High yield of culture-based diagnosis in a TB-endemic setting
dc.contributor.author | Demers, Anne-Marie | en_ZA |
dc.contributor.author | Verver, Suzanne | en_ZA |
dc.contributor.author | Boulle, Andrew | en_ZA |
dc.contributor.author | Warren, Robin | en_ZA |
dc.contributor.author | van Helden, Paul | en_ZA |
dc.contributor.author | Behr, Marcel | en_ZA |
dc.contributor.author | Coetzee, David | en_ZA |
dc.date.accessioned | 2015-11-18T03:58:42Z | |
dc.date.available | 2015-11-18T03:58:42Z | |
dc.date.issued | 2012 | en_ZA |
dc.description.abstract | BACKGROUND: In most of the world, microbiologic diagnosis of tuberculosis (TB) is limited to microscopy. Recent guidelines recommend culture-based diagnosis where feasible. METHODS: In order to evaluate the relative and absolute incremental diagnostic yield of culture-based diagnosis in a high-incidence community in Cape Town, South Africa, subjects evaluated for suspected TB had their samples processed for microscopy and culture over a 21 month period. RESULTS: For 2537 suspect episodes with 2 smears and 2 cultures done, 20.0% (508) had at least one positive smear and 29.9% (760) had at least one positive culture. One culture yielded 1.8 times more cases as 1 smear (relative yield), or an increase of 12.0% (absolute yield). Based on the latter value, the number of cultures needed to diagnose (NND) one extra case of TB was 8, compared to 19 if second specimens were submitted for microscopy. CONCLUSION: In a high-burden setting, the introduction of culture can markedly increase TB diagnosis over microscopy. The concept of number needed to diagnose can help in comparing incremental yield of diagnosis methods. Although new promising diagnostic molecular methods are being implemented, TB culture is still the gold standard. | en_ZA |
dc.identifier.apacitation | Demers, A., Verver, S., Boulle, A., Warren, R., van Helden, P., Behr, M., & Coetzee, D. (2012). High yield of culture-based diagnosis in a TB-endemic setting. <i>BMC Infectious Diseases</i>, http://hdl.handle.net/11427/15077 | en_ZA |
dc.identifier.chicagocitation | Demers, Anne-Marie, Suzanne Verver, Andrew Boulle, Robin Warren, Paul van Helden, Marcel Behr, and David Coetzee "High yield of culture-based diagnosis in a TB-endemic setting." <i>BMC Infectious Diseases</i> (2012) http://hdl.handle.net/11427/15077 | en_ZA |
dc.identifier.citation | Demers, A. M., Verver, S., Boulle, A., Warren, R., Van Helden, P., Behr, M. A., & Coetzee, D. (2012). High yield of culture-based diagnosis in a TB-endemic setting. BMC infectious diseases, 12(1), 218. | en_ZA |
dc.identifier.ris | TY - Journal Article AU - Demers, Anne-Marie AU - Verver, Suzanne AU - Boulle, Andrew AU - Warren, Robin AU - van Helden, Paul AU - Behr, Marcel AU - Coetzee, David AB - BACKGROUND: In most of the world, microbiologic diagnosis of tuberculosis (TB) is limited to microscopy. Recent guidelines recommend culture-based diagnosis where feasible. METHODS: In order to evaluate the relative and absolute incremental diagnostic yield of culture-based diagnosis in a high-incidence community in Cape Town, South Africa, subjects evaluated for suspected TB had their samples processed for microscopy and culture over a 21 month period. RESULTS: For 2537 suspect episodes with 2 smears and 2 cultures done, 20.0% (508) had at least one positive smear and 29.9% (760) had at least one positive culture. One culture yielded 1.8 times more cases as 1 smear (relative yield), or an increase of 12.0% (absolute yield). Based on the latter value, the number of cultures needed to diagnose (NND) one extra case of TB was 8, compared to 19 if second specimens were submitted for microscopy. CONCLUSION: In a high-burden setting, the introduction of culture can markedly increase TB diagnosis over microscopy. The concept of number needed to diagnose can help in comparing incremental yield of diagnosis methods. Although new promising diagnostic molecular methods are being implemented, TB culture is still the gold standard. DA - 2012 DB - OpenUCT DO - 10.1186/1471-2334-12-218 DP - University of Cape Town J1 - BMC Infectious Diseases LK - https://open.uct.ac.za PB - University of Cape Town PY - 2012 T1 - High yield of culture-based diagnosis in a TB-endemic setting TI - High yield of culture-based diagnosis in a TB-endemic setting UR - http://hdl.handle.net/11427/15077 ER - | en_ZA |
dc.identifier.uri | http://hdl.handle.net/11427/15077 | |
dc.identifier.uri | http://dx.doi.org/10.1186/1471-2334-12-218 | |
dc.identifier.vancouvercitation | Demers A, Verver S, Boulle A, Warren R, van Helden P, Behr M, et al. High yield of culture-based diagnosis in a TB-endemic setting. BMC Infectious Diseases. 2012; http://hdl.handle.net/11427/15077. | en_ZA |
dc.language.iso | eng | en_ZA |
dc.publisher | BioMed Central Ltd | en_ZA |
dc.publisher.department | Institute of Infectious Disease and Molecular Medicine | en_ZA |
dc.publisher.faculty | Faculty of Health Sciences | en_ZA |
dc.publisher.institution | University of Cape Town | |
dc.rights | This is an Open Access article distributed under the terms of the Creative Commons Attribution License | en_ZA |
dc.rights.holder | 2012 Demers et al.; licensee BioMed Central Ltd. | en_ZA |
dc.rights.uri | http://creativecommons.org/licenses/by/2.0 | en_ZA |
dc.source | BMC Infectious Diseases | en_ZA |
dc.source.uri | http://www.biomedcentral.com/bmcinfectdis/ | en_ZA |
dc.subject.other | microbiologic diagnosis | en_ZA |
dc.subject.other | tuberculosis (TB) | en_ZA |
dc.subject.other | microscopy | en_ZA |
dc.title | High yield of culture-based diagnosis in a TB-endemic setting | en_ZA |
dc.type | Journal Article | en_ZA |
uct.type.filetype | Text | |
uct.type.filetype | Image | |
uct.type.publication | Research | en_ZA |
uct.type.resource | Article | en_ZA |
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