Response of the leaf cell wall to desiccation in the resurrection plant Myrothamnus flabellifolius

dc.contributor.authorMoore, John P
dc.contributor.authorNguema-Ona, Eric
dc.contributor.authorChevalier, Laurence
dc.contributor.authorLindsey, George G
dc.contributor.authorBrandt, Wolf F
dc.contributor.authorLerouge, Patrice
dc.contributor.authorFarrant, Jill M
dc.contributor.authorDriouich, Azeddine
dc.date.accessioned2016-07-30T19:31:08Z
dc.date.available2016-07-30T19:31:08Z
dc.date.issued2006
dc.date.updated2016-07-29T13:51:22Z
dc.description.abstractThe Myrothamnus flabellifolius leaf cell wall and its response to desiccation were investigated using electron microscopic, biochemical, and immunocytochemical techniques. Electron microscopy revealed desiccation-induced cell wall folding in the majority of mesophyll and epidermal cells. Thick-walled vascular tissue and sclerenchymous ribs did not fold and supported the surrounding tissue, thereby limiting the extent of leaf shrinkage and allowing leaf morphology to be rapidly regained upon rehydration. Isolated cell walls from hydrated and desiccated M. flabellifolius leaves were fractionated into their constituent polymers and the resulting fractions were analyzed for monosaccharide content. Significant differences between hydrated and desiccated states were observed in the water-soluble buffer extract, pectin fractions, and the arabinogalactan protein-rich extract. A marked increase in galacturonic acid was found in the alkali-insoluble pectic fraction. Xyloglucan structure was analyzed and shown to be of the standard dicotyledonous pattern. Immunocytochemical analysis determined the cellular location of the various epitopes associated with cell wall components, including pectin, xyloglucan, and arabinogalactan proteins, in hydrated and desiccated leaf tissue. The most striking observation was a constitutively present high concentration of arabinose, which was associated with pectin, presumably in the form of arabinan polymers. We propose that the arabinan-rich leaf cell wall of M. flabellifolius possesses the necessary structural properties to be able to undergo repeated periods of desiccation and rehydration.en_ZA
dc.identifierhttp://dx.doi.org/10.1104/pp.106.077701
dc.identifier.apacitationMoore, J. P., Nguema-Ona, E., Chevalier, L., Lindsey, G. G., Brandt, W. F., Lerouge, P., ... Driouich, A. (2006). Response of the leaf cell wall to desiccation in the resurrection plant Myrothamnus flabellifolius. <i>Plant Physiology</i>, http://hdl.handle.net/11427/21077en_ZA
dc.identifier.chicagocitationMoore, John P, Eric Nguema-Ona, Laurence Chevalier, George G Lindsey, Wolf F Brandt, Patrice Lerouge, Jill M Farrant, and Azeddine Driouich "Response of the leaf cell wall to desiccation in the resurrection plant Myrothamnus flabellifolius." <i>Plant Physiology</i> (2006) http://hdl.handle.net/11427/21077en_ZA
dc.identifier.citationMoore, J. P., Nguema-Ona, E., Chevalier, L., Lindsey, G. G., Brandt, W. F., Lerouge, P., ... & Driouich, A. (2006). Response of the leaf cell wall to desiccation in the resurrection plant Myrothamnus flabellifolius. Plant Physiology, 141(2), 651-662.en_ZA
dc.identifier.issn0032-0889en_ZA
dc.identifier.ris TY - Journal Article AU - Moore, John P AU - Nguema-Ona, Eric AU - Chevalier, Laurence AU - Lindsey, George G AU - Brandt, Wolf F AU - Lerouge, Patrice AU - Farrant, Jill M AU - Driouich, Azeddine AB - The Myrothamnus flabellifolius leaf cell wall and its response to desiccation were investigated using electron microscopic, biochemical, and immunocytochemical techniques. Electron microscopy revealed desiccation-induced cell wall folding in the majority of mesophyll and epidermal cells. Thick-walled vascular tissue and sclerenchymous ribs did not fold and supported the surrounding tissue, thereby limiting the extent of leaf shrinkage and allowing leaf morphology to be rapidly regained upon rehydration. Isolated cell walls from hydrated and desiccated M. flabellifolius leaves were fractionated into their constituent polymers and the resulting fractions were analyzed for monosaccharide content. Significant differences between hydrated and desiccated states were observed in the water-soluble buffer extract, pectin fractions, and the arabinogalactan protein-rich extract. A marked increase in galacturonic acid was found in the alkali-insoluble pectic fraction. Xyloglucan structure was analyzed and shown to be of the standard dicotyledonous pattern. Immunocytochemical analysis determined the cellular location of the various epitopes associated with cell wall components, including pectin, xyloglucan, and arabinogalactan proteins, in hydrated and desiccated leaf tissue. The most striking observation was a constitutively present high concentration of arabinose, which was associated with pectin, presumably in the form of arabinan polymers. We propose that the arabinan-rich leaf cell wall of M. flabellifolius possesses the necessary structural properties to be able to undergo repeated periods of desiccation and rehydration. DA - 2006 DB - OpenUCT DP - University of Cape Town J1 - Plant Physiology LK - https://open.uct.ac.za PB - University of Cape Town PY - 2006 SM - 0032-0889 T1 - Response of the leaf cell wall to desiccation in the resurrection plant Myrothamnus flabellifolius TI - Response of the leaf cell wall to desiccation in the resurrection plant Myrothamnus flabellifolius UR - http://hdl.handle.net/11427/21077 ER - en_ZA
dc.identifier.urihttp://hdl.handle.net/11427/21077
dc.identifier.vancouvercitationMoore JP, Nguema-Ona E, Chevalier L, Lindsey GG, Brandt WF, Lerouge P, et al. Response of the leaf cell wall to desiccation in the resurrection plant Myrothamnus flabellifolius. Plant Physiology. 2006; http://hdl.handle.net/11427/21077.en_ZA
dc.languageengen_ZA
dc.publisherAmerican Society of Plant Biologistsen_ZA
dc.publisher.institutionUniversity of Cape Town
dc.sourcePlant Physiologyen_ZA
dc.source.urihttp://www.plantphysiol.org/
dc.titleResponse of the leaf cell wall to desiccation in the resurrection plant Myrothamnus flabellifoliusen_ZA
dc.typeJournal Articleen_ZA
uct.type.filetypeText
uct.type.filetypeImage
uct.type.publicationResearchen_ZA
uct.type.resourceArticleen_ZA
Files
Original bundle
Now showing 1 - 1 of 1
Loading...
Thumbnail Image
Name:
Moore_Response_leaf_cell_2006.pdf
Size:
482.79 KB
Format:
Adobe Portable Document Format
Description:
License bundle
Now showing 1 - 1 of 1
Loading...
Thumbnail Image
Name:
license.txt
Size:
1.72 KB
Format:
Item-specific license agreed upon to submission
Description:
Collections