Investigating the by-stander effect of Hypericin induced photodynamic therapy on human skin cells

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dc.contributor.advisorDavids, Lester Men_ZA
dc.contributor.authorPopovic, Anaen_ZA
dc.date.accessioned2015-07-01T09:01:24Z
dc.date.available2015-07-01T09:01:24Z
dc.date.issued2014en_ZA
dc.descriptionIncludes bibliographical references.en_ZA
dc.description.abstractSkin cancer is the most common cancer worldwide, and its incidence rate in South Africa is increasing. Photodynamic therapy (PDT) has been shown to be an effective treatment modality, through topical administration, for treatment of non-melanoma skin cancers. Our group investigates hypericin-induced PDT (HYP-PDT) for the treatment of both non-melanoma and melanoma skin cancers. However, a prerequisite for effective cancer treatments is efficient and selective targeting of the tumoral cells with minimal collateral damage to the surrounding normal cells, as it is well know that cancer therapies have bystander effects on normal cells in the body, often causing undesirable side effects. PDT can induce a bystander effect, defined as indirect damaged induced into adjacent cells either via intercellular gap junctions or via diffusible ROS released in the microenvironment. It is therefore important to know the effects of HYP-PDT on the normal cell population surrounding the non-melanoma skin cancer or melanoma tumor. The aim of this project was to investigate the cellular and molecular effects of HYP-PDT on normal primary human keratinocytes (Kc), melanocytes (Mc) and fibroblasts (Fb) in an in vitro tissue culture model thus representing both the epidermal and dermal cellular compartments of human skin.en_ZA
dc.identifier.apacitationPopovic, A. (2014). <i>Investigating the by-stander effect of Hypericin induced photodynamic therapy on human skin cells</i>. (Thesis). University of Cape Town ,Faculty of Health Sciences ,Department of Human Biology. Retrieved from http://hdl.handle.net/11427/13240en_ZA
dc.identifier.chicagocitationPopovic, Ana. <i>"Investigating the by-stander effect of Hypericin induced photodynamic therapy on human skin cells."</i> Thesis., University of Cape Town ,Faculty of Health Sciences ,Department of Human Biology, 2014. http://hdl.handle.net/11427/13240en_ZA
dc.identifier.citationPopovic, A. 2014. Investigating the by-stander effect of Hypericin induced photodynamic therapy on human skin cells. University of Cape Town.en_ZA
dc.identifier.ris TY - Thesis / Dissertation AU - Popovic, Ana AB - Skin cancer is the most common cancer worldwide, and its incidence rate in South Africa is increasing. Photodynamic therapy (PDT) has been shown to be an effective treatment modality, through topical administration, for treatment of non-melanoma skin cancers. Our group investigates hypericin-induced PDT (HYP-PDT) for the treatment of both non-melanoma and melanoma skin cancers. However, a prerequisite for effective cancer treatments is efficient and selective targeting of the tumoral cells with minimal collateral damage to the surrounding normal cells, as it is well know that cancer therapies have bystander effects on normal cells in the body, often causing undesirable side effects. PDT can induce a bystander effect, defined as indirect damaged induced into adjacent cells either via intercellular gap junctions or via diffusible ROS released in the microenvironment. It is therefore important to know the effects of HYP-PDT on the normal cell population surrounding the non-melanoma skin cancer or melanoma tumor. The aim of this project was to investigate the cellular and molecular effects of HYP-PDT on normal primary human keratinocytes (Kc), melanocytes (Mc) and fibroblasts (Fb) in an in vitro tissue culture model thus representing both the epidermal and dermal cellular compartments of human skin. DA - 2014 DB - OpenUCT DP - University of Cape Town LK - https://open.uct.ac.za PB - University of Cape Town PY - 2014 T1 - Investigating the by-stander effect of Hypericin induced photodynamic therapy on human skin cells TI - Investigating the by-stander effect of Hypericin induced photodynamic therapy on human skin cells UR - http://hdl.handle.net/11427/13240 ER - en_ZA
dc.identifier.urihttp://hdl.handle.net/11427/13240
dc.identifier.vancouvercitationPopovic A. Investigating the by-stander effect of Hypericin induced photodynamic therapy on human skin cells. [Thesis]. University of Cape Town ,Faculty of Health Sciences ,Department of Human Biology, 2014 [cited yyyy month dd]. Available from: http://hdl.handle.net/11427/13240en_ZA
dc.language.isoengen_ZA
dc.publisher.departmentDepartment of Human Biologyen_ZA
dc.publisher.facultyFaculty of Health Sciencesen_ZA
dc.publisher.institutionUniversity of Cape Town
dc.subject.otherCell Biologyen_ZA
dc.titleInvestigating the by-stander effect of Hypericin induced photodynamic therapy on human skin cellsen_ZA
dc.typeMaster Thesis
dc.type.qualificationlevelMasters
dc.type.qualificationnameMSc (Med)en_ZA
uct.type.filetypeText
uct.type.filetypeImage
uct.type.publicationResearchen_ZA
uct.type.resourceThesisen_ZA
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