CD8+ T-cell interleukin-7 receptor alpha expression as a potential indicator of disease status in HIV-infected children

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dc.contributor.authorSharma, Tanvi Sen_ZA
dc.contributor.authorHughes, Janeen_ZA
dc.contributor.authorMurillo, Amarylisen_ZA
dc.contributor.authorRiley, Joanneen_ZA
dc.contributor.authorSoares, Andreiaen_ZA
dc.contributor.authorLittle, Francescaen_ZA
dc.contributor.authorMitchell, Charles Den_ZA
dc.contributor.authorHanekom, Willem Aen_ZA
dc.date.accessioned2015-11-16T04:12:51Z
dc.date.available2015-11-16T04:12:51Z
dc.date.issued2008en_ZA
dc.description.abstractBACKGROUND: Initiation and modification of antiretroviral therapy in HIV-infected children depend on viral load and CD4+ T-cell count. However, these surrogates have limitations, and complementary immunological markers to assess therapeutic response are needed. Our aim was to evaluate CD8+ T-cell expression of CD127 as a marker of disease status in HIV-infected children, based on adult data suggesting its usefulness. We hypothesized that CD127 expression on CD8+ T-cells is lower in children with more advanced disease. METHODS: In a cross-sectional evaluation, we used flow cytometry to measure CD127+ expression on CD8+ T-cells in whole blood from HIV-infected children with varying disease status. This was compared with expression of CD38 on this subset, currently used in clinical practice as a marker of disease status. RESULTS: 51 HIV-infected children were enrolled. There was a strong positive correlation between CD127 expression on CD8+ T-cells and CD4+ T-cell count, and height and weight z-scores, and a strong negative correlation between CD127 expression and viral load. In contrast, we found no association between CD38 expression and these disease status markers. CONCLUSIONS: CD8+ T-cell CD127 expression is significantly higher in children with better HIV disease control, and may have a role as an immunologic indicator of disease status. Longitudinal studies are needed to determine the utility of this marker as a potential indicator of HIV disease progression.en_ZA
dc.identifier.apacitationSharma, T. S., Hughes, J., Murillo, A., Riley, J., Soares, A., Little, F., ... Hanekom, W. A. (2008). CD8+ T-cell interleukin-7 receptor alpha expression as a potential indicator of disease status in HIV-infected children. <i>PLoS One</i>, http://hdl.handle.net/11427/15026en_ZA
dc.identifier.chicagocitationSharma, Tanvi S, Jane Hughes, Amarylis Murillo, Joanne Riley, Andreia Soares, Francesca Little, Charles D Mitchell, and Willem A Hanekom "CD8+ T-cell interleukin-7 receptor alpha expression as a potential indicator of disease status in HIV-infected children." <i>PLoS One</i> (2008) http://hdl.handle.net/11427/15026en_ZA
dc.identifier.citationSharma, T. S., Hughes, J., Murillo, A., Riley, J., Soares, A., Little, F., ... & Hanekom, W. A. (2008). CD8+ T-cell interleukin-7 receptor alpha expression as a potential indicator of disease status in HIV-infected children. PloS one, 3(12), e3986. doi:10.1371/journal.pone.0003986en_ZA
dc.identifier.ris TY - Journal Article AU - Sharma, Tanvi S AU - Hughes, Jane AU - Murillo, Amarylis AU - Riley, Joanne AU - Soares, Andreia AU - Little, Francesca AU - Mitchell, Charles D AU - Hanekom, Willem A AB - BACKGROUND: Initiation and modification of antiretroviral therapy in HIV-infected children depend on viral load and CD4+ T-cell count. However, these surrogates have limitations, and complementary immunological markers to assess therapeutic response are needed. Our aim was to evaluate CD8+ T-cell expression of CD127 as a marker of disease status in HIV-infected children, based on adult data suggesting its usefulness. We hypothesized that CD127 expression on CD8+ T-cells is lower in children with more advanced disease. METHODS: In a cross-sectional evaluation, we used flow cytometry to measure CD127+ expression on CD8+ T-cells in whole blood from HIV-infected children with varying disease status. This was compared with expression of CD38 on this subset, currently used in clinical practice as a marker of disease status. RESULTS: 51 HIV-infected children were enrolled. There was a strong positive correlation between CD127 expression on CD8+ T-cells and CD4+ T-cell count, and height and weight z-scores, and a strong negative correlation between CD127 expression and viral load. In contrast, we found no association between CD38 expression and these disease status markers. CONCLUSIONS: CD8+ T-cell CD127 expression is significantly higher in children with better HIV disease control, and may have a role as an immunologic indicator of disease status. Longitudinal studies are needed to determine the utility of this marker as a potential indicator of HIV disease progression. DA - 2008 DB - OpenUCT DO - 10.1371/journal.pone.0003986 DP - University of Cape Town J1 - PLoS One LK - https://open.uct.ac.za PB - University of Cape Town PY - 2008 T1 - CD8+ T-cell interleukin-7 receptor alpha expression as a potential indicator of disease status in HIV-infected children TI - CD8+ T-cell interleukin-7 receptor alpha expression as a potential indicator of disease status in HIV-infected children UR - http://hdl.handle.net/11427/15026 ER - en_ZA
dc.identifier.urihttp://hdl.handle.net/11427/15026
dc.identifier.urihttp://dx.doi.org/10.1371/journal.pone.0003986
dc.identifier.vancouvercitationSharma TS, Hughes J, Murillo A, Riley J, Soares A, Little F, et al. CD8+ T-cell interleukin-7 receptor alpha expression as a potential indicator of disease status in HIV-infected children. PLoS One. 2008; http://hdl.handle.net/11427/15026.en_ZA
dc.language.isoengen_ZA
dc.publisherPublic Library of Scienceen_ZA
dc.publisher.departmentDepartment of Statistical Sciencesen_ZA
dc.publisher.facultyFaculty of Scienceen_ZA
dc.publisher.institutionUniversity of Cape Town
dc.rightsThis is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.en_ZA
dc.rights.holder© 2008 Sharma et alen_ZA
dc.rights.urihttp://creativecommons.org/licenses/by/4.0en_ZA
dc.sourcePLoS Oneen_ZA
dc.source.urihttp://journals.plos.org/plosoneen_ZA
dc.subject.otherT cellsen_ZA
dc.subject.otherCytotoxic T cellsen_ZA
dc.subject.otherHIVen_ZA
dc.subject.otherViral loaden_ZA
dc.subject.otherLymphocytesen_ZA
dc.subject.otherAntiretroviral therapyen_ZA
dc.subject.otherChildrenen_ZA
dc.subject.otherHIV clinical manifestationsen_ZA
dc.titleCD8+ T-cell interleukin-7 receptor alpha expression as a potential indicator of disease status in HIV-infected childrenen_ZA
dc.typeJournal Articleen_ZA
uct.type.filetypeText
uct.type.filetypeImage
uct.type.publicationResearchen_ZA
uct.type.resourceArticleen_ZA
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