Apoliprotein B metabolism in hamster livers, studied in vitro

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dc.contributor.advisorGevers, Wielanden_ZA
dc.contributor.authorHayward, Nicola Margareten_ZA
dc.date.accessioned2018-01-31T13:47:24Z
dc.date.available2018-01-31T13:47:24Z
dc.date.issued1990en_ZA
dc.description.abstractThis study aimed to investigate lipoprotein metabolism in male hamsters fed diets considered to be atherogenic in humans. Livers from adult male hamsters were selected to study aspects of apolipoprotein B metabolism. Isolated hepatocytes in suspension were compared with those maintained under tissue culture conditions. Liver slices were also prepared and compared with isolated suspended hepatocytes. Freshly prepared hepatocytes from the animals were incubated with radiolabelled precursors in suspension, or they were maintained under tissue culture conditions; liver slices were also investigated. The rates of total protein synthesis were of the same order in each of these systems, but protein secretion was impaired in liver slices, probably as a result of diffusion problems associated with the altered architecture of the sliced tissue. Albumin constituted 40 - 50% of the secreted proteins in each system. The rates of VLDL synthesis were increased in cells and slices prepared from animals previously fed sucrose- or fat-rich diets, but the secretion of VLDL was inhibited when diets contained unsaturated fat. The overall synthesis of apolipoprotein B was enhanced by fat-feeding; in the case of suspended hepatocytes, secretion of this protein was decreased when the preceding diet contained fats that were unsaturated; while in the case of liver slices, secretion was paradoxically enhanced. Apolipoprotein B was not degraded at significant rates in hepatocytes prepared from either control or fat-fed hamsters.en_ZA
dc.identifier.apacitationHayward, N. M. (1990). <i>Apoliprotein B metabolism in hamster livers, studied in vitro</i>. (Thesis). University of Cape Town ,Faculty of Health Sciences ,Division of Medical Biochemistry & Structural Biology. Retrieved from http://hdl.handle.net/11427/27176en_ZA
dc.identifier.chicagocitationHayward, Nicola Margaret. <i>"Apoliprotein B metabolism in hamster livers, studied in vitro."</i> Thesis., University of Cape Town ,Faculty of Health Sciences ,Division of Medical Biochemistry & Structural Biology, 1990. http://hdl.handle.net/11427/27176en_ZA
dc.identifier.citationHayward, N. 1990. Apoliprotein B metabolism in hamster livers, studied in vitro. University of Cape Town.en_ZA
dc.identifier.ris TY - Thesis / Dissertation AU - Hayward, Nicola Margaret AB - This study aimed to investigate lipoprotein metabolism in male hamsters fed diets considered to be atherogenic in humans. Livers from adult male hamsters were selected to study aspects of apolipoprotein B metabolism. Isolated hepatocytes in suspension were compared with those maintained under tissue culture conditions. Liver slices were also prepared and compared with isolated suspended hepatocytes. Freshly prepared hepatocytes from the animals were incubated with radiolabelled precursors in suspension, or they were maintained under tissue culture conditions; liver slices were also investigated. The rates of total protein synthesis were of the same order in each of these systems, but protein secretion was impaired in liver slices, probably as a result of diffusion problems associated with the altered architecture of the sliced tissue. Albumin constituted 40 - 50% of the secreted proteins in each system. The rates of VLDL synthesis were increased in cells and slices prepared from animals previously fed sucrose- or fat-rich diets, but the secretion of VLDL was inhibited when diets contained unsaturated fat. The overall synthesis of apolipoprotein B was enhanced by fat-feeding; in the case of suspended hepatocytes, secretion of this protein was decreased when the preceding diet contained fats that were unsaturated; while in the case of liver slices, secretion was paradoxically enhanced. Apolipoprotein B was not degraded at significant rates in hepatocytes prepared from either control or fat-fed hamsters. DA - 1990 DB - OpenUCT DP - University of Cape Town LK - https://open.uct.ac.za PB - University of Cape Town PY - 1990 T1 - Apoliprotein B metabolism in hamster livers, studied in vitro TI - Apoliprotein B metabolism in hamster livers, studied in vitro UR - http://hdl.handle.net/11427/27176 ER - en_ZA
dc.identifier.urihttp://hdl.handle.net/11427/27176
dc.identifier.vancouvercitationHayward NM. Apoliprotein B metabolism in hamster livers, studied in vitro. [Thesis]. University of Cape Town ,Faculty of Health Sciences ,Division of Medical Biochemistry & Structural Biology, 1990 [cited yyyy month dd]. Available from: http://hdl.handle.net/11427/27176en_ZA
dc.language.isoengen_ZA
dc.publisher.departmentDivision of Medical Biochemistry and Structural Biology
dc.publisher.facultyFaculty of Health Sciencesen_ZA
dc.publisher.institutionUniversity of Cape Town
dc.subject.otherHamstersen_ZA
dc.subject.otherApolipoproteins B - metabolismen_ZA
dc.subject.otherLiver - Metabolismen_ZA
dc.titleApoliprotein B metabolism in hamster livers, studied in vitroen_ZA
dc.typeMaster Thesis
dc.type.qualificationlevelMasters
dc.type.qualificationnameMSc (Med)en_ZA
uct.type.filetypeText
uct.type.filetypeImage
uct.type.publicationResearchen_ZA
uct.type.resourceThesisen_ZA
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