Viable chimaeric viruses confirm the biological importance of sequence specific maize streak virus movement protein and coat protein interactions

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dc.contributor.authorvan der Walt, Ericen_ZA
dc.contributor.authorPalmer, Kennethen_ZA
dc.contributor.authorMartin, Darrenen_ZA
dc.contributor.authorRybicki, Edwarden_ZA
dc.date.accessioned2015-10-28T07:03:42Z
dc.date.available2015-10-28T07:03:42Z
dc.date.issued2008en_ZA
dc.description.abstractBACKGROUND: A variety of interactions between up to three different movement proteins (MPs), the coat protein (CP) and genomic DNA mediate the inter- and intra-cellular movement of geminiviruses in the genus Begomovirus. Although movement of viruses in the genus Mastrevirus is less well characterized, direct interactions between a single MP and the CP of these viruses is also clearly involved in both intra- and intercellular trafficking of virus genomic DNA. However, it is currently unknown how specific these MP-CP interactions are, nor how disruption of these interactions might impact on virus viability. RESULTS: Using chimaeric genomes of two strains of Maize streak virus (MSV) we adopted a genetic approach to investigate the gross biological effects of interfering with interactions between virus MP and CP homologues derived from genetically distinct MSV isolates. MP and CP genes were reciprocally exchanged, individually and in pairs, between maize (MSV-Kom)- and Setaria sp. (MSV-Set)-adapted isolates sharing 78% genome-wide sequence identity. All chimaeras were infectious in Zea mays c.v. Jubilee and were characterized in terms of symptomatology and infection efficiency. Compared with their parental viruses, all the chimaeras were attenuated in symptom severity, infection efficiency, and the rate at which symptoms appeared. The exchange of individual MP and CP genes resulted in lower infection efficiency and reduced symptom severity in comparison with exchanges of matched MP-CP pairs. CONCLUSION: Specific interactions between the mastrevirus MP and CP genes themselves and/or their expression products are important determinants of infection efficiency, rate of symptom development and symptom severity.en_ZA
dc.identifier.apacitationvan der Walt, E., Palmer, K., Martin, D., & Rybicki, E. (2008). Viable chimaeric viruses confirm the biological importance of sequence specific maize streak virus movement protein and coat protein interactions. <i>Virology Journal</i>, http://hdl.handle.net/11427/14464en_ZA
dc.identifier.chicagocitationvan der Walt, Eric, Kenneth Palmer, Darren Martin, and Edward Rybicki "Viable chimaeric viruses confirm the biological importance of sequence specific maize streak virus movement protein and coat protein interactions." <i>Virology Journal</i> (2008) http://hdl.handle.net/11427/14464en_ZA
dc.identifier.citationvan der Walt, E., Palmer, K. E., Martin, D. P., & Rybicki, E. P. (2008). Viable chimaeric viruses confirm the biological importance of sequence specific maize streak virus movement protein and coat protein interactions. Virol J, 5(11).en_ZA
dc.identifier.ris TY - Journal Article AU - van der Walt, Eric AU - Palmer, Kenneth AU - Martin, Darren AU - Rybicki, Edward AB - BACKGROUND: A variety of interactions between up to three different movement proteins (MPs), the coat protein (CP) and genomic DNA mediate the inter- and intra-cellular movement of geminiviruses in the genus Begomovirus. Although movement of viruses in the genus Mastrevirus is less well characterized, direct interactions between a single MP and the CP of these viruses is also clearly involved in both intra- and intercellular trafficking of virus genomic DNA. However, it is currently unknown how specific these MP-CP interactions are, nor how disruption of these interactions might impact on virus viability. RESULTS: Using chimaeric genomes of two strains of Maize streak virus (MSV) we adopted a genetic approach to investigate the gross biological effects of interfering with interactions between virus MP and CP homologues derived from genetically distinct MSV isolates. MP and CP genes were reciprocally exchanged, individually and in pairs, between maize (MSV-Kom)- and Setaria sp. (MSV-Set)-adapted isolates sharing 78% genome-wide sequence identity. All chimaeras were infectious in Zea mays c.v. Jubilee and were characterized in terms of symptomatology and infection efficiency. Compared with their parental viruses, all the chimaeras were attenuated in symptom severity, infection efficiency, and the rate at which symptoms appeared. The exchange of individual MP and CP genes resulted in lower infection efficiency and reduced symptom severity in comparison with exchanges of matched MP-CP pairs. CONCLUSION: Specific interactions between the mastrevirus MP and CP genes themselves and/or their expression products are important determinants of infection efficiency, rate of symptom development and symptom severity. DA - 2008 DB - OpenUCT DO - 10.1186/1743-422X-5-61 DP - University of Cape Town J1 - Virology Journal LK - https://open.uct.ac.za PB - University of Cape Town PY - 2008 T1 - Viable chimaeric viruses confirm the biological importance of sequence specific maize streak virus movement protein and coat protein interactions TI - Viable chimaeric viruses confirm the biological importance of sequence specific maize streak virus movement protein and coat protein interactions UR - http://hdl.handle.net/11427/14464 ER - en_ZA
dc.identifier.urihttp://hdl.handle.net/11427/14464
dc.identifier.urihttp://dx.doi.org/10.1186/1743-422X-5-61
dc.identifier.vancouvercitationvan der Walt E, Palmer K, Martin D, Rybicki E. Viable chimaeric viruses confirm the biological importance of sequence specific maize streak virus movement protein and coat protein interactions. Virology Journal. 2008; http://hdl.handle.net/11427/14464.en_ZA
dc.language.isoengen_ZA
dc.publisherBioMed Central Ltden_ZA
dc.publisher.departmentDepartment of Molecular and Cell Biologyen_ZA
dc.publisher.facultyFaculty of Scienceen_ZA
dc.publisher.institutionUniversity of Cape Town
dc.rightsThis is an Open Access article distributed under the terms of the Creative Commons Attribution Licenseen_ZA
dc.rights.holder2008 van der Walt et al; licensee BioMed Central Ltd.en_ZA
dc.rights.urihttp://creativecommons.org/licenses/by/2.0en_ZA
dc.sourceVirology Journalen_ZA
dc.source.urihttp://www.virologyj.com/en_ZA
dc.subject.otherMaize streak virusen_ZA
dc.subject.otherPlant Diseasesen_ZA
dc.subject.otherRecombination, Geneticen_ZA
dc.titleViable chimaeric viruses confirm the biological importance of sequence specific maize streak virus movement protein and coat protein interactionsen_ZA
dc.typeJournal Articleen_ZA
uct.type.filetypeText
uct.type.filetypeImage
uct.type.publicationResearchen_ZA
uct.type.resourceArticleen_ZA
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