Analysis of virulence factors and antibiotic resistance genes in group B streptococcus from clinical samples

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dc.contributor.authorMudzana, Raymond
dc.contributor.authorMavenyengwa, Rooyen T
dc.contributor.authorGudza-Mugabe, Muchaneta
dc.date.accessioned2021-10-12T07:59:09Z
dc.date.available2021-10-12T07:59:09Z
dc.date.issued2021-01-28
dc.date.updated2021-01-31T04:13:38Z
dc.description.abstractBackground Streptococcus agalacticae (Group B Streptococcus, GBS) is one of the most important causative agents of serious infections among neonates. This study was carried out to identify antibiotic resistance and virulence genes associated with GBS isolated from pregnant women. Methods A total of 43 GBS isolates were obtained from 420 vaginal samples collected from HIV positive and negative women who were 13–35 weeks pregnant attending Antenatal Care at Chitungwiza and Harare Central Hospitals in Zimbabwe. Identification tests of GBS isolates was done using standard bacteriological methods and molecular identification testing. Antibiotic susceptibility testing was done using the modified Kirby-Bauer method and E-test strips. The boiling method was used to extract DNA and Polymerase Chain Reaction (PCR) was used to screen for 13 genes. Data was fed into SPSS 24.0. Results Nine distinct virulence gene profiles were identified and hly-scpB-bca-rib 37.2% (16/43) was common. The virulence genes identified were namely hly 97.8% (42/43), scpB 90.1% (39/43), bca 86.0% (37/43), rib 69.8% (30/43) and bac 11.6% (5/43). High resistance to tetracycline 97.7% (42/43) was reported followed by 72.1% (31/43) cefazolin, 69.8% (30/43) penicillin G, 58.1% (25/43) ampicillin, 55.8% (24/43) clindamycin, 46.5% (20/43) ceftriaxone, 34.9% (15/43) chloramphenicol, and 30.2% (13/43) for both erythromycin and vancomycin using disk diffusion. Antibiotic resistance genes among the resistant and intermediate-resistant isolates showed high frequencies for tetM 97.6% (41/42) and low frequencies for ermB 34.5% (10/29), ermTR 10.3% (3/29), mefA 3.4% (1/29), tetO 2.4% (1/42) and linB 0% (0/35). The atr housekeeping gene yielded 100% (43/43) positive results, whilst the mobile genetic element IS1548 yielded 9.3% (4/43). Conclusion The study showed high prevalence of hly, scpB, bca and rib virulence genes in S. agalactiae strains isolated from pregnant women. Tetracycline resistance was predominantly caused by the tetM gene, whilst macrolide resistance was predominantly due to the presence of erm methylase, with the ermB gene being more prevalent. Multi-drug resistance coupled with the recovery of resistant isolates to antimicrobial agents such as penicillins indicates the importance of GBS surveillance and susceptibility tests. It was also observed that in vitro phenotypic resistance is not always accurately predicted by resistance genotypes.en_US
dc.identifier.apacitationMudzana, R., Mavenyengwa, R. T., & Gudza-Mugabe, M. (2021). Analysis of virulence factors and antibiotic resistance genes in group B streptococcus from clinical samples. <i>BMC Infectious Diseases</i>, 21(Article number: 125), http://hdl.handle.net/11427/35187en_ZA
dc.identifier.chicagocitationMudzana, Raymond, Rooyen T Mavenyengwa, and Muchaneta Gudza-Mugabe "Analysis of virulence factors and antibiotic resistance genes in group B streptococcus from clinical samples." <i>BMC Infectious Diseases</i> 21, Article number: 125. (2021) http://hdl.handle.net/11427/35187en_ZA
dc.identifier.citationMudzana, R., Mavenyengwa, R.T. & Gudza-Mugabe, M. 2021. Analysis of virulence factors and antibiotic resistance genes in group B streptococcus from clinical samples. <i>BMC Infectious Diseases.</i> 21(Article number: 125) http://hdl.handle.net/11427/35187en_ZA
