Investigating the role of B and T lymphocytes in the course of murine Leishmania Major infection

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dc.contributor.advisorBrombacher, Franken_ZA
dc.contributor.authorEinhorn, Andrewen_ZA
dc.date.accessioned2014-07-29T09:03:20Z
dc.date.available2014-07-29T09:03:20Z
dc.date.issued2013en_ZA
dc.descriptionIncludes abstract.
dc.descriptionIncludes bibliographical references.
dc.description.abstractThis thesis is composed of two parts. The first is an investigation of the role of B-effector cells in the course of murine Leishmania Major (L. major). The second is a bioinformatic analysis of microarray slides of activated CD4 T-cells from L. major infected BALB/c and C57BL/6 mice in order to investigate the genetic determinants of their divergent disease phenotypes. Overall this thesis is an investigation of the role of B-and T-lymphocytes during the course of L. major infection in mice. B-cells are not traditionally thought to play a role in the pathogenesis of Leishmania major infection. It is well documented that T-helper 1(Th1) and T-helper 2 (Th2) responses lead to resistance and susceptiblity to L. major respectively. Recent studies have now shown that B-cells are capable to producing key T-cell differentiating cytokines such as IL-4 and IFN-γ. More specifically, two new B-cell populations have been identified: B effector 1 (Be1) and B effector 2 (Be2) cells, which produce IFN-γ and IL-4 respectively. Using mice lacking the IL-4Rα on B-cells, and thus incapble of producing Be2 cells, we investigate the contribution of Be2 cells to BALB/c susceptibility. We confirm that the delection of the IL-4Rα on B-cells renders the previously susceptible BALB/c mice resistant to L. major strain LV39 and further confirm the phenotype using the more the virulent IL81 strain. We demonstrate that mice lacking Be2 cells exhibit a dimished Th2 response and an enhanced Th1 response, suggesting that B-cells, in addition to their role in antibody production, may also play a role in shaping T-helper responses in vivo. In the case of L.major, this provides evidence for a novel link between B cells and the cellular immune response.en_ZA
dc.identifier.apacitationEinhorn, A. (2013). <i>Investigating the role of B and T lymphocytes in the course of murine Leishmania Major infection</i>. (Thesis). University of Cape Town ,Faculty of Health Sciences ,Department of Medicine. Retrieved from http://hdl.handle.net/11427/3380en_ZA
dc.identifier.chicagocitationEinhorn, Andrew. <i>"Investigating the role of B and T lymphocytes in the course of murine Leishmania Major infection."</i> Thesis., University of Cape Town ,Faculty of Health Sciences ,Department of Medicine, 2013. http://hdl.handle.net/11427/3380en_ZA
dc.identifier.citationEinhorn, A. 2013. Investigating the role of B and T lymphocytes in the course of murine Leishmania Major infection. University of Cape Town.en_ZA
dc.identifier.ris TY - Thesis / Dissertation AU - Einhorn, Andrew AB - This thesis is composed of two parts. The first is an investigation of the role of B-effector cells in the course of murine Leishmania Major (L. major). The second is a bioinformatic analysis of microarray slides of activated CD4 T-cells from L. major infected BALB/c and C57BL/6 mice in order to investigate the genetic determinants of their divergent disease phenotypes. Overall this thesis is an investigation of the role of B-and T-lymphocytes during the course of L. major infection in mice. B-cells are not traditionally thought to play a role in the pathogenesis of Leishmania major infection. It is well documented that T-helper 1(Th1) and T-helper 2 (Th2) responses lead to resistance and susceptiblity to L. major respectively. Recent studies have now shown that B-cells are capable to producing key T-cell differentiating cytokines such as IL-4 and IFN-γ. More specifically, two new B-cell populations have been identified: B effector 1 (Be1) and B effector 2 (Be2) cells, which produce IFN-γ and IL-4 respectively. Using mice lacking the IL-4Rα on B-cells, and thus incapble of producing Be2 cells, we investigate the contribution of Be2 cells to BALB/c susceptibility. We confirm that the delection of the IL-4Rα on B-cells renders the previously susceptible BALB/c mice resistant to L. major strain LV39 and further confirm the phenotype using the more the virulent IL81 strain. We demonstrate that mice lacking Be2 cells exhibit a dimished Th2 response and an enhanced Th1 response, suggesting that B-cells, in addition to their role in antibody production, may also play a role in shaping T-helper responses in vivo. In the case of L.major, this provides evidence for a novel link between B cells and the cellular immune response. DA - 2013 DB - OpenUCT DP - University of Cape Town LK - https://open.uct.ac.za PB - University of Cape Town PY - 2013 T1 - Investigating the role of B and T lymphocytes in the course of murine Leishmania Major infection TI - Investigating the role of B and T lymphocytes in the course of murine Leishmania Major infection UR - http://hdl.handle.net/11427/3380 ER - en_ZA
dc.identifier.urihttp://hdl.handle.net/11427/3380
dc.identifier.vancouvercitationEinhorn A. Investigating the role of B and T lymphocytes in the course of murine Leishmania Major infection. [Thesis]. University of Cape Town ,Faculty of Health Sciences ,Department of Medicine, 2013 [cited yyyy month dd]. Available from: http://hdl.handle.net/11427/3380en_ZA
dc.language.isoengen_ZA
dc.publisher.departmentDepartment of Medicineen_ZA
dc.publisher.facultyFaculty of Health Sciencesen_ZA
dc.publisher.institutionUniversity of Cape Town
dc.subject.otherMedicineen_ZA
dc.titleInvestigating the role of B and T lymphocytes in the course of murine Leishmania Major infectionen_ZA
dc.typeMaster Thesis
dc.type.qualificationlevelMasters
dc.type.qualificationnameMScen_ZA
uct.type.filetypeText
uct.type.filetypeImage
uct.type.publicationResearchen_ZA
uct.type.resourceThesisen_ZA
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