon, Rob W AU - Heydarnejad, Jahangir AU - Khosrowfar, Fakhrosadat AU - Massumi, Hossain AU - Martin, Darren P AU - Varsani, Arvin AB - From 2006 onwards turnip crops in Fars province, Iran, have been noted with unusual leaf curling and vein swelling symptoms which are characteristic of the leafhopper-transmitted viruses of the genus Curtovirus (family Geminiviridae). Rolling circle amplification was used to clone viruses from five turnip isolates exhibiting leaf curl symptoms. Analysis of the sequences showed them to have >93% sequence identity and to be distinct from all other geminiviruses previously characterised. Analysis of the sequence of this virus, for which we propose the name Turnip curly top virus (TCTV), showed it to have a genome arrangement in the complementary-sense similar to that of curtoviruses (consisting of four overlapping genes) but only two open reading frames in the virion-sense (the curtoviruses encode three). The complementarysense genes are homologous to those of curtoviruses but show little sequence identity to their curtovirus homologs, with the exception of the product of the C4 open reading frame (ORF) which shows ∼70.6% amino acid sequence identity to the C4 of the North American curtoviruses, Pepper curly top virus and Beet mild curly top virus. For curtoviruses the C4 protein is a symptom determinant, which likely explains the similarity of TCTV symptoms to those of curtoviruses. In the virion-sense the predicted product of the V2 ORF of TCTV shows no significant similarity with any proteins in the databases whereas the product of the V1 ORF (encoding the coat protein [CP] of geminiviruses) shows low levels of sequence identity to the CPs of curtoviruses. These findings show TCTV to be a highly divergent geminivirus with similarities to viruses of the genus curtovirus. The significance of these findings, particularly the taxonomic implications are discussed. DA - 2010 DB - OpenUCT DP - University of Cape Town J1 - Virus Research LK - https://open.uct.ac.za PB - University of Cape Town PY - 2010 SM - 0168-1702 T1 - Turnip curly top virus, a highly divergent geminivirus infecting turnip in Iran TI - Turnip curly top virus, a highly divergent geminivirus infecting turnip in Iran UR - http://hdl.handle.net/11427/20753 ER - en_ZA
dc.identifier.urihttp://hdl.handle.net/11427/20753
dc.identifier.urihttp://www.sciencedirect.com/science/article/pii/S0168170210001838
dc.identifier.vancouvercitationBriddon RW, Heydarnejad J, Khosrowfar F, Massumi H, Martin DP, Varsani A. Turnip curly top virus, a highly divergent geminivirus infecting turnip in Iran. Virus Research. 2010; http://hdl.handle.net/11427/20753.en_ZA
dc.languageengen_ZA
dc.publisherElsevieren_ZA
dc.publisher.institutionUniversity of Cape Town
dc.sourceVirus Researchen_ZA
dc.source.urihttp://www.sciencedirect.com/science/article/pii/S0168170210001838
dc.subject.otherGeminivirus
dc.subject.otherCurtovirus
dc.subject.otherTurnip
dc.titleTurnip curly top virus, a highly divergent geminivirus infecting turnip in Iranen_ZA
dc.typeJournal Articleen_ZA
uct.type.filetypeText
uct.type.filetypeImage
uct.type.publicationResearchen_ZA
uct.type.resourceArticleen_ZA
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