Importance of Candida infection and fluconazole resistance in women with vaginal discharge syndrome in Namibia

dc.contributor.authorDunaiski, Cara M.
dc.contributor.authorKock, Marleen M.
dc.contributor.authorJung, Hyunsul
dc.contributor.authorPeters, Remco P. H.
dc.date.accessioned2023-09-18T10:02:56Z
dc.date.available2023-09-18T10:02:56Z
dc.date.issued2022-08-15
dc.date.updated2022-08-21T03:10:30Z
dc.description.abstractAbstract Background Vaginal discharge syndrome (VDS) is a common condition. Clinical management targets sexually transmitted infections (STIs) and bacterial vaginosis (BV); there is limited focus on Candida infection as cause of VDS. Lack of Candida treatment coverage and, if present, antifungal resistance may result in VDS treatment failure. This study aimed to determine the prevalence of Candida infection, antifungal resistance, and coinfections in Namibian women with VDS. Methods A cross-sectional study was performed using 253 vaginal swabs from women with VDS in Namibia. Demographic data was collected, and phenotypic and molecular detection of Candida species was performed followed by fluconazole susceptibility testing of Candida isolates. BV was diagnosed using Nugent score microscopy; molecular detection of Chlamydia trachomatis, Neisseria gonorrhoeae and Trichomonas vaginalis was performed. Results Candida species was detected in 110/253 women (43%). Ninety women (36%) had Candida albicans and 24 (9.5%) had non-albicans Candida species. The non-albicans species detected were 19 (17%) Candida glabrata, 4.0 (3.5%) Candida krusei, and 1.0 (0.9%) Candida parapsilosis. Candida albicans were more frequently isolated in younger (p = 0.004) and pregnant women (p = 0.04) compared to non-albicans Candida species. Almost all (98%) Candida albicans isolates were susceptible to fluconazole while all non-albicans Candida species were fluconazole resistant. STIs were diagnosed in 92 women (36%): 30 (12%) with C. trachomatis, 11 (4.3%) N. gonorrhoeae, and 70 (28%) T. vaginalis; 98 (39%) women had BV. Candida infection alone was diagnosed in 30 women (12%), combined with STIs in 42 women (17%) and was concurrent with BV in 38 women (15%). Candida infection was more often detected in swabs from women without C. trachomatis detected (6.4% vs. 16%; OR 0.30; 95% CI 0.10–0.77, p = 0.006). Conclusions The high prevalence of Candida infection, especially those due to non-albicans Candida species that are resistant to fluconazole, is a great concern in our setting and may lead to poor treatment outcomes. Access to microbiological testing for Candida species in the context of syndromic management is warranted.
dc.identifier.apacitationDunaiski, Cara M., Kock, Marleen M., Jung, H., & Peters, Remco P. H. (2022). Importance of Candida infection and fluconazole resistance in women with vaginal discharge syndrome in Namibia. http://hdl.handle.net/11427/38719en_ZA
dc.identifier.chicagocitationDunaiski, Cara M., Marleen M. Kock, Hyunsul Jung, and Remco P. H. Peters "Importance of Candida infection and fluconazole resistance in women with vaginal discharge syndrome in Namibia." (2022) http://hdl.handle.net/11427/38719en_ZA
dc.identifier.citationAntimicrobial Resistance & Infection Control. 2022 Aug 15;11(1):104
dc.identifier.ris TY - Journal Article AU - Dunaiski, Cara M. AU - Kock, Marleen M. AU - Jung, Hyunsul AU - Peters, Remco P. H. AB - Abstract Background Vaginal discharge syndrome (VDS) is a common condition. Clinical management targets sexually transmitted infections (STIs) and bacterial vaginosis (BV); there is limited focus on Candida infection as cause of VDS. Lack of Candida treatment coverage and, if present, antifungal resistance may result in VDS treatment failure. This study aimed to determine the prevalence of Candida infection, antifungal resistance, and coinfections in Namibian women with VDS. Methods A cross-sectional study was performed using 253 vaginal swabs from women with VDS in Namibia. Demographic data was collected, and phenotypic and molecular detection of Candida species was performed followed by fluconazole susceptibility testing of Candida isolates. BV was diagnosed using Nugent score microscopy; molecular detection of Chlamydia trachomatis, Neisseria gonorrhoeae and Trichomonas vaginalis was performed. Results Candida species was detected in 110/253 women (43%). Ninety women (36%) had Candida albicans and 24 (9.5%) had non-albicans Candida species. The non-albicans species detected were 19 (17%) Candida glabrata, 4.0 (3.5%) Candida krusei, and 1.0 (0.9%) Candida parapsilosis. Candida albicans were more frequently isolated in younger (p = 0.004) and pregnant women (p = 0.04) compared to non-albicans Candida species. Almost all (98%) Candida albicans isolates were susceptible to fluconazole while all non-albicans Candida species were fluconazole resistant. STIs were diagnosed in 92 women (36%): 30 (12%) with C. trachomatis, 11 (4.3%) N. gonorrhoeae, and 70 (28%) T. vaginalis; 98 (39%) women had BV. Candida infection alone was diagnosed in 30 women (12%), combined with STIs in 42 women (17%) and was concurrent with BV in 38 women (15%). Candida infection was more often detected in swabs from women without C. trachomatis detected (6.4% vs. 16%; OR 0.30; 95% CI 0.10–0.77, p = 0.006). Conclusions The high prevalence of Candida infection, especially those due to non-albicans Candida species that are resistant to fluconazole, is a great concern in our setting and may lead to poor treatment outcomes. Access to microbiological testing for Candida species in the context of syndromic management is warranted. DA - 2022-08-15 DB - OpenUCT DP - University of Cape Town KW - Candida albicans KW - Non-albicans Candida species KW - Vaginal discharge syndrome KW - Antifungal susceptibility testing KW - Sexually transmitted infections KW - Namibia KW - Sub-Saharan Africa LK - https://open.uct.ac.za PY - 2022 T1 - Importance of Candida infection and fluconazole resistance in women with vaginal discharge syndrome in Namibia TI - Importance of Candida infection and fluconazole resistance in women with vaginal discharge syndrome in Namibia UR - http://hdl.handle.net/11427/38719 ER - en_ZA
dc.identifier.urihttps://doi.org/10.1186/s13756-022-01143-6
dc.identifier.urihttp://hdl.handle.net/11427/38719
dc.identifier.vancouvercitationDunaiski Cara M, Kock Marleen M, Jung H, Peters Remco P H. Importance of Candida infection and fluconazole resistance in women with vaginal discharge syndrome in Namibia. 2022; http://hdl.handle.net/11427/38719.en_ZA
dc.language.rfc3066en
dc.publisherBioMed Central
dc.rights.holderThe Author(s)
dc.subjectCandida albicans
dc.subjectNon-albicans Candida species
dc.subjectVaginal discharge syndrome
dc.subjectAntifungal susceptibility testing
dc.subjectSexually transmitted infections
dc.subjectNamibia
dc.subjectSub-Saharan Africa
dc.titleImportance of Candida infection and fluconazole resistance in women with vaginal discharge syndrome in Namibia
dc.typeJournal Article
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