Phylogeographic structure in the CFR genus Pauridia revealed by inter-simple sequence repeat amplification

dc.contributor.advisorHedderson, Terry Aen_ZA
dc.contributor.authorBurman, Marcen_ZA
dc.date.accessioned2017-11-13T08:19:52Z
dc.date.available2017-11-13T08:19:52Z
dc.date.issued2005en_ZA
dc.date.updated2017-02-02T13:39:57Z
dc.description.abstractThe Cape Floristic Region, South Africa, has high numbers of rare and endemic plants. Many, including Pauridia Harv., are geophytes in the Hypoxidaceae. The two species of Pauridia differ in morphology and range, with P. minuta occupying a wide range across lowland CFR and P. longituba being restricted to granite outcrops on the West coast. Genetic structure correlated to geography has been described for the haploid chloroplast genome. Here the diploid nuclear genome is investigated using inter-simple sequence repeat amplification. Eleven populations were sampled from DNA collected for a previous study. Analysis of molecular variance was done. Ordination based on similarity and covariance was done to detect structure. Most of the variance was found to be within populations (65.54%) and between populations within groups (35.69%). Some variance (10.10%) was found between P. minuta geographic groups. Principal Component Analysis revealed a little structure, grouping some similar haplotypes together. Cluster analysis placed P. longituba within P. minuta, reflecting the haplotypic structure. Differences in structure revealed between the chloroplast and nuclear genome may be explained by differences in gene flow resulting from the different modes of inheritance - chloroplast via seed and nuclear via pollen and seed. Further analysis of genetic distance correlations between the chloroplast and nuclear data would provide a useful quantitative measure of difference in structure.en_ZA
dc.identifier.apacitationBurman, M. (2005). <i>Phylogeographic structure in the CFR genus Pauridia revealed by inter-simple sequence repeat amplification</i>. (Thesis). University of Cape Town ,Faculty of Science ,Department of Biological Sciences. Retrieved from http://hdl.handle.net/11427/26185en_ZA
dc.identifier.chicagocitationBurman, Marc. <i>"Phylogeographic structure in the CFR genus Pauridia revealed by inter-simple sequence repeat amplification."</i> Thesis., University of Cape Town ,Faculty of Science ,Department of Biological Sciences, 2005. http://hdl.handle.net/11427/26185en_ZA
dc.identifier.citationBurman, M. 2005. Phylogeographic structure in the CFR genus Pauridia revealed by inter-simple sequence repeat amplification. University of Cape Town.en_ZA
dc.identifier.ris TY - Thesis / Dissertation AU - Burman, Marc AB - The Cape Floristic Region, South Africa, has high numbers of rare and endemic plants. Many, including Pauridia Harv., are geophytes in the Hypoxidaceae. The two species of Pauridia differ in morphology and range, with P. minuta occupying a wide range across lowland CFR and P. longituba being restricted to granite outcrops on the West coast. Genetic structure correlated to geography has been described for the haploid chloroplast genome. Here the diploid nuclear genome is investigated using inter-simple sequence repeat amplification. Eleven populations were sampled from DNA collected for a previous study. Analysis of molecular variance was done. Ordination based on similarity and covariance was done to detect structure. Most of the variance was found to be within populations (65.54%) and between populations within groups (35.69%). Some variance (10.10%) was found between P. minuta geographic groups. Principal Component Analysis revealed a little structure, grouping some similar haplotypes together. Cluster analysis placed P. longituba within P. minuta, reflecting the haplotypic structure. Differences in structure revealed between the chloroplast and nuclear genome may be explained by differences in gene flow resulting from the different modes of inheritance - chloroplast via seed and nuclear via pollen and seed. Further analysis of genetic distance correlations between the chloroplast and nuclear data would provide a useful quantitative measure of difference in structure. DA - 2005 DB - OpenUCT DP - University of Cape Town LK - https://open.uct.ac.za PB - University of Cape Town PY - 2005 T1 - Phylogeographic structure in the CFR genus Pauridia revealed by inter-simple sequence repeat amplification TI - Phylogeographic structure in the CFR genus Pauridia revealed by inter-simple sequence repeat amplification UR - http://hdl.handle.net/11427/26185 ER - en_ZA
dc.identifier.urihttp://hdl.handle.net/11427/26185
dc.identifier.vancouvercitationBurman M. Phylogeographic structure in the CFR genus Pauridia revealed by inter-simple sequence repeat amplification. [Thesis]. University of Cape Town ,Faculty of Science ,Department of Biological Sciences, 2005 [cited yyyy month dd]. Available from: http://hdl.handle.net/11427/26185en_ZA
dc.language.isoengen_ZA
dc.publisher.departmentDepartment of Biological Sciencesen_ZA
dc.publisher.facultyFaculty of Scienceen_ZA
dc.publisher.institutionUniversity of Cape Town
dc.subject.otherBotanyen_ZA
dc.subject.otherSystematicsen_ZA
dc.titlePhylogeographic structure in the CFR genus Pauridia revealed by inter-simple sequence repeat amplificationen_ZA
dc.typeBachelor Thesis
dc.type.qualificationlevelHonours
dc.type.qualificationnameBSc (Hons)en_ZA
uct.type.filetype
uct.type.filetypeText
uct.type.filetypeImage
uct.type.publicationResearchen_ZA
uct.type.resourceThesisen_ZA
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