dc.identifier.ris TY - Journal Article AU - Mudzana, Raymond AU - Mavenyengwa, Rooyen T AU - Gudza-Mugabe, Muchaneta AB - Background Streptococcus agalacticae (Group B Streptococcus, GBS) is one of the most important causative agents of serious infections among neonates. This study was carried out to identify antibiotic resistance and virulence genes associated with GBS isolated from pregnant women. Methods A total of 43 GBS isolates were obtained from 420 vaginal samples collected from HIV positive and negative women who were 13–35 weeks pregnant attending Antenatal Care at Chitungwiza and Harare Central Hospitals in Zimbabwe. Identification tests of GBS isolates was done using standard bacteriological methods and molecular identification testing. Antibiotic susceptibility testing was done using the modified Kirby-Bauer method and E-test strips. The boiling method was used to extract DNA and Polymerase Chain Reaction (PCR) was used to screen for 13 genes. Data was fed into SPSS 24.0. Results Nine distinct virulence gene profiles were identified and hly-scpB-bca-rib 37.2% (16/43) was common. The virulence genes identified were namely hly 97.8% (42/43), scpB 90.1% (39/43), bca 86.0% (37/43), rib 69.8% (30/43) and bac 11.6% (5/43). High resistance to tetracycline 97.7% (42/43) was reported followed by 72.1% (31/43) cefazolin, 69.8% (30/43) penicillin G, 58.1% (25/43) ampicillin, 55.8% (24/43) clindamycin, 46.5% (20/43) ceftriaxone, 34.9% (15/43) chloramphenicol, and 30.2% (13/43) for both erythromycin and vancomycin using disk diffusion. Antibiotic resistance genes among the resistant and intermediate-resistant isolates showed high frequencies for tetM 97.6% (41/42) and low frequencies for ermB 34.5% (10/29), ermTR 10.3% (3/29), mefA 3.4% (1/29), tetO 2.4% (1/42) and linB 0% (0/35). The atr housekeeping gene yielded 100% (43/43) positive results, whilst the mobile genetic element IS1548 yielded 9.3% (4/43). Conclusion The study showed high prevalence of hly, scpB, bca and rib virulence genes in S. agalactiae strains isolated from pregnant women. Tetracycline resistance was predominantly caused by the tetM gene, whilst macrolide resistance was predominantly due to the presence of erm methylase, with the ermB gene being more prevalent. Multi-drug resistance coupled with the recovery of resistant isolates to antimicrobial agents such as penicillins indicates the importance of GBS surveillance and susceptibility tests. It was also observed that in vitro phenotypic resistance is not always accurately predicted by resistance genotypes. DA - 2021-01-28 DB - OpenUCT DP - University of Cape Town IS - Article number: 125 J1 - BMC Infectious Diseases KW - Group B streptococcus KW - Virulence genes KW - Antibiotic resistance genes KW - PCR KW - Zimbabwe LK - https://open.uct.ac.za PY - 2021 T1 - Analysis of virulence factors and antibiotic resistance genes in group B streptococcus from clinical samples TI - Analysis of virulence factors and antibiotic resistance genes in group B streptococcus from clinical samples UR - http://hdl.handle.net/11427/35187 ER - en_ZA
dc.identifier.urihttps://doi.org/10.1186/s12879-021-05820-6
dc.identifier.urihttp://hdl.handle.net/11427/35187
dc.identifier.vancouvercitationMudzana R, Mavenyengwa RT, Gudza-Mugabe M. Analysis of virulence factors and antibiotic resistance genes in group B streptococcus from clinical samples. BMC Infectious Diseases. 2021;21(Article number: 125) http://hdl.handle.net/11427/35187.en_ZA
dc.language.isoenen_US
dc.language.rfc3066en
dc.publisher.departmentInstitute of Infectious Disease and Molecular Medicineen_US
dc.publisher.facultyFaculty of Health Sciencesen_US
dc.rights.holderThe Author(s)
dc.rights.urihttp://creativecommons.org/licenses/by/4.0/en_US
dc.sourceBMC Infectious Diseasesen_US
dc.source.journalissueArticle number: 125en_US
dc.source.journalvolume21en_US
dc.source.urihttps://bmcinfectdis.biomedcentral.com/
dc.subjectGroup B streptococcusen_US
dc.subjectVirulence genesen_US
dc.subjectAntibiotic resistance genesen_US
dc.subjectPCRen_US
dc.subjectZimbabween_US
dc.titleAnalysis of virulence factors and antibiotic resistance genes in group B streptococcus from clinical samplesen_US
dc.typeJournal Articleen_US
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