Comparing high-throughput methods to measure antibody dependent cellular cytotoxicity during HIV infection

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dc.contributor.advisorPassmore, Jo-Annen_ZA
dc.contributor.advisorGamieldien, Hoyamen_ZA
dc.contributor.authorMbodo, Iyalooen_ZA
dc.date.accessioned2017-05-16T07:54:35Z
dc.date.available2017-05-16T07:54:35Z
dc.date.issued2014en_ZA
dc.description.abstractThe prevalence of HIV-1 is highest in Sub-Saharan Africa. Protective immune responses directed against HIV are complex and involve both cellular and humoral immunity. Based on the recent finding that the best correlate of protection against the first protective prophylactic RV144 vaccine were HIV-specific antibody responses, including those mediating natural killer (NK) cell antibody-dependent cellular cytotoxicity (ADCC), there has been considerable interest in measuring alternative roles for HIV-specific binding antibodies. The aim of this MSc dissertation was to optimise and compare two high-throughput flow cytometry based approaches - the GranToxilux and PanToxilux assays - to measure HIV-specific ADCC responses. To do this, NK cells from a panel of healthy HIV-negative individuals were screened for their ability to directly kill the tumour cell line K562, as a measure of direct NK cell cytotoxicity. The individual with the highest granzyme B and caspase activity against K562 cells was chosen as the universal NK cell donor for this study.en_ZA
dc.identifier.apacitationMbodo, I. (2014). <i>Comparing high-throughput methods to measure antibody dependent cellular cytotoxicity during HIV infection</i>. (Thesis). University of Cape Town ,Faculty of Health Sciences ,Division of Virology. Retrieved from http://hdl.handle.net/11427/24300en_ZA
dc.identifier.chicagocitationMbodo, Iyaloo. <i>"Comparing high-throughput methods to measure antibody dependent cellular cytotoxicity during HIV infection."</i> Thesis., University of Cape Town ,Faculty of Health Sciences ,Division of Virology, 2014. http://hdl.handle.net/11427/24300en_ZA
dc.identifier.citationMbodo, I. 2014. Comparing high-throughput methods to measure antibody dependent cellular cytotoxicity during HIV infection. University of Cape Town.en_ZA
dc.identifier.ris TY - Thesis / Dissertation AU - Mbodo, Iyaloo AB - The prevalence of HIV-1 is highest in Sub-Saharan Africa. Protective immune responses directed against HIV are complex and involve both cellular and humoral immunity. Based on the recent finding that the best correlate of protection against the first protective prophylactic RV144 vaccine were HIV-specific antibody responses, including those mediating natural killer (NK) cell antibody-dependent cellular cytotoxicity (ADCC), there has been considerable interest in measuring alternative roles for HIV-specific binding antibodies. The aim of this MSc dissertation was to optimise and compare two high-throughput flow cytometry based approaches - the GranToxilux and PanToxilux assays - to measure HIV-specific ADCC responses. To do this, NK cells from a panel of healthy HIV-negative individuals were screened for their ability to directly kill the tumour cell line K562, as a measure of direct NK cell cytotoxicity. The individual with the highest granzyme B and caspase activity against K562 cells was chosen as the universal NK cell donor for this study. DA - 2014 DB - OpenUCT DP - University of Cape Town LK - https://open.uct.ac.za PB - University of Cape Town PY - 2014 T1 - Comparing high-throughput methods to measure antibody dependent cellular cytotoxicity during HIV infection TI - Comparing high-throughput methods to measure antibody dependent cellular cytotoxicity during HIV infection UR - http://hdl.handle.net/11427/24300 ER - en_ZA
dc.identifier.urihttp://hdl.handle.net/11427/24300
dc.identifier.vancouvercitationMbodo I. Comparing high-throughput methods to measure antibody dependent cellular cytotoxicity during HIV infection. [Thesis]. University of Cape Town ,Faculty of Health Sciences ,Division of Virology, 2014 [cited yyyy month dd]. Available from: http://hdl.handle.net/11427/24300en_ZA
dc.language.isoengen_ZA
dc.publisher.departmentDivision of Virologyen_ZA
dc.publisher.facultyFaculty of Health Sciencesen_ZA
dc.publisher.institutionUniversity of Cape Town
dc.subject.otherVirologyen_ZA
dc.titleComparing high-throughput methods to measure antibody dependent cellular cytotoxicity during HIV infectionen_ZA
dc.typeMaster Thesis
dc.type.qualificationlevelMasters
dc.type.qualificationnameMSc (Med)en_ZA
uct.type.filetypeText
uct.type.filetypeImage
uct.type.publicationResearchen_ZA
uct.type.resourceThesisen_ZA
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