The epidemiology & molecular basis of fluoroquinolone resistant & susceptible isolates of Campylobacter coli

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dc.contributor.advisorElisha, B Gayen_ZA
dc.contributor.advisorLastovica, Alberten_ZA
dc.contributor.authorCooper, Rhetten_ZA
dc.date.accessioned2017-12-11T14:13:56Z
dc.date.available2017-12-11T14:13:56Z
dc.date.issued2001en_ZA
dc.description.abstractFluoroquinolone susceptible and resistant Campylobacter coli were isolated from pigs on two separate pig farms. C. coli are enteric pathogens of humans and animals and although diarrhoea resulting from C. coli and C. jejuni is generally a self-limiting disease, in severe cases, fluoroquinolones are the choice antibiotic for treatment. The presence of fluoroquinolone resistant C. coli strains in the food chain is cause for concern as this may be a source of resistant strains in humans. Sixty-one isolates were included in the study: 26 were susceptible to nalidixic acid and ciprofloxacin and 35 were resistant to these antibiotics. Fifty-five strains were obtained from pigs on farm A, while 6 strains were obtained from pigs on farm B, the source farm of pigs to farm A. Serotyping and flaA typing were carried out to study the epidemiology of the isolates. Serotyping identified 0:24 (11/61) as the most frequent serotype isolated, followed by 0:5 (7/61). Common serotypes 0:48, 0:54 and 0:59 were identified in strains from both farms. A high number of the strains were non-typeable (23/61) but were distinguished by flaA typing. RFLP analysis of the flaA gene revealed 13 distinct profiles in strains from farm A, and 4 profiles in strains from farm B, of which only 1 was unique to farm B. Profile 1 was the commonest profile observed with 31 % (17 /55) of flaA typed strains in this profile. There was an association between 0:24, profile 6, and resistance. Resistant and sensitive pairs were isolated from 15 pigs; flaA profiles of each of 4 pairs were identical, suggesting selection of resistant mutants from previously sensitive populations. An investigation of the molecular basis of the fluoroquinolone resistance identified a Thr-86 to Ile mutation in GyrA, the primary target of these antibiotics.en_ZA
dc.identifier.apacitationCooper, R. (2001). <i>The epidemiology & molecular basis of fluoroquinolone resistant & susceptible isolates of Campylobacter coli</i>. (Thesis). University of Cape Town ,Faculty of Health Sciences ,Division of Medical Microbiology. Retrieved from http://hdl.handle.net/11427/26542en_ZA
dc.identifier.chicagocitationCooper, Rhett. <i>"The epidemiology & molecular basis of fluoroquinolone resistant & susceptible isolates of Campylobacter coli."</i> Thesis., University of Cape Town ,Faculty of Health Sciences ,Division of Medical Microbiology, 2001. http://hdl.handle.net/11427/26542en_ZA
dc.identifier.citationCooper, R. 2001. The epidemiology & molecular basis of fluoroquinolone resistant & susceptible isolates of Campylobacter coli. University of Cape Town.en_ZA
dc.identifier.ris TY - Thesis / Dissertation AU - Cooper, Rhett AB - Fluoroquinolone susceptible and resistant Campylobacter coli were isolated from pigs on two separate pig farms. C. coli are enteric pathogens of humans and animals and although diarrhoea resulting from C. coli and C. jejuni is generally a self-limiting disease, in severe cases, fluoroquinolones are the choice antibiotic for treatment. The presence of fluoroquinolone resistant C. coli strains in the food chain is cause for concern as this may be a source of resistant strains in humans. Sixty-one isolates were included in the study: 26 were susceptible to nalidixic acid and ciprofloxacin and 35 were resistant to these antibiotics. Fifty-five strains were obtained from pigs on farm A, while 6 strains were obtained from pigs on farm B, the source farm of pigs to farm A. Serotyping and flaA typing were carried out to study the epidemiology of the isolates. Serotyping identified 0:24 (11/61) as the most frequent serotype isolated, followed by 0:5 (7/61). Common serotypes 0:48, 0:54 and 0:59 were identified in strains from both farms. A high number of the strains were non-typeable (23/61) but were distinguished by flaA typing. RFLP analysis of the flaA gene revealed 13 distinct profiles in strains from farm A, and 4 profiles in strains from farm B, of which only 1 was unique to farm B. Profile 1 was the commonest profile observed with 31 % (17 /55) of flaA typed strains in this profile. There was an association between 0:24, profile 6, and resistance. Resistant and sensitive pairs were isolated from 15 pigs; flaA profiles of each of 4 pairs were identical, suggesting selection of resistant mutants from previously sensitive populations. An investigation of the molecular basis of the fluoroquinolone resistance identified a Thr-86 to Ile mutation in GyrA, the primary target of these antibiotics. DA - 2001 DB - OpenUCT DP - University of Cape Town LK - https://open.uct.ac.za PB - University of Cape Town PY - 2001 T1 - The epidemiology & molecular basis of fluoroquinolone resistant & susceptible isolates of Campylobacter coli TI - The epidemiology & molecular basis of fluoroquinolone resistant & susceptible isolates of Campylobacter coli UR - http://hdl.handle.net/11427/26542 ER - en_ZA
dc.identifier.urihttp://hdl.handle.net/11427/26542
dc.identifier.vancouvercitationCooper R. The epidemiology & molecular basis of fluoroquinolone resistant & susceptible isolates of Campylobacter coli. [Thesis]. University of Cape Town ,Faculty of Health Sciences ,Division of Medical Microbiology, 2001 [cited yyyy month dd]. Available from: http://hdl.handle.net/11427/26542en_ZA
dc.language.isoengen_ZA
dc.publisher.departmentDivision of Medical Microbiologyen_ZA
dc.publisher.facultyFaculty of Health Sciencesen_ZA
dc.publisher.institutionUniversity of Cape Town
dc.subject.otherMedical Microbiologyen_ZA
dc.titleThe epidemiology & molecular basis of fluoroquinolone resistant & susceptible isolates of Campylobacter colien_ZA
dc.typeMaster Thesis
dc.type.qualificationlevelMasters
dc.type.qualificationnameMSc (Med)en_ZA
uct.type.filetypeText
uct.type.filetypeImage
uct.type.publicationResearchen_ZA
uct.type.resourceThesisen_ZA
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