Investigation of the role of dietary myo-inositol hexakisphosphate (phytate) on the relative risk of calcium oxalate kidney stone formation in black and white male South African subjects

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dc.contributor.advisorRodgers, Allenen_ZA
dc.contributor.advisorRavenscroft, Neilen_ZA
dc.contributor.authorLesotho, Ntlamaen_ZA
dc.date.accessioned2014-11-05T17:41:56Z
dc.date.available2014-11-05T17:41:56Z
dc.date.issued2005en_ZA
dc.descriptionIncludes bibliographical references.en_ZA
dc.description.abstractPrevious studies have shown that caJclum oxalate (CaOx) stone-formers have lower urinary concentrations of myo-inositol hexakisphosphate (phytate or IPe) than healthy individuals, that dietary intake of this substance leads to its increased urinary excretion and that it is an inhibitor of CaOx nucleation and growth In South Africa it has been reported that the black population has a higher dietary phytate intake than whites. The present study was undertaken to test the hypothesis that South African black subjects have higher urinary phytate levels than their white cOLlflterparts and that this contributes to the relative rarity of caOx kidney stone disease in this population group A modified indirect extraction/photometry method to measure urinary IPe was designed, developed and tested in the present study. This assay was then used to measure IPo in the urine of rural black and urban white subjects while on their free unrestricted diets In addition, urban black and white subjects each followed IPo-restricted followed by lPG-rich dietary protocols for a period of three days Urines were collected after administration of each protocol and were again analysed for IPe using the newly developed assay. Urines were then used in several crystallization experiments to measure the CaOx metastable limit, "C-oxalate deposition kinetics and inhiOition of CaOx crystal aggregation. The results showed that while on their free diets, rural blacks excreted significantly less IPs than urban whites despite their previously reported higher dietary intake of this substance This suggests that the renal handling of dietary IPen_ZA
dc.identifier.apacitationLesotho, N. (2005). <i>Investigation of the role of dietary myo-inositol hexakisphosphate (phytate) on the relative risk of calcium oxalate kidney stone formation in black and white male South African subjects</i>. (Thesis). University of Cape Town ,Faculty of Science ,Department of Chemistry. Retrieved from http://hdl.handle.net/11427/9279en_ZA
dc.identifier.chicagocitationLesotho, Ntlama. <i>"Investigation of the role of dietary myo-inositol hexakisphosphate (phytate) on the relative risk of calcium oxalate kidney stone formation in black and white male South African subjects."</i> Thesis., University of Cape Town ,Faculty of Science ,Department of Chemistry, 2005. http://hdl.handle.net/11427/9279en_ZA
dc.identifier.citationLesotho, N. 2005. Investigation of the role of dietary myo-inositol hexakisphosphate (phytate) on the relative risk of calcium oxalate kidney stone formation in black and white male South African subjects. University of Cape Town.en_ZA
dc.identifier.ris TY - Thesis / Dissertation AU - Lesotho, Ntlama AB - Previous studies have shown that caJclum oxalate (CaOx) stone-formers have lower urinary concentrations of myo-inositol hexakisphosphate (phytate or IPe) than healthy individuals, that dietary intake of this substance leads to its increased urinary excretion and that it is an inhibitor of CaOx nucleation and growth In South Africa it has been reported that the black population has a higher dietary phytate intake than whites. The present study was undertaken to test the hypothesis that South African black subjects have higher urinary phytate levels than their white cOLlflterparts and that this contributes to the relative rarity of caOx kidney stone disease in this population group A modified indirect extraction/photometry method to measure urinary IPe was designed, developed and tested in the present study. This assay was then used to measure IPo in the urine of rural black and urban white subjects while on their free unrestricted diets In addition, urban black and white subjects each followed IPo-restricted followed by lPG-rich dietary protocols for a period of three days Urines were collected after administration of each protocol and were again analysed for IPe using the newly developed assay. Urines were then used in several crystallization experiments to measure the CaOx metastable limit, "C-oxalate deposition kinetics and inhiOition of CaOx crystal aggregation. The results showed that while on their free diets, rural blacks excreted significantly less IPs than urban whites despite their previously reported higher dietary intake of this substance This suggests that the renal handling of dietary IP DA - 2005 DB - OpenUCT DP - University of Cape Town LK - https://open.uct.ac.za PB - University of Cape Town PY - 2005 T1 - Investigation of the role of dietary myo-inositol hexakisphosphate (phytate) on the relative risk of calcium oxalate kidney stone formation in black and white male South African subjects TI - Investigation of the role of dietary myo-inositol hexakisphosphate (phytate) on the relative risk of calcium oxalate kidney stone formation in black and white male South African subjects UR - http://hdl.handle.net/11427/9279 ER - en_ZA
dc.identifier.urihttp://hdl.handle.net/11427/9279
dc.identifier.vancouvercitationLesotho N. Investigation of the role of dietary myo-inositol hexakisphosphate (phytate) on the relative risk of calcium oxalate kidney stone formation in black and white male South African subjects. [Thesis]. University of Cape Town ,Faculty of Science ,Department of Chemistry, 2005 [cited yyyy month dd]. Available from: http://hdl.handle.net/11427/9279en_ZA
dc.language.isoengen_ZA
dc.publisher.departmentDepartment of Chemistryen_ZA
dc.publisher.facultyFaculty of Scienceen_ZA
dc.publisher.institutionUniversity of Cape Town
dc.subject.otherChemistryen_ZA
dc.titleInvestigation of the role of dietary myo-inositol hexakisphosphate (phytate) on the relative risk of calcium oxalate kidney stone formation in black and white male South African subjectsen_ZA
dc.typeMaster Thesis
dc.type.qualificationlevelMasters
dc.type.qualificationnameMScen_ZA
uct.type.filetypeText
uct.type.filetypeImage
uct.type.publicationResearchen_ZA
uct.type.resourceThesisen_ZA
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