Paraoxonase1 Genetic Polymorphisms in a Mixed Ancestry African Population

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dc.contributor.authorMacharia, M
dc.contributor.authorKengne, A P
dc.contributor.authorBlackhurst, D M
dc.contributor.authorErasmus, R T
dc.contributor.authorMatsha, T E
dc.date.accessioned2021-10-08T07:08:21Z
dc.date.available2021-10-08T07:08:21Z
dc.date.issued2014
dc.description.abstractParaoxonase 1 (PON1) activity is markedly influenced by coding polymorphisms, Q/R at position 192 and M/L at position 55 of the PON1 gene. We investigated the frequencies of these polymorphisms and their effects on PON1 and antioxidant activities in 844 South African mixed ancestry individuals. Genotyping was done using allele-specific TaqMan technology, PON1 activities were measured using paraoxon and phenylacetate, oxidative status was determined by measuring the antioxidant activities of ferric reducing antioxidant power and trolox equivalent antioxidant capacity, and lipid peroxidation markers included malondialdehyde and oxidized LDL. The frequencies of Q192R and L55M were 47.6% and 28.8%, respectively, and the most common corresponding alleles were 192R (60.4%) and 55M (82.6%). The Q192 was significantly associated with 5.8 units’ increase in PON1 concentration and 15.4 units’ decrease in PONase activity after adjustment for age, sex, BMI, and diabetes, with suggestion of differential effects by diabetes status. The PON1 L55 variant was associated with none of the measured indices. In conclusion, we have shown that the Q192R polymorphism is a determinant of both PON1 concentration and activity and this association appeared to be enhanced in subjects with diabetes.
dc.identifier.apacitationMacharia, M., Kengne, A. P., Blackhurst, D. M., Erasmus, R. T., & Matsha, T. E. (2014). Paraoxonase1 Genetic Polymorphisms in a Mixed Ancestry African Population. <i>Mediators of Inflammation</i>, 2014(4), 174 - 177. http://hdl.handle.net/11427/34563en_ZA
dc.identifier.chicagocitationMacharia, M, A P Kengne, D M Blackhurst, R T Erasmus, and T E Matsha "Paraoxonase1 Genetic Polymorphisms in a Mixed Ancestry African Population." <i>Mediators of Inflammation</i> 2014, 4. (2014): 174 - 177. http://hdl.handle.net/11427/34563en_ZA
dc.identifier.citationMacharia, M., Kengne, A.P., Blackhurst, D.M., Erasmus, R.T. & Matsha, T.E. 2014. Paraoxonase1 Genetic Polymorphisms in a Mixed Ancestry African Population. <i>Mediators of Inflammation.</i> 2014(4):174 - 177. http://hdl.handle.net/11427/34563en_ZA
dc.identifier.issn0962-9351
dc.identifier.issn1466-1861
dc.identifier.ris TY - Journal Article AU - Macharia, M AU - Kengne, A P AU - Blackhurst, D M AU - Erasmus, R T AU - Matsha, T E AB - Paraoxonase 1 (PON1) activity is markedly influenced by coding polymorphisms, Q/R at position 192 and M/L at position 55 of the PON1 gene. We investigated the frequencies of these polymorphisms and their effects on PON1 and antioxidant activities in 844 South African mixed ancestry individuals. Genotyping was done using allele-specific TaqMan technology, PON1 activities were measured using paraoxon and phenylacetate, oxidative status was determined by measuring the antioxidant activities of ferric reducing antioxidant power and trolox equivalent antioxidant capacity, and lipid peroxidation markers included malondialdehyde and oxidized LDL. The frequencies of Q192R and L55M were 47.6% and 28.8%, respectively, and the most common corresponding alleles were 192R (60.4%) and 55M (82.6%). The Q192 was significantly associated with 5.8 units’ increase in PON1 concentration and 15.4 units’ decrease in PONase activity after adjustment for age, sex, BMI, and diabetes, with suggestion of differential effects by diabetes status. The PON1 L55 variant was associated with none of the measured indices. In conclusion, we have shown that the Q192R polymorphism is a determinant of both PON1 concentration and activity and this association appeared to be enhanced in subjects with diabetes. DA - 2014 DB - OpenUCT DP - University of Cape Town IS - 4 J1 - Mediators of Inflammation LK - https://open.uct.ac.za PY - 2014 SM - 0962-9351 SM - 1466-1861 T1 - Paraoxonase1 Genetic Polymorphisms in a Mixed Ancestry African Population TI - Paraoxonase1 Genetic Polymorphisms in a Mixed Ancestry African Population UR - http://hdl.handle.net/11427/34563 ER - en_ZA
dc.identifier.urihttp://hdl.handle.net/11427/34563
dc.identifier.vancouvercitationMacharia M, Kengne AP, Blackhurst DM, Erasmus RT, Matsha TE. Paraoxonase1 Genetic Polymorphisms in a Mixed Ancestry African Population. Mediators of Inflammation. 2014;2014(4):174 - 177. http://hdl.handle.net/11427/34563.en_ZA
dc.language.isoeng
dc.publisher.departmentDivision of Chemical Pathology
dc.publisher.facultyFaculty of Health Sciences
dc.sourceMediators of Inflammation
dc.source.journalissue4
dc.source.journalvolume2014
dc.source.pagination174 - 177
dc.source.urihttps://dx.doi.org/10.1155/2014/217019
dc.subject.otherAdult
dc.subject.otherAged
dc.subject.otherAryldialkylphosphatase
dc.subject.otherDiabetes Mellitus
dc.subject.otherFemale
dc.subject.otherGene Frequency
dc.subject.otherHumans
dc.subject.otherMale
dc.subject.otherMiddle Aged
dc.subject.otherOxidative Stress
dc.subject.otherPolymorphism, Genetic
dc.subject.otherSouth Africa
dc.subject.otherAryldialkylphosphatase
dc.subject.otherPON1 protein, human
dc.titleParaoxonase1 Genetic Polymorphisms in a Mixed Ancestry African Population
dc.typeJournal Article
uct.type.publicationResearch
uct.type.resourceJournal Article
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