Characterisation of innate fungal recognition in the lung

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dc.contributor.authorFaro-Trindade, Inêsen_ZA
dc.contributor.authorWillment, Janet Aen_ZA
dc.contributor.authorKerrigan, Ann Men_ZA
dc.contributor.authorRedelinghuys, Pierreen_ZA
dc.contributor.authorHadebe, Sabeloen_ZA
dc.contributor.authorReid, Delyth Men_ZA
dc.contributor.authorSrinivasan, Narenen_ZA
dc.contributor.authorWainwright, Helenen_ZA
dc.contributor.authorLang, Dirk Men_ZA
dc.contributor.authorSteele, Chaden_ZA
dc.date.accessioned2015-11-23T12:35:20Z
dc.date.available2015-11-23T12:35:20Z
dc.date.issued2012en_ZA
dc.description.abstractThe innate recognition of fungi by leukocytes is mediated by pattern recognition receptors (PRR), such as Dectin-1, and is thought to occur at the cell surface triggering intracellular signalling cascades which lead to the induction of protective host responses. In the lung, this recognition is aided by surfactant which also serves to maintain the balance between inflammation and pulmonary function, although the underlying mechanisms are unknown. Here we have explored pulmonary innate recognition of a variety of fungal particles, including zymosan, Candida albicans and Aspergillus fumigatus , and demonstrate that opsonisation with surfactant components can limit inflammation by reducing host-cell fungal interactions. However, we found that this opsonisation does not contribute directly to innate fungal recognition and that this process is mediated through non-opsonic PRRs, including Dectin-1. Moreover, we found that pulmonary inflammatory responses to resting Aspergillus conidia were initiated by these PRRs in acidified phagolysosomes, following the uptake of fungal particles by leukocytes. Our data therefore provides crucial new insights into the mechanisms by which surfactant can maintain pulmonary function in the face of microbial challenge, and defines the phagolysosome as a novel intracellular compartment involved in the innate sensing of extracellular pathogens in the lung.en_ZA
dc.identifier.apacitationFaro-Trindade, I., Willment, J. A., Kerrigan, A. M., Redelinghuys, P., Hadebe, S., Reid, D. M., ... Steele, C. (2012). Characterisation of innate fungal recognition in the lung. <i>PLoS One</i>, http://hdl.handle.net/11427/15324en_ZA
dc.identifier.chicagocitationFaro-Trindade, Inês, Janet A Willment, Ann M Kerrigan, Pierre Redelinghuys, Sabelo Hadebe, Delyth M Reid, Naren Srinivasan, Helen Wainwright, Dirk M Lang, and Chad Steele "Characterisation of innate fungal recognition in the lung." <i>PLoS One</i> (2012) http://hdl.handle.net/11427/15324en_ZA
dc.identifier.citationFaro-Trindade, I., Willment, J. A., Kerrigan, A. M., Redelinghuys, P., Hadebe, S., Reid, D. M., ... & Brown, G. D. (2012). Characterisation of innate fungal recognition in the lung. PloS one, 7(4), e35675. doi:10.1371/journal.pone.0035675en_ZA
dc.identifier.ris TY - Journal Article AU - Faro-Trindade, Inês AU - Willment, Janet A AU - Kerrigan, Ann M AU - Redelinghuys, Pierre AU - Hadebe, Sabelo AU - Reid, Delyth M AU - Srinivasan, Naren AU - Wainwright, Helen AU - Lang, Dirk M AU - Steele, Chad AB - The innate recognition of fungi by leukocytes is mediated by pattern recognition receptors (PRR), such as Dectin-1, and is thought to occur at the cell surface triggering intracellular signalling cascades which lead to the induction of protective host responses. In the lung, this recognition is aided by surfactant which also serves to maintain the balance between inflammation and pulmonary function, although the underlying mechanisms are unknown. Here we have explored pulmonary innate recognition of a variety of fungal particles, including zymosan, Candida albicans and Aspergillus fumigatus , and demonstrate that opsonisation with surfactant components can limit inflammation by reducing host-cell fungal interactions. However, we found that this opsonisation does not contribute directly to innate fungal recognition and that this process is mediated through non-opsonic PRRs, including Dectin-1. Moreover, we found that pulmonary inflammatory responses to resting Aspergillus conidia were initiated by these PRRs in acidified phagolysosomes, following the uptake of fungal particles by leukocytes. Our data therefore provides crucial new insights into the mechanisms by which surfactant can maintain pulmonary function in the face of microbial challenge, and defines the phagolysosome as a novel intracellular compartment involved in the innate sensing of extracellular pathogens in the lung. DA - 2012 DB - OpenUCT DO - 10.1371/journal.pone.0035675 DP - University of Cape Town J1 - PLoS One LK - https://open.uct.ac.za PB - University of Cape Town PY - 2012 T1 - Characterisation of innate fungal recognition in the lung TI - Characterisation of innate fungal recognition in the lung UR - http://hdl.handle.net/11427/15324 ER - en_ZA
dc.identifier.urihttp://hdl.handle.net/11427/15324
dc.identifier.urihttp://dx.doi.org/10.1371/journal.pone.0035675
dc.identifier.vancouvercitationFaro-Trindade I, Willment JA, Kerrigan AM, Redelinghuys P, Hadebe S, Reid DM, et al. Characterisation of innate fungal recognition in the lung. PLoS One. 2012; http://hdl.handle.net/11427/15324.en_ZA
dc.language.isoengen_ZA
dc.publisherPublic Library of Scienceen_ZA
dc.publisher.departmentDivision of Immunologyen_ZA
dc.publisher.facultyFaculty of Health Sciencesen_ZA
dc.publisher.institutionUniversity of Cape Town
dc.rightsThis is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.en_ZA
dc.rights.holder© 2012 Faro-Trindade et alen_ZA
dc.rights.urihttp://creativecommons.org/licenses/by/4.0en_ZA
dc.sourcePLoS Oneen_ZA
dc.source.urihttp://journals.plos.org/plosoneen_ZA
dc.subject.otherSurfactantsen_ZA
dc.subject.otherInflammationen_ZA
dc.subject.otherAspergillusen_ZA
dc.subject.otherPhagosomesen_ZA
dc.subject.otherAspergillus fumigatusen_ZA
dc.subject.otherAlveolar macrophagesen_ZA
dc.subject.otherMacrophagesen_ZA
dc.subject.otherOpsonizationen_ZA
dc.titleCharacterisation of innate fungal recognition in the lungen_ZA
dc.typeJournal Articleen_ZA
uct.type.filetypeText
uct.type.filetypeImage
uct.type.publicationResearchen_ZA
uct.type.resourceArticleen_ZA
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