Design of an optimised fed-batch process for insulin precursor production in Pichia pastoris

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dc.contributor.advisorHarrison, STLen_ZA
dc.contributor.advisorMinnaar, Saneten_ZA
dc.contributor.authorBhardwaj, Vinayaken_ZA
dc.date.accessioned2014-12-26T14:15:27Z
dc.date.available2014-12-26T14:15:27Z
dc.date.issued2011en_ZA
dc.descriptionIncludes bibliographical references (leaves 98-103).en_ZA
dc.description.abstractThe increasing prevalence of diabetes worldwide has greatly increased the demand for insulin, a key type of treatment for many diabetics. For this purpose, the methylotrophic yeast Pichia pastoris has emerged as an additional microbial host for recombinant insulin production. A genetically modified Pichia pastoris MutS strain, engineered to produce the insulin precursor, was used as the experimental system in this study in order to optimise the insulin production process. The experimental system developed in this study employed a two-stage fed-batch feeding strategy in which growth was optimised by feeding glycerol to boost biomass followed by induction of the gene encoding insulin precursor by feeding methanol.en_ZA
dc.identifier.apacitationBhardwaj, V. (2011). <i>Design of an optimised fed-batch process for insulin precursor production in Pichia pastoris</i>. (Thesis). University of Cape Town ,Faculty of Engineering & the Built Environment ,Department of Chemical Engineering. Retrieved from http://hdl.handle.net/11427/10120en_ZA
dc.identifier.chicagocitationBhardwaj, Vinayak. <i>"Design of an optimised fed-batch process for insulin precursor production in Pichia pastoris."</i> Thesis., University of Cape Town ,Faculty of Engineering & the Built Environment ,Department of Chemical Engineering, 2011. http://hdl.handle.net/11427/10120en_ZA
dc.identifier.citationBhardwaj, V. 2011. Design of an optimised fed-batch process for insulin precursor production in Pichia pastoris. University of Cape Town.en_ZA
dc.identifier.risTY - Thesis / Dissertation AU - Bhardwaj, Vinayak AB - The increasing prevalence of diabetes worldwide has greatly increased the demand for insulin, a key type of treatment for many diabetics. For this purpose, the methylotrophic yeast Pichia pastoris has emerged as an additional microbial host for recombinant insulin production. A genetically modified Pichia pastoris MutS strain, engineered to produce the insulin precursor, was used as the experimental system in this study in order to optimise the insulin production process. The experimental system developed in this study employed a two-stage fed-batch feeding strategy in which growth was optimised by feeding glycerol to boost biomass followed by induction of the gene encoding insulin precursor by feeding methanol. DA - 2011 DB - OpenUCT DP - University of Cape Town LK - https://open.uct.ac.za PB - University of Cape Town PY - 2011 T1 - Design of an optimised fed-batch process for insulin precursor production in Pichia pastoris TI - Design of an optimised fed-batch process for insulin precursor production in Pichia pastoris UR - http://hdl.handle.net/11427/10120 ER -en_ZA
dc.identifier.urihttp://hdl.handle.net/11427/10120
dc.identifier.vancouvercitationBhardwaj V. Design of an optimised fed-batch process for insulin precursor production in Pichia pastoris. [Thesis]. University of Cape Town ,Faculty of Engineering & the Built Environment ,Department of Chemical Engineering, 2011 [cited yyyy month dd]. Available from: http://hdl.handle.net/11427/10120en_ZA
dc.language.isoengen_ZA
dc.publisher.departmentCentre for Bioprocess Engineering Researchen_ZA
dc.publisher.facultyFaculty of Engineering and the Built Environment
dc.publisher.institutionUniversity of Cape Town
dc.subject.otherBioprocess Engineeringen_ZA
dc.titleDesign of an optimised fed-batch process for insulin precursor production in Pichia pastorisen_ZA
dc.typeMaster Thesis
dc.type.qualificationlevelMasters
dc.type.qualificationnameMScen_ZA
uct.type.filetypeText
uct.type.filetypeImage
uct.type.publicationResearchen_ZA
uct.type.resourceThesisen_ZA
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