Replicative intermediates of maize streak virus found during leaf development

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dc.contributor.authorErdmann, Julia B
dc.contributor.authorShepherd, Dionne N
dc.contributor.authorMartin, Darren P
dc.contributor.authorVarsani, Arvind
dc.contributor.authorRybicki, Edward P
dc.contributor.authorJeske, Holger
dc.date.accessioned2016-07-26T07:30:28Z
dc.date.available2016-07-26T07:30:28Z
dc.date.issued2010
dc.date.updated2016-07-15T16:12:39Z
dc.description.abstractGeminiviruses of the genera Begomovirus and Curtovirus utilize three replication modes: complementary-strand replication (CSR), rolling-circle replication (RCR) and recombinationdependent replication (RDR). Using two-dimensional gel electrophoresis, we now show for the first time that maize streak virus (MSV), the type member of the most divergent geminivirus genus, Mastrevirus, does the same. Although mastreviruses have fewer regulatory genes than other geminiviruses and uniquely express their replication-associated protein (Rep) from a spliced transcript, the replicative intermediates of CSR, RCR and RDR could be detected unequivocally within infected maize tissues. All replicative intermediates accumulated early and, to varying degrees, were already present in the shoot apex and leaves at different maturation stages. Relative to other replicative intermediates, those associated with RCR increased in prevalence during leaf maturation. Interestingly, in addition to RCR-associated DNA forms seen in other geminiviruses, MSV also apparently uses dimeric open circular DNA as a template for RCR.en_ZA
dc.identifierhttp://dx.doi.org/10.1099/vir.0.017574-0
dc.identifier.apacitationErdmann, J. B., Shepherd, D. N., Martin, D. P., Varsani, A., Rybicki, E. P., & Jeske, H. (2010). Replicative intermediates of maize streak virus found during leaf development. <i>Journal of General Virology</i>, http://hdl.handle.net/11427/20735en_ZA
dc.identifier.chicagocitationErdmann, Julia B, Dionne N Shepherd, Darren P Martin, Arvind Varsani, Edward P Rybicki, and Holger Jeske "Replicative intermediates of maize streak virus found during leaf development." <i>Journal of General Virology</i> (2010) http://hdl.handle.net/11427/20735en_ZA
dc.identifier.citationErdmann, J. B., Shepherd, D. N., Martin, D. P., Varsani, A., Rybicki, E. P., & Jeske, H. (2010). Replicative intermediates of maize streak virus found during leaf development. Journal of general virology, 91(4), 1077-1081.en_ZA
dc.identifier.issn0022-1317en_ZA
dc.identifier.ris TY - Journal Article AU - Erdmann, Julia B AU - Shepherd, Dionne N AU - Martin, Darren P AU - Varsani, Arvind AU - Rybicki, Edward P AU - Jeske, Holger AB - Geminiviruses of the genera Begomovirus and Curtovirus utilize three replication modes: complementary-strand replication (CSR), rolling-circle replication (RCR) and recombinationdependent replication (RDR). Using two-dimensional gel electrophoresis, we now show for the first time that maize streak virus (MSV), the type member of the most divergent geminivirus genus, Mastrevirus, does the same. Although mastreviruses have fewer regulatory genes than other geminiviruses and uniquely express their replication-associated protein (Rep) from a spliced transcript, the replicative intermediates of CSR, RCR and RDR could be detected unequivocally within infected maize tissues. All replicative intermediates accumulated early and, to varying degrees, were already present in the shoot apex and leaves at different maturation stages. Relative to other replicative intermediates, those associated with RCR increased in prevalence during leaf maturation. Interestingly, in addition to RCR-associated DNA forms seen in other geminiviruses, MSV also apparently uses dimeric open circular DNA as a template for RCR. DA - 2010 DB - OpenUCT DP - University of Cape Town J1 - Journal of General Virology LK - https://open.uct.ac.za PB - University of Cape Town PY - 2010 SM - 0022-1317 T1 - Replicative intermediates of maize streak virus found during leaf development TI - Replicative intermediates of maize streak virus found during leaf development UR - http://hdl.handle.net/11427/20735 ER - en_ZA
dc.identifier.urihttp://hdl.handle.net/11427/20735
dc.identifier.vancouvercitationErdmann JB, Shepherd DN, Martin DP, Varsani A, Rybicki EP, Jeske H. Replicative intermediates of maize streak virus found during leaf development. Journal of General Virology. 2010; http://hdl.handle.net/11427/20735.en_ZA
dc.languageengen_ZA
dc.publisherMicrobiology Societyen_ZA
dc.publisher.institutionUniversity of Cape Town
dc.sourceJournal of General Virologyen_ZA
dc.source.urihttp://jgv.microbiologyresearch.org/content/journal/jgv
dc.titleReplicative intermediates of maize streak virus found during leaf developmenten_ZA
dc.typeJournal Articleen_ZA
uct.type.filetypeText
uct.type.filetypeImage
uct.type.publicationResearchen_ZA
uct.type.resourceArticleen_ZA
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