Distribution of high-risk human papillomavirus genotypes among HIV-negative women with and without cervical intraepithelial neoplasia in South Africa

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dc.contributor.authorMcDonald, Alicia Cen_ZA
dc.contributor.authorDenny, Lynetteen_ZA
dc.contributor.authorWang, Chunhuien_ZA
dc.contributor.authorTsai, Wei-Yannen_ZA
dc.contributor.authorJr, Thomas C Wrighten_ZA
dc.contributor.authorKuhn, Louiseen_ZA
dc.date.accessioned2015-11-23T12:30:41Z
dc.date.available2015-11-23T12:30:41Z
dc.date.issued2012en_ZA
dc.description.abstractObjective Large studies describing the profile of high-risk Human papillomavirus (hrHPV) genotypes among women in sub-Saharan Africa are lacking. Here we describe the prevalence and distribution of hrHPV genotypes among HIV-negative women in South Africa, with and without cervical intraepithelial neoplasia (CIN). METHODS: We report data on 8,050 HIV-negative women, aged 17-65 years, recruited into three sequential studies undertaken in Cape Town, South Africa. Women had no history of previous cervical cancer screening. Cervical samples were tested for hrHPV DNA using the Hybrid Capture 2 (HC2) assay and all positive samples were genotyped using a PCR-based assay (Line Blot). Women underwent colposcopy and biopsy/endocervical curettage to determine CIN status. The prevalence and distribution of specific hrHPV genotypes were examined by age and CIN status. RESULTS: Overall, 20.7% (95% CI, 19.9-21.6%) of women were hrHPV-positive by HC2, with women with CIN having the highest rates of positivity. Prevalence decreased with increasing age among women without CIN; but, a bimodal age curve was observed among women with CIN. HPV 16 and 35 were the most common hrHPV genotypes in all age and CIN groups. HPV 45 became more frequent among older women with CIN grade 2 or 3 (CIN2,3). Younger women (17-29 years) had more multiple hrHPV genotypes overall and in each cervical disease group than older women (40-65 years). CONCLUSION: HPV 16, 35, and 45 were the leading contributors to CIN 2,3. The current HPV vaccines could significantly reduce HPV-related cervical disease; however, next generation vaccines that include HPV 35 and 45 would further reduce cervical disease in this population.en_ZA
dc.identifier.apacitationMcDonald, A. C., Denny, L., Wang, C., Tsai, W., Jr, T. C. W., & Kuhn, L. (2012). Distribution of high-risk human papillomavirus genotypes among HIV-negative women with and without cervical intraepithelial neoplasia in South Africa. <i>PLoS One</i>, http://hdl.handle.net/11427/15301en_ZA
dc.identifier.chicagocitationMcDonald, Alicia C, Lynette Denny, Chunhui Wang, Wei-Yann Tsai, Thomas C Wright Jr, and Louise Kuhn "Distribution of high-risk human papillomavirus genotypes among HIV-negative women with and without cervical intraepithelial neoplasia in South Africa." <i>PLoS One</i> (2012) http://hdl.handle.net/11427/15301en_ZA
dc.identifier.citationMcDonald, A. C., Denny, L., Wang, C., Tsai, W. Y., Wright Jr, T. C., & Kuhn, L. (2012). Distribution of high-risk human papillomavirus genotypes among HIV-negative women with and without cervical intraepithelial neoplasia in South Africa. PloS one, 7(9), e44332. doi:10.1371/journal.pone.0044332en_ZA
dc.identifier.ris TY - Journal Article AU - McDonald, Alicia C AU - Denny, Lynette AU - Wang, Chunhui AU - Tsai, Wei-Yann AU - Jr, Thomas C Wright AU - Kuhn, Louise AB - Objective Large studies describing the profile of high-risk Human papillomavirus (hrHPV) genotypes among women in sub-Saharan Africa are lacking. Here we describe the prevalence and distribution of hrHPV genotypes among HIV-negative women in South Africa, with and without cervical intraepithelial neoplasia (CIN). METHODS: We report data on 8,050 HIV-negative women, aged 17-65 years, recruited into three sequential studies undertaken in Cape Town, South Africa. Women had no history of previous cervical cancer screening. Cervical samples were tested for hrHPV DNA using the Hybrid Capture 2 (HC2) assay and all positive samples were genotyped using a PCR-based assay (Line Blot). Women underwent colposcopy and biopsy/endocervical curettage to determine CIN status. The prevalence and distribution of specific hrHPV genotypes were examined by age and CIN status. RESULTS: Overall, 20.7% (95% CI, 19.9-21.6%) of women were hrHPV-positive by HC2, with women with CIN having the highest rates of positivity. Prevalence decreased with increasing age among women without CIN; but, a bimodal age curve was observed among women with CIN. HPV 16 and 35 were the most common hrHPV genotypes in all age and CIN groups. HPV 45 became more frequent among older women with CIN grade 2 or 3 (CIN2,3). Younger women (17-29 years) had more multiple hrHPV genotypes overall and in each cervical disease group than older women (40-65 years). CONCLUSION: HPV 16, 35, and 45 were the leading contributors to CIN 2,3. The current HPV vaccines could significantly reduce HPV-related cervical disease; however, next generation vaccines that include HPV 35 and 45 would further reduce cervical disease in this population. DA - 2012 DB - OpenUCT DO - 10.1371/journal.pone.0044332 DP - University of Cape Town J1 - PLoS One LK - https://open.uct.ac.za PB - University of Cape Town PY - 2012 T1 - Distribution of high-risk human papillomavirus genotypes among HIV-negative women with and without cervical intraepithelial neoplasia in South Africa TI - Distribution of high-risk human papillomavirus genotypes among HIV-negative women with and without cervical intraepithelial neoplasia in South Africa UR - http://hdl.handle.net/11427/15301 ER - en_ZA
dc.identifier.urihttp://hdl.handle.net/11427/15301
dc.identifier.urihttp://dx.doi.org/10.1371/journal.pone.0044332
dc.identifier.vancouvercitationMcDonald AC, Denny L, Wang C, Tsai W, Jr TCW, Kuhn L. Distribution of high-risk human papillomavirus genotypes among HIV-negative women with and without cervical intraepithelial neoplasia in South Africa. PLoS One. 2012; http://hdl.handle.net/11427/15301.en_ZA
dc.language.isoengen_ZA
dc.publisherPublic Library of Scienceen_ZA
dc.publisher.departmentDepartment of Obstetrics and Gynaecologyen_ZA
dc.publisher.facultyFaculty of Health Sciencesen_ZA
dc.publisher.institutionUniversity of Cape Town
dc.rightsThis is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.en_ZA
dc.rights.holder© McDonald et alen_ZA
dc.rights.urihttp://creativecommons.org/licenses/by/4.0en_ZA
dc.sourcePLoS Oneen_ZA
dc.source.urihttp://journals.plos.org/plosoneen_ZA
dc.subject.otherHuman papillomavirus infectionen_ZA
dc.subject.otherCervical canceren_ZA
dc.subject.otherPhylogeographyen_ZA
dc.subject.otherHPV-18en_ZA
dc.subject.otherAfricaen_ZA
dc.subject.otherHuman papillomavirusen_ZA
dc.subject.otherHPV-45en_ZA
dc.subject.otherCancer screeningen_ZA
dc.titleDistribution of high-risk human papillomavirus genotypes among HIV-negative women with and without cervical intraepithelial neoplasia in South Africaen_ZA
dc.typeJournal Articleen_ZA
uct.type.filetypeText
uct.type.filetypeImage
uct.type.publicationResearchen_ZA
uct.type.resourceArticleen_ZA
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