DNA comparisons of the two orthopoxviruses monkeypox and variola

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dc.contributor.advisorDumbell, K Ren_ZA
dc.contributor.authorPare, Nicola Jenniferen_ZA
dc.date.accessioned2017-12-13T14:16:02Z
dc.date.available2017-12-13T14:16:02Z
dc.date.issued1988en_ZA
dc.description.abstractAlthough smallpox has been eradicated there are animal poxviruses which are closely related. It is desirable to measure the closeness of this relation to assess whether Variola virus could re-emerge as a complex mutant of an animal poxvirus. The most likely candidate is Monkeypox, which can produce human infection clinically resembling smallpox. The work in this thesis is the beginning of a detailed comparison of the DNA of Variola and Monkeypox. A 15.3kb section of the Variola genome was compared with a corresponding 14.4kb region of Monkeypox. This enabled both a comparison of corresponding sequences and the location of a short sequence present only in variola. Initially restriction enzyme mapping of the two stretches of DNA showed considerable homology and narrowed down the area containing any nonhomologous Variola sequences to within 2.9kb. Sequence comparisons show a level of 96% similarity. When the 2.9kb Variola fragment was compared with the corresponding 2.4kb Monkeypox fragment, a 400bp insert was found in Variola flanked by sequences common to both viruses. Analysis of the insert revealed two overlapping open reading frames present on opposite DNA strands. The DNA and putative polypeptide sequences were compared with known sequences, but no significant homology was detected. The presence or absence of this sequence in other orthopoxviruses is being established, but the expression of these open reading frames in vivo and function of the putative polypeptides is still to be investigated.en_ZA
dc.identifier.apacitationPare, N. J. (1988). <i>DNA comparisons of the two orthopoxviruses monkeypox and variola</i>. (Thesis). University of Cape Town ,Faculty of Health Sciences ,Division of Medical Biochemistry & Structural Biology. Retrieved from http://hdl.handle.net/11427/26601en_ZA
dc.identifier.chicagocitationPare, Nicola Jennifer. <i>"DNA comparisons of the two orthopoxviruses monkeypox and variola."</i> Thesis., University of Cape Town ,Faculty of Health Sciences ,Division of Medical Biochemistry & Structural Biology, 1988. http://hdl.handle.net/11427/26601en_ZA
dc.identifier.citationPare, N. 1988. DNA comparisons of the two orthopoxviruses monkeypox and variola. University of Cape Town.en_ZA
dc.identifier.ris TY - Thesis / Dissertation AU - Pare, Nicola Jennifer AB - Although smallpox has been eradicated there are animal poxviruses which are closely related. It is desirable to measure the closeness of this relation to assess whether Variola virus could re-emerge as a complex mutant of an animal poxvirus. The most likely candidate is Monkeypox, which can produce human infection clinically resembling smallpox. The work in this thesis is the beginning of a detailed comparison of the DNA of Variola and Monkeypox. A 15.3kb section of the Variola genome was compared with a corresponding 14.4kb region of Monkeypox. This enabled both a comparison of corresponding sequences and the location of a short sequence present only in variola. Initially restriction enzyme mapping of the two stretches of DNA showed considerable homology and narrowed down the area containing any nonhomologous Variola sequences to within 2.9kb. Sequence comparisons show a level of 96% similarity. When the 2.9kb Variola fragment was compared with the corresponding 2.4kb Monkeypox fragment, a 400bp insert was found in Variola flanked by sequences common to both viruses. Analysis of the insert revealed two overlapping open reading frames present on opposite DNA strands. The DNA and putative polypeptide sequences were compared with known sequences, but no significant homology was detected. The presence or absence of this sequence in other orthopoxviruses is being established, but the expression of these open reading frames in vivo and function of the putative polypeptides is still to be investigated. DA - 1988 DB - OpenUCT DP - University of Cape Town LK - https://open.uct.ac.za PB - University of Cape Town PY - 1988 T1 - DNA comparisons of the two orthopoxviruses monkeypox and variola TI - DNA comparisons of the two orthopoxviruses monkeypox and variola UR - http://hdl.handle.net/11427/26601 ER - en_ZA
dc.identifier.urihttp://hdl.handle.net/11427/26601
dc.identifier.vancouvercitationPare NJ. DNA comparisons of the two orthopoxviruses monkeypox and variola. [Thesis]. University of Cape Town ,Faculty of Health Sciences ,Division of Medical Biochemistry & Structural Biology, 1988 [cited yyyy month dd]. Available from: http://hdl.handle.net/11427/26601en_ZA
dc.language.isoengen_ZA
dc.publisher.departmentDivision of Medical Biochemistry and Structural Biology
dc.publisher.facultyFaculty of Health Sciencesen_ZA
dc.publisher.institutionUniversity of Cape Town
dc.subject.otherPoxvirusesen_ZA
dc.titleDNA comparisons of the two orthopoxviruses monkeypox and variolaen_ZA
dc.typeMaster Thesis
dc.type.qualificationlevelMasters
dc.type.qualificationnameMSc (Med)en_ZA
uct.type.filetypeText
uct.type.filetypeImage
uct.type.publicationResearchen_ZA
uct.type.resourceThesisen_ZA
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