Metabolomics of desiccation tolerance in Xerophyta humilis

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dc.contributor.advisorFarrant, Jill Men_ZA
dc.contributor.advisorRafudeen, M Sen_ZA
dc.contributor.authorDace, Halforden_ZA
dc.date.accessioned2014-11-05T03:46:58Z
dc.date.available2014-11-05T03:46:58Z
dc.date.issued2014en_ZA
dc.descriptionIncludes bibliographical referencesen_ZA
dc.description.abstractResurrection plants are unique in the ability to survive near complete water loss in vegetative tissues without loss of viability. In order to do so, they employ multifaceted strategies which include structural adaptations, antioxidant and photoprotective mechanisms, and the accumulation of proteins and metabolites that stabilise macromolecules. A full understanding of the phenomenon of vegetative desiccation tolerance will require a systems view of these adaptations at the levels of the genome, the control of gene expression, and the control of metabolic pathways. This dissertation reports a high-throughput metabolomic analysis of the changes that occur in vegetative tissues of resurrection plant Xerophyta humilis during dehydration. A combination of chromatography, mass spectrometry and nuclear magnetic resonance revealed numerous primary and secondary metabolites in the plant. Multivariate statistics identified a subset of metabolites that were significantly up- or down-regulated in response to water deficit stress. These metabolites both confirmed existing observations about the metabolic response of X. humilis to drying and revealed compounds not previously known to be associated with this response. Desiccation-associated metabolites were mapped onto known biochemical pathways, to generate hypotheses concerning possible regulatory schemes in the stress response, inviting deeper investigation in future.en_ZA
dc.identifier.apacitationDace, H. (2014). <i>Metabolomics of desiccation tolerance in Xerophyta humilis</i>. (Thesis). University of Cape Town ,Faculty of Science ,Department of Molecular and Cell Biology. Retrieved from http://hdl.handle.net/11427/9111en_ZA
dc.identifier.chicagocitationDace, Halford. <i>"Metabolomics of desiccation tolerance in Xerophyta humilis."</i> Thesis., University of Cape Town ,Faculty of Science ,Department of Molecular and Cell Biology, 2014. http://hdl.handle.net/11427/9111en_ZA
dc.identifier.citationDace, H. 2014. Metabolomics of desiccation tolerance in Xerophyta humilis. University of Cape Town.en_ZA
dc.identifier.ris TY - Thesis / Dissertation AU - Dace, Halford AB - Resurrection plants are unique in the ability to survive near complete water loss in vegetative tissues without loss of viability. In order to do so, they employ multifaceted strategies which include structural adaptations, antioxidant and photoprotective mechanisms, and the accumulation of proteins and metabolites that stabilise macromolecules. A full understanding of the phenomenon of vegetative desiccation tolerance will require a systems view of these adaptations at the levels of the genome, the control of gene expression, and the control of metabolic pathways. This dissertation reports a high-throughput metabolomic analysis of the changes that occur in vegetative tissues of resurrection plant Xerophyta humilis during dehydration. A combination of chromatography, mass spectrometry and nuclear magnetic resonance revealed numerous primary and secondary metabolites in the plant. Multivariate statistics identified a subset of metabolites that were significantly up- or down-regulated in response to water deficit stress. These metabolites both confirmed existing observations about the metabolic response of X. humilis to drying and revealed compounds not previously known to be associated with this response. Desiccation-associated metabolites were mapped onto known biochemical pathways, to generate hypotheses concerning possible regulatory schemes in the stress response, inviting deeper investigation in future. DA - 2014 DB - OpenUCT DP - University of Cape Town LK - https://open.uct.ac.za PB - University of Cape Town PY - 2014 T1 - Metabolomics of desiccation tolerance in Xerophyta humilis TI - Metabolomics of desiccation tolerance in Xerophyta humilis UR - http://hdl.handle.net/11427/9111 ER - en_ZA
dc.identifier.urihttp://hdl.handle.net/11427/9111
dc.identifier.vancouvercitationDace H. Metabolomics of desiccation tolerance in Xerophyta humilis. [Thesis]. University of Cape Town ,Faculty of Science ,Department of Molecular and Cell Biology, 2014 [cited yyyy month dd]. Available from: http://hdl.handle.net/11427/9111en_ZA
dc.language.isoengen_ZA
dc.publisher.departmentDepartment of Molecular and Cell Biologyen_ZA
dc.publisher.facultyFaculty of Scienceen_ZA
dc.publisher.institutionUniversity of Cape Town
dc.titleMetabolomics of desiccation tolerance in Xerophyta humilisen_ZA
dc.typeMaster Thesis
dc.type.qualificationlevelMasters
dc.type.qualificationnameMScen_ZA
uct.type.filetypeText
uct.type.filetypeImage
uct.type.publicationResearchen_ZA
uct.type.resourceThesisen_ZA
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