Transkingdom Analysis of the Female Reproductive Tract Reveals Bacteriophages form Communities

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dc.contributor.authorMadere, Ferralita S
dc.contributor.authorSohn, Michael
dc.contributor.authorWinbush, Angelina K
dc.contributor.authorBarr, BreĆ³na
dc.contributor.authorGrier, Alex
dc.contributor.authorPalumbo, Cal
dc.contributor.authorJava, James
dc.contributor.authorMeiring, Tracy
dc.contributor.authorWilliamson, Anna-Lise
dc.contributor.authorBekker, Linda-Gail
dc.contributor.authorAdler, David H
dc.contributor.authorMonaco, Cynthia L
dc.date.accessioned2022-04-11T08:16:43Z
dc.date.available2022-04-11T08:16:43Z
dc.date.issued2022-02-19
dc.date.updated2022-02-24T14:50:28Z
dc.description.abstractThe female reproductive tract (FRT) microbiome plays a vital role in maintaining vaginal health. Viruses are key regulators of other microbial ecosystems, but little is known about how the FRT viruses (virome), particularly bacteriophages that comprise the phageome, impact FRT health and dysbiosis. We hypothesize that bacterial vaginosis (BV) is associated with altered FRT phageome diversity, transkingdom interplay, and bacteriophage discriminate taxa. Here, we conducted a retrospective, longitudinal analysis of vaginal swabs collected from 54 BV-positive and 46 BV-negative South African women. Bacteriome analysis revealed samples clustered into five distinct bacterial community groups (CGs), and further, bacterial alpha diversity was significantly associated with BV. Virome analysis on a subset of baseline samples showed FRT bacteriophages clustering into novel viral state types (VSTs), a viral community clustering system based on virome composition and abundance. Distinct BV bacteriophage signatures included increased alpha diversity along with discriminant <i>Bacillus</i>, <i>Burkholderia,</i> and <i>Escherichia</i> bacteriophages. Bacteriophage-bacteria transkingdom associations were also identified between <i>Bacillus</i> and <i>Burkholderia</i> viruses and BV-associated bacteria, providing key insights for future studies elucidating the transkingdom interactions driving BV-associated microbiome perturbations. In this cohort, bacteriophage-bacterial associations suggest complex interactions, which may play a role in the establishment and maintenance of BV.en_US
dc.identifierdoi: 10.3390/v14020430
dc.identifier.apacitationMadere, F. S., Sohn, M., Winbush, A. K., Barr, B., Grier, A., Palumbo, C., ... Monaco, C. L. (2022). Transkingdom Analysis of the Female Reproductive Tract Reveals Bacteriophages form Communities. <i>Viruses</i>, 14(2), 430. http://hdl.handle.net/11427/36324en_ZA
dc.identifier.chicagocitationMadere, Ferralita S, Michael Sohn, Angelina K Winbush, BreĆ³na Barr, Alex Grier, Cal Palumbo, James Java, et al "Transkingdom Analysis of the Female Reproductive Tract Reveals Bacteriophages form Communities." <i>Viruses</i> 14, 2. (2022): 430. http://hdl.handle.net/11427/36324en_ZA
dc.identifier.citationMadere, F.S., Sohn, M., Winbush, A.K., Barr, B., Grier, A., Palumbo, C., Java, J. & Meiring, T. et al. 2022. Transkingdom Analysis of the Female Reproductive Tract Reveals Bacteriophages form Communities. <i>Viruses.</i> 14(2):430. http://hdl.handle.net/11427/36324en_ZA
dc.identifier.ris TY - Journal Article AU - Madere, Ferralita S AU - Sohn, Michael AU - Winbush, Angelina K AU - Barr, BreĆ³na AU - Grier, Alex AU - Palumbo, Cal AU - Java, James AU - Meiring, Tracy AU - Williamson, Anna-Lise AU - Bekker, Linda-Gail AU - Adler, David H AU - Monaco, Cynthia L AB - The female reproductive tract (FRT) microbiome plays a vital role in maintaining vaginal health. Viruses are key regulators of other microbial ecosystems, but little is known about how the FRT viruses (virome), particularly bacteriophages that comprise the phageome, impact FRT health and dysbiosis. We hypothesize that bacterial vaginosis (BV) is associated with altered FRT phageome diversity, transkingdom interplay, and bacteriophage discriminate taxa. Here, we conducted a retrospective, longitudinal analysis of vaginal swabs collected from 54 BV-positive and 46 BV-negative South African women. Bacteriome analysis revealed samples clustered into five distinct bacterial community groups (CGs), and further, bacterial alpha diversity was significantly associated with BV. Virome analysis on a subset of baseline samples showed FRT bacteriophages clustering into novel viral state types (VSTs), a viral community clustering system based on virome composition and abundance. Distinct BV bacteriophage signatures included increased alpha diversity along with discriminant <i>Bacillus</i>, <i>Burkholderia,</i> and <i>Escherichia</i> bacteriophages. Bacteriophage-bacteria transkingdom associations were also identified between <i>Bacillus</i> and <i>Burkholderia</i> viruses and BV-associated bacteria, providing key insights for future studies elucidating the transkingdom interactions driving BV-associated microbiome perturbations. In this cohort, bacteriophage-bacterial associations suggest complex interactions, which may play a role in the establishment and maintenance of BV. DA - 2022-02-19 DB - OpenUCT DP - University of Cape Town IS - 2 J1 - Viruses KW - virome KW - microbiome KW - bacterial vaginosis KW - bacteriophage KW - transkingdom associations KW - female reproductive tract KW - HIV LK - https://open.uct.ac.za PY - 2022 T1 - Transkingdom Analysis of the Female Reproductive Tract Reveals Bacteriophages form Communities TI - Transkingdom Analysis of the Female Reproductive Tract Reveals Bacteriophages form Communities UR - http://hdl.handle.net/11427/36324 ER - en_ZA
dc.identifier.urihttp://hdl.handle.net/11427/36324
dc.identifier.vancouvercitationMadere FS, Sohn M, Winbush AK, Barr B, Grier A, Palumbo C, et al. Transkingdom Analysis of the Female Reproductive Tract Reveals Bacteriophages form Communities. Viruses. 2022;14(2):430. http://hdl.handle.net/11427/36324.en_ZA
dc.language.isoenen_US
dc.publisher.departmentInstitute of Infectious Disease and Molecular Medicineen_US
dc.publisher.facultyFaculty of Health Sciencesen_US
dc.rights.urihttp://creativecommons.org/licenses/by/4.0/en_US
dc.sourceVirusesen_US
dc.source.journalissue2en_US
dc.source.journalvolume14en_US
dc.source.pagination430en_US
dc.subjectviromeen_US
dc.subjectmicrobiome
dc.subjectbacterial vaginosis
dc.subjectbacteriophage
dc.subjecttranskingdom associations
dc.subjectfemale reproductive tract
dc.subjectHIV
dc.titleTranskingdom Analysis of the Female Reproductive Tract Reveals Bacteriophages form Communitiesen_US
dc.typeJournal Articleen_US
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