Association between ethnicity and obesity with high-density lipoprotein (HDL) function and subclass distribution

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dc.contributor.authorWoudberg, Nicholas J
dc.contributor.authorGoedecke, Julia H
dc.contributor.authorBlackhurst, Dee
dc.contributor.authorFrias, Miguel
dc.contributor.authorJames, Richard
dc.contributor.authorOpie, Lionel H
dc.contributor.authorLecour, Sandrine
dc.date.accessioned2021-10-08T07:08:18Z
dc.date.available2021-10-08T07:08:18Z
dc.date.issued2016
dc.description.abstractAbstract Background Obesity and low high-density lipoprotein-cholesterol (HDL-C) levels are associated with cardiovascular risk. Surprisingly, despite a greater prevalence of obesity and lower HDL concentrations than white women, black South African women are relatively protected against ischaemic heart disease. Methods We investigated whether this apparent discrepancy may be related to different HDL function and subclass distribution in black and white, normal-weight and obese South African women (n = 40). HDL functionality was assessed by measuring paraoxonase (PON) activity, platelet activating factor acetylhydrolase (PAF-AH) activity, Oxygen Radical Absorbance Capacity (ORAC) and quantification of the expression of vascular cell adhesion molecule in endothelial cells. PON-1 and PAF-AH expression was determined in isolated HDL and serum using Western blotting. Levels of large, intermediate and small HDL subclasses were measured using the Lipoprint® system. Results PON activity was lower in white compared to black women (0.49 ± 0.09 U/L vs 0.78 ± 0.10 U/L, p < 0.05), regardless of PON-1 protein levels. Obese black women had lower PAF-AH activity (9.34 ± 1.15 U/L vs 13.89 ± 1.21 U/L, p <0.05) and HDL-associated PAF-AH expression compared to obese white women. Compared to normal-weight women, obese women had lower large HDL, greater intermediate and small HDL; an effect that was more pronounced in white women than black women. There were no differences in antioxidant capacity or anti-inflammatory function across groups. Conclusions Our data show that both obesity and ethnicity are associated with differences in HDL functionality, while obesity was associated with decreases in large HDL subclass distribution. Measuring HDL functionality and subclass may, therefore, be important factors to consider when assessing cardiovascular risk.
dc.identifier.apacitationWoudberg, N. J., Goedecke, J. H., Blackhurst, D., Frias, M., James, R., Opie, L. H., & Lecour, S. (2016). Association between ethnicity and obesity with high-density lipoprotein (HDL) function and subclass distribution. <i>Lipids in Health and Disease</i>, 15(1), 174 - 177. http://hdl.handle.net/11427/34548en_ZA
dc.identifier.chicagocitationWoudberg, Nicholas J, Julia H Goedecke, Dee Blackhurst, Miguel Frias, Richard James, Lionel H Opie, and Sandrine Lecour "Association between ethnicity and obesity with high-density lipoprotein (HDL) function and subclass distribution." <i>Lipids in Health and Disease</i> 15, 1. (2016): 174 - 177. http://hdl.handle.net/11427/34548en_ZA
dc.identifier.citationWoudberg, N.J., Goedecke, J.H., Blackhurst, D., Frias, M., James, R., Opie, L.H. & Lecour, S. 2016. Association between ethnicity and obesity with high-density lipoprotein (HDL) function and subclass distribution. <i>Lipids in Health and Disease.</i> 15(1):174 - 177. http://hdl.handle.net/11427/34548en_ZA
dc.identifier.issn1476-511X
