Microbial oxidation of dodecane and tridecane into a,w-dicarboxylic acids using recombinant Yarrowia lipolytica

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dc.contributor.advisorHarrison, STLen_ZA
dc.contributor.advisorSmit, Martieen_ZA
dc.contributor.advisorRawatlal, Randhiren_ZA
dc.contributor.authorDiedericks, Danieen_ZA
dc.date.accessioned2014-07-31T11:13:29Z
dc.date.available2014-07-31T11:13:29Z
dc.date.issued2007en_ZA
dc.descriptionIncludes bibliographical references (leaves 137-150).
dc.description.abstracta,w-Dicarboxylic acids are reactive intermediates, widely used as raw materials to synthesise products such as perfumes, hot-melting adhesives, engineering plastics and high quality lubricants. These acids can be obtained via chemical or biological routes by using various feedstocks such as linear alkanes. Linear alkanes are chemically inert; hence, the production of reactive products requires complex and sophisticated reactions catalysed by either catalysts or enzymes. However, simultaneous by-product formation on chemical synthesis increases production cost and limits commercial availability, preventing their widespread application. Biological routes alternatively, selectively transform linear alkanes into fatty and a,w-dicarboxylic acids. Linear alkanes, due to their relative abundance and increased availability, following the expansion of gas-to-liquid fuels technology, are viewed as prospective feedstocks for the microbial production of a,w-dicarboxylic acids. The commercialisation of the biological conversion of linear alkanes is constrained by the low turnover frequency of the cytochrome P450 hydroxylase complex responsible for catalysing the first and rate limiting step of the monoterminal and diterminal pathways. Low product yields may be caused by the further catabolism of a,w-dicarboxylic acids, through the ~-oxidation pathway into energy, carbon dioxide and water. To prevent this, metabolic engineering techniques can be applied to prevent ~-oxidation by disrupting the genes encoding the enzyme catalysing the first step in the~-oxidation pathway. The specific productivity of bioconversion can then be increased further by over-expressing the genes encoding the cytochrome P450 hydroxylase complex. Recombinant Yarrowia lipolytica strains TVN 497, TVN 499, TVN 501 and TVN 502 were developed in such a manner by the collaborating research group at the University of the Free State and made available for this research.en_ZA
dc.identifier.apacitationDiedericks, D. (2007). <i>Microbial oxidation of dodecane and tridecane into a,w-dicarboxylic acids using recombinant Yarrowia lipolytica</i>. (Thesis). University of Cape Town ,Faculty of Engineering & the Built Environment ,Department of Chemical Engineering. Retrieved from http://hdl.handle.net/11427/5377en_ZA
dc.identifier.chicagocitationDiedericks, Danie. <i>"Microbial oxidation of dodecane and tridecane into a,w-dicarboxylic acids using recombinant Yarrowia lipolytica."</i> Thesis., University of Cape Town ,Faculty of Engineering & the Built Environment ,Department of Chemical Engineering, 2007. http://hdl.handle.net/11427/5377en_ZA
dc.identifier.citationDiedericks, D. 2007. Microbial oxidation of dodecane and tridecane into a,w-dicarboxylic acids using recombinant Yarrowia lipolytica. University of Cape Town.en_ZA
dc.identifier.risTY - Thesis / Dissertation AU - Diedericks, Danie AB - a,w-Dicarboxylic acids are reactive intermediates, widely used as raw materials to synthesise products such as perfumes, hot-melting adhesives, engineering plastics and high quality lubricants. These acids can be obtained via chemical or biological routes by using various feedstocks such as linear alkanes. Linear alkanes are chemically inert; hence, the production of reactive products requires complex and sophisticated reactions catalysed by either catalysts or enzymes. However, simultaneous by-product formation on chemical synthesis increases production cost and limits commercial availability, preventing their widespread application. Biological routes alternatively, selectively transform linear alkanes into fatty and a,w-dicarboxylic acids. Linear alkanes, due to their relative abundance and increased availability, following the expansion of gas-to-liquid fuels technology, are viewed as prospective feedstocks for the microbial production of a,w-dicarboxylic acids. The commercialisation of the biological conversion of linear alkanes is constrained by the low turnover frequency of the cytochrome P450 hydroxylase complex responsible for catalysing the first and rate limiting step of the monoterminal and diterminal pathways. Low product yields may be caused by the further catabolism of a,w-dicarboxylic acids, through the ~-oxidation pathway into energy, carbon dioxide and water. To prevent this, metabolic engineering techniques can be applied to prevent ~-oxidation by disrupting the genes encoding the enzyme catalysing the first step in the~-oxidation pathway. The specific productivity of bioconversion can then be increased further by over-expressing the genes encoding the cytochrome P450 hydroxylase complex. Recombinant Yarrowia lipolytica strains TVN 497, TVN 499, TVN 501 and TVN 502 were developed in such a manner by the collaborating research group at the University of the Free State and made available for this research. DA - 2007 DB - OpenUCT DP - University of Cape Town LK - https://open.uct.ac.za PB - University of Cape Town PY - 2007 T1 - Microbial oxidation of dodecane and tridecane into a,w-dicarboxylic acids using recombinant Yarrowia lipolytica TI - Microbial oxidation of dodecane and tridecane into a,w-dicarboxylic acids using recombinant Yarrowia lipolytica UR - http://hdl.handle.net/11427/5377 ER -en_ZA
dc.identifier.urihttp://hdl.handle.net/11427/5377
dc.identifier.vancouvercitationDiedericks D. Microbial oxidation of dodecane and tridecane into a,w-dicarboxylic acids using recombinant Yarrowia lipolytica. [Thesis]. University of Cape Town ,Faculty of Engineering & the Built Environment ,Department of Chemical Engineering, 2007 [cited yyyy month dd]. Available from: http://hdl.handle.net/11427/5377en_ZA
dc.language.isoengen_ZA
dc.publisher.departmentCentre for Bioprocess Engineering Researchen_ZA
dc.publisher.facultyFaculty of Engineering and the Built Environment
dc.publisher.institutionUniversity of Cape Town
dc.subject.otherBioprocess Engineeringen_ZA
dc.titleMicrobial oxidation of dodecane and tridecane into a,w-dicarboxylic acids using recombinant Yarrowia lipolyticaen_ZA
dc.typeMaster Thesis
dc.type.qualificationlevelMasters
dc.type.qualificationnameMScen_ZA
uct.type.filetypeText
uct.type.filetypeImage
uct.type.publicationResearchen_ZA
uct.type.resourceThesisen_ZA
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