The predominant polyphenol in the leaves of the resurrection plant Myrothamnus flabellifolius, 3,4,5 tri-O-galloylquinic acid, protects membranes against desiccation and free radical-induced oxidation

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dc.contributor.authorMoore, John P
dc.contributor.authorWestall, Kim L
dc.contributor.authorRavenscroft, Neil
dc.contributor.authorFarrant, Jill M
dc.contributor.authorLindsey, George G
dc.contributor.authorBrandt, Wolf F
dc.date.accessioned2016-07-29T16:14:08Z
dc.date.available2016-07-29T16:14:08Z
dc.date.issued2005
dc.date.updated2016-07-29T15:23:42Z
dc.description.abstractThe predominant (>90%) low-molecular-mass polyphenol was isolated from the leaves of the resurrection plant Myrothamnus flabellifolius and identified to be 3,4,5 tri-O-galloylquinic acid using 1 H and 13C one- and two-dimensional NMR spectroscopy. The structure was confirmed by mass spectrometric analysis. This compound was present at high concentrations, 44%(by weight) in hydrated leaves and 74% (by weight) in dehydrated leaves. Electron microscopy of leaf material fixed with glutaraldehyde and caffeine demonstrated that the polyphenols were localized in large vacuoles in both hydrated and dehydrated leaves. 3,4,5 Tri-O-galloylquinic acid was shown to stabilize an artificial membrane system, liposomes, against desiccation if the polyphenol concentration was between 1 and 2 µg/µg phospholipid. The phase transition of these liposomes observed at 46 ◦C was markedly diminished by the presence of 3,4,5 tri-O-galloylquinic acid, suggesting that the presence of the polyphenol maintained the membranes in the liquid crystalline phase at physiological temperatures. 3,4,5 Tri-O-galloylquinic acid was also shown to protect linoleic acid against free radical-induced oxidation.en_ZA
dc.identifierhttp://dx.doi.org/10.1042/BJ20040499
dc.identifier.apacitationMoore, J. P., Westall, K. L., Ravenscroft, N., Farrant, J. M., Lindsey, G. G., & Brandt, W. F. (2005). The predominant polyphenol in the leaves of the resurrection plant Myrothamnus flabellifolius, 3,4,5 tri-O-galloylquinic acid, protects membranes against desiccation and free radical-induced oxidation. <i>Biochemical Journal</i>, http://hdl.handle.net/11427/21072en_ZA
dc.identifier.chicagocitationMoore, John P, Kim L Westall, Neil Ravenscroft, Jill M Farrant, George G Lindsey, and Wolf F Brandt "The predominant polyphenol in the leaves of the resurrection plant Myrothamnus flabellifolius, 3,4,5 tri-O-galloylquinic acid, protects membranes against desiccation and free radical-induced oxidation." <i>Biochemical Journal</i> (2005) http://hdl.handle.net/11427/21072en_ZA
dc.identifier.citationMoore, J. P., Westall, K. L., Ravenscroft, N., Farrant, J. M., Lindsey, G. G., & Brandt, W. F. (2005). The predominant polyphenol in the leaves of the resurrection plant Myrothamnus flabellifolius, 3, 4, 5 tri-O-galloylquinic acid, protects membranes against desiccation and free radical-induced oxidation. Biochemical Journal, 385(1), 301-308.en_ZA
dc.identifier.issn0264-6021en_ZA
dc.identifier.ris TY - Journal Article AU - Moore, John P AU - Westall, Kim L AU - Ravenscroft, Neil AU - Farrant, Jill M AU - Lindsey, George G AU - Brandt, Wolf F AB - The predominant (>90%) low-molecular-mass polyphenol was isolated from the leaves of the resurrection plant Myrothamnus flabellifolius and identified to be 3,4,5 tri-O-galloylquinic acid using 1 H and 13C one- and two-dimensional NMR spectroscopy. The structure was confirmed by mass spectrometric analysis. This compound was present at high concentrations, 44%(by weight) in hydrated leaves and 74% (by weight) in dehydrated leaves. Electron microscopy of leaf material fixed with glutaraldehyde and caffeine demonstrated that the polyphenols were localized in large vacuoles in both hydrated and dehydrated leaves. 3,4,5 Tri-O-galloylquinic acid was shown to stabilize an artificial membrane system, liposomes, against desiccation if the polyphenol concentration was between 1 and 2 µg/µg phospholipid. The phase transition of these liposomes observed at 46 ◦C was markedly diminished by the presence of 3,4,5 tri-O-galloylquinic acid, suggesting that the presence of the polyphenol maintained the membranes in the liquid crystalline phase at physiological temperatures. 3,4,5 Tri-O-galloylquinic acid was also shown to protect linoleic acid against free radical-induced oxidation. DA - 2005 DB - OpenUCT DP - University of Cape Town J1 - Biochemical Journal LK - https://open.uct.ac.za PB - University of Cape Town PY - 2005 SM - 0264-6021 T1 - The predominant polyphenol in the leaves of the resurrection plant Myrothamnus flabellifolius, 3,4,5 tri-O-galloylquinic acid, protects membranes against desiccation and free radical-induced oxidation TI - The predominant polyphenol in the leaves of the resurrection plant Myrothamnus flabellifolius, 3,4,5 tri-O-galloylquinic acid, protects membranes against desiccation and free radical-induced oxidation UR - http://hdl.handle.net/11427/21072 ER - en_ZA
dc.identifier.urihttp://hdl.handle.net/11427/21072
dc.identifier.vancouvercitationMoore JP, Westall KL, Ravenscroft N, Farrant JM, Lindsey GG, Brandt WF. The predominant polyphenol in the leaves of the resurrection plant Myrothamnus flabellifolius, 3,4,5 tri-O-galloylquinic acid, protects membranes against desiccation and free radical-induced oxidation. Biochemical Journal. 2005; http://hdl.handle.net/11427/21072.en_ZA
dc.languageengen_ZA
dc.publisherPortland Pressen_ZA
dc.publisher.institutionUniversity of Cape Town
dc.sourceBiochemical Journalen_ZA
dc.source.urihttp://www.biochemj.org/
dc.subject.otherDesiccation
dc.subject.otherLiposome
dc.subject.otherMS
dc.subject.otherMyrothamnus flabellifolius
dc.subject.otherNMR spectroscopy
dc.titleThe predominant polyphenol in the leaves of the resurrection plant Myrothamnus flabellifolius, 3,4,5 tri-O-galloylquinic acid, protects membranes against desiccation and free radical-induced oxidationen_ZA
dc.typeJournal Articleen_ZA
uct.type.filetypeText
uct.type.filetypeImage
uct.type.publicationResearchen_ZA
uct.type.resourceArticleen_ZA
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