Competition between the purine and pyrimidine triple helix motifs in an oligonucleotide system

dc.contributor.advisorKlump, Horsten_ZA
dc.contributor.authorMills, Martin Georgeen_ZA
dc.date.accessioned2016-03-17T12:41:06Z
dc.date.available2016-03-17T12:41:06Z
dc.date.issued1998en_ZA
dc.descriptionBibliography: p. 86-109.en_ZA
dc.description.abstractTriple helices are classified into two groups according to the composition and orientation of the third strand, namely the pyrimidine motif and the purine motif. These motifs constitute two separate fields of research. It was proposed, based on the alternative design rules, that the two motifs can in fact lead to competing structures. An oligonucleotide system has been designed to demonstrate this competition. Systematic variation of its components give insight into the requirements for optimal binding of a third strand. A palindromic, homopyrimidine oligonucleotide of 22 bases was designed to form an overlapping 9-base, Watson-Crick (WC), duplex with a partly complementary 22-base purine-rich oligonucleotide. This leaves two free 3' extensions under conditions when only the duplex is stable. If the conditions, however, favour triplex formation the pyrimidine tail can compete with the purine-rich tail as third strand for the duplex forming Hoogsteen or reverse-Hoogsteen hydrogen bonding respectively with the purines in the WC double strand. The underlying triplexes and core duplex were synthesised and characterised as controls.en_ZA
dc.identifier.apacitationMills, M. G. (1998). <i>Competition between the purine and pyrimidine triple helix motifs in an oligonucleotide system</i>. (Thesis). University of Cape Town ,Faculty of Science ,Department of Molecular and Cell Biology. Retrieved from http://hdl.handle.net/11427/17946en_ZA
dc.identifier.chicagocitationMills, Martin George. <i>"Competition between the purine and pyrimidine triple helix motifs in an oligonucleotide system."</i> Thesis., University of Cape Town ,Faculty of Science ,Department of Molecular and Cell Biology, 1998. http://hdl.handle.net/11427/17946en_ZA
dc.identifier.citationMills, M. 1998. Competition between the purine and pyrimidine triple helix motifs in an oligonucleotide system. University of Cape Town.en_ZA
dc.identifier.ris TY - Thesis / Dissertation AU - Mills, Martin George AB - Triple helices are classified into two groups according to the composition and orientation of the third strand, namely the pyrimidine motif and the purine motif. These motifs constitute two separate fields of research. It was proposed, based on the alternative design rules, that the two motifs can in fact lead to competing structures. An oligonucleotide system has been designed to demonstrate this competition. Systematic variation of its components give insight into the requirements for optimal binding of a third strand. A palindromic, homopyrimidine oligonucleotide of 22 bases was designed to form an overlapping 9-base, Watson-Crick (WC), duplex with a partly complementary 22-base purine-rich oligonucleotide. This leaves two free 3' extensions under conditions when only the duplex is stable. If the conditions, however, favour triplex formation the pyrimidine tail can compete with the purine-rich tail as third strand for the duplex forming Hoogsteen or reverse-Hoogsteen hydrogen bonding respectively with the purines in the WC double strand. The underlying triplexes and core duplex were synthesised and characterised as controls. DA - 1998 DB - OpenUCT DP - University of Cape Town LK - https://open.uct.ac.za PB - University of Cape Town PY - 1998 T1 - Competition between the purine and pyrimidine triple helix motifs in an oligonucleotide system TI - Competition between the purine and pyrimidine triple helix motifs in an oligonucleotide system UR - http://hdl.handle.net/11427/17946 ER - en_ZA
dc.identifier.urihttp://hdl.handle.net/11427/17946
dc.identifier.vancouvercitationMills MG. Competition between the purine and pyrimidine triple helix motifs in an oligonucleotide system. [Thesis]. University of Cape Town ,Faculty of Science ,Department of Molecular and Cell Biology, 1998 [cited yyyy month dd]. Available from: http://hdl.handle.net/11427/17946en_ZA
dc.language.isoengen_ZA
dc.publisher.departmentDepartment of Molecular and Cell Biologyen_ZA
dc.publisher.facultyFaculty of Scienceen_ZA
dc.publisher.institutionUniversity of Cape Town
dc.subject.otherBiochemistryen_ZA
dc.titleCompetition between the purine and pyrimidine triple helix motifs in an oligonucleotide systemen_ZA
dc.typeMaster Thesis
dc.type.qualificationlevelMasters
dc.type.qualificationnameMScen_ZA
uct.type.filetypeText
uct.type.filetypeImage
uct.type.publicationResearchen_ZA
uct.type.resourceThesisen_ZA
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