Identification of Antigens Specific to Non-Tuberculous Mycobacteria: The Mce Family of Proteins as a Target of T Cell Immune Responses

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dc.contributor.authorCheckley, Anna M.en_ZA
dc.contributor.authorWyllie, David H.en_ZA
dc.contributor.authorScriba, Thomas J.en_ZA
dc.contributor.authorGolubchik, Tanyaen_ZA
dc.contributor.authorHill, Adrian V. S.en_ZA
dc.contributor.authorHanekom, Willem A.en_ZA
dc.contributor.authorMcShane, Helenen_ZA
dc.date.accessioned2015-11-09T13:22:18Z
dc.date.available2015-11-09T13:22:18Z
dc.date.issued2011en_ZA
dc.description.abstractThe lack of an effective TB vaccine hinders current efforts in combating the TB pandemic. One theory as to why BCG is less protective in tropical countries is that exposure to non-tuberculous mycobacteria (NTM) reduces BCG efficacy. There are currently several new TB vaccines in clinical trials, and NTM exposure may also be relevant in this context. NTM exposure cannot be accurately evaluated in the absence of specific antigens; those which are known to be present in NTM and absent from M. tuberculosis and BCG. We therefore used a bioinformatic pipeline to define proteins which are present in common NTM and absent from the M. tuberculosis complex, using protein BLAST, TBLASTN and a short sequence protein BLAST to ensure the specificity of this process. We then assessed immune responses to these proteins, in healthy South Africans and in patients from the United Kingdom and United States with documented exposure to NTM. Low level responses were detected to a cluster of proteins from the mammalian cell entry family, and to a cluster of hypothetical proteins, using ex vivo ELISpot and a 6 day proliferation assay. These early findings may provide a basis for characterising exposure to NTM at a population level, which has applications in the field of TB vaccine design as well as in the development of diagnostic tests.en_ZA
dc.identifier.apacitationCheckley, Anna M., Wyllie, David H., Scriba, Thomas J., Golubchik, T., Hill, Adrian V. S., Hanekom, Willem A., & McShane, H. (2011). Identification of Antigens Specific to Non-Tuberculous Mycobacteria: The Mce Family of Proteins as a Target of T Cell Immune Responses. <i>PLoS One</i>, http://hdl.handle.net/11427/14790en_ZA
dc.identifier.chicagocitationCheckley, Anna M., David H. Wyllie, Thomas J. Scriba, Tanya Golubchik, Adrian V. S. Hill, Willem A. Hanekom, and Helen McShane "Identification of Antigens Specific to Non-Tuberculous Mycobacteria: The Mce Family of Proteins as a Target of T Cell Immune Responses." <i>PLoS One</i> (2011) http://hdl.handle.net/11427/14790en_ZA
dc.identifier.citationCheckley, A. M., Wyllie, D. H., Scriba, T. J., Golubchik, T., Hill, A. V., Hanekom, W. A., & McShane, H. (2011). Identification of antigens specific to nontuberculous mycobacteria: the Mce family of proteins as a target of T cell immune responses. PloS one, 6(10), e26434. doi:10.1371/journal.pone.0026434en_ZA
dc.identifier.ris TY - Journal Article AU - Checkley, Anna M. AU - Wyllie, David H. AU - Scriba, Thomas J. AU - Golubchik, Tanya AU - Hill, Adrian V. S. AU - Hanekom, Willem A. AU - McShane, Helen AB - The lack of an effective TB vaccine hinders current efforts in combating the TB pandemic. One theory as to why BCG is less protective in tropical countries is that exposure to non-tuberculous mycobacteria (NTM) reduces BCG efficacy. There are currently several new TB vaccines in clinical trials, and NTM exposure may also be relevant in this context. NTM exposure cannot be accurately evaluated in the absence of specific antigens; those which are known to be present in NTM and absent from M. tuberculosis and BCG. We therefore used a bioinformatic pipeline to define proteins which are present in common NTM and absent from the M. tuberculosis complex, using protein BLAST, TBLASTN and a short sequence protein BLAST to ensure the specificity of this process. We then assessed immune responses to these proteins, in healthy South Africans and in patients from the United Kingdom and United States with documented exposure to NTM. Low level responses were detected to a cluster of proteins from the mammalian cell entry family, and to a cluster of hypothetical proteins, using ex vivo ELISpot and a 6 day proliferation assay. These early findings may provide a basis for characterising exposure to NTM at a population level, which has applications in the field of TB vaccine design as well as in the development of diagnostic tests. DA - 2011 DB - OpenUCT DO - 10.1371/journal.pone.0026434 DP - University of Cape Town J1 - PLoS One LK - https://open.uct.ac.za PB - University of Cape Town PY - 2011 T1 - Identification of Antigens Specific to Non-Tuberculous Mycobacteria: The Mce Family of Proteins as a Target of T Cell Immune Responses TI - Identification of Antigens Specific to Non-Tuberculous Mycobacteria: The Mce Family of Proteins as a Target of T Cell Immune Responses UR - http://hdl.handle.net/11427/14790 ER - en_ZA
dc.identifier.urihttp://hdl.handle.net/11427/14790
dc.identifier.urihttp://dx.doi.org/10.1371/journal.pone.0026434
dc.identifier.vancouvercitationCheckley Anna M, Wyllie David H, Scriba Thomas J, Golubchik T, Hill Adrian V S, Hanekom Willem A, et al. Identification of Antigens Specific to Non-Tuberculous Mycobacteria: The Mce Family of Proteins as a Target of T Cell Immune Responses. PLoS One. 2011; http://hdl.handle.net/11427/14790.en_ZA
dc.language.isoengen_ZA
dc.publisherPublic Library of Scienceen_ZA
dc.publisher.departmentDepartment of Paediatrics and Child Healthen_ZA
dc.publisher.facultyFaculty of Health Sciencesen_ZA
dc.publisher.institutionUniversity of Cape Town
dc.rightsThis is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.en_ZA
dc.rights.holder© 2011 Checkley et alen_ZA
dc.rights.urihttp://creativecommons.org/licenses/by/4.0en_ZA
dc.sourcePLoS Oneen_ZA
dc.source.urihttp://journals.plos.org/plosoneen_ZA
dc.subject.otherMycobacterium tuberculosisen_ZA
dc.subject.otherMycobacterium avium complexen_ZA
dc.subject.otherMycobacterium ulceransen_ZA
dc.subject.otherEnzyme-linked immunoassaysen_ZA
dc.subject.otherTuberculosisen_ZA
dc.subject.otherBLAST algorithmen_ZA
dc.subject.otherImmune responseen_ZA
dc.subject.otherT cellsen_ZA
dc.titleIdentification of Antigens Specific to Non-Tuberculous Mycobacteria: The Mce Family of Proteins as a Target of T Cell Immune Responsesen_ZA
dc.typeJournal Articleen_ZA
uct.type.filetypeText
uct.type.filetypeImage
uct.type.publicationResearchen_ZA
uct.type.resourceArticleen_ZA
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