dc.identifier.ris TY - Journal Article AU - Woudberg, Nicholas J AU - Goedecke, Julia H AU - Blackhurst, Dee AU - Frias, Miguel AU - James, Richard AU - Opie, Lionel H AU - Lecour, Sandrine AB - Abstract Background Obesity and low high-density lipoprotein-cholesterol (HDL-C) levels are associated with cardiovascular risk. Surprisingly, despite a greater prevalence of obesity and lower HDL concentrations than white women, black South African women are relatively protected against ischaemic heart disease. Methods We investigated whether this apparent discrepancy may be related to different HDL function and subclass distribution in black and white, normal-weight and obese South African women (n = 40). HDL functionality was assessed by measuring paraoxonase (PON) activity, platelet activating factor acetylhydrolase (PAF-AH) activity, Oxygen Radical Absorbance Capacity (ORAC) and quantification of the expression of vascular cell adhesion molecule in endothelial cells. PON-1 and PAF-AH expression was determined in isolated HDL and serum using Western blotting. Levels of large, intermediate and small HDL subclasses were measured using the Lipoprint® system. Results PON activity was lower in white compared to black women (0.49 ± 0.09 U/L vs 0.78 ± 0.10 U/L, p < 0.05), regardless of PON-1 protein levels. Obese black women had lower PAF-AH activity (9.34 ± 1.15 U/L vs 13.89 ± 1.21 U/L, p <0.05) and HDL-associated PAF-AH expression compared to obese white women. Compared to normal-weight women, obese women had lower large HDL, greater intermediate and small HDL; an effect that was more pronounced in white women than black women. There were no differences in antioxidant capacity or anti-inflammatory function across groups. Conclusions Our data show that both obesity and ethnicity are associated with differences in HDL functionality, while obesity was associated with decreases in large HDL subclass distribution. Measuring HDL functionality and subclass may, therefore, be important factors to consider when assessing cardiovascular risk. DA - 2016 DB - OpenUCT DP - University of Cape Town IS - 1 J1 - Lipids in Health and Disease LK - https://open.uct.ac.za PY - 2016 SM - 1476-511X T1 - Association between ethnicity and obesity with high-density lipoprotein (HDL) function and subclass distribution TI - Association between ethnicity and obesity with high-density lipoprotein (HDL) function and subclass distribution UR - http://hdl.handle.net/11427/34548 ER - en_ZA
dc.identifier.urihttp://hdl.handle.net/11427/34548
dc.identifier.vancouvercitationWoudberg NJ, Goedecke JH, Blackhurst D, Frias M, James R, Opie LH, et al. Association between ethnicity and obesity with high-density lipoprotein (HDL) function and subclass distribution. Lipids in Health and Disease. 2016;15(1):174 - 177. http://hdl.handle.net/11427/34548.en_ZA
dc.language.isoeng
dc.publisher.departmentDepartment of Medicine
dc.publisher.facultyFaculty of Health Sciences
dc.sourceLipids in Health and Disease
dc.source.journalissue1
dc.source.journalvolume15
dc.source.pagination174 - 177
dc.source.urihttps://dx.doi.org/10.1186/s12944-016-0257-9
dc.subject.otherPLA2G7
dc.subject.otherPON1
dc.subject.otherObesity
dc.subject.otherHeart Diseases
dc.subject.otherPons
dc.subject.otherSerum
dc.subject.otherEndothelial Cells
dc.subject.otherVascular Cell Adhesion Molecule-1
dc.subject.otherAnti-Inflammatory Agents
dc.subject.otherPlatelet Activating Factor
dc.subject.otherProteins
dc.subject.otherCholesterol, LDL
dc.subject.other1-Alkyl-2-acetylglycerophosphocholine Esterase
dc.subject.otherLipoproteins, HDL
dc.subject.otherAntioxidants
dc.subject.otherOxygen
dc.subject.otherAryldialkylphosphatase
dc.subject.otherBlotting, Western
dc.subject.otherRisk
dc.subject.otherPrevalence
dc.subject.otherHomo sapiens
dc.subject.otherLipidology
dc.subject.otherMedical Biochemistry
dc.subject.otherClinical Nutrition
dc.titleAssociation between ethnicity and obesity with high-density lipoprotein (HDL) function and subclass distribution
dc.typeJournal Article
uct.type.publicationResearch
uct.type.resourceJournal Article
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