The cloning and characterization of a Butyrivibrio fibrisolvens H17c glnA regulatory element in E. coli

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dc.contributor.advisorAbratt, Valerie Roseen_ZA
dc.contributor.authorSamsodien, Anwaren_ZA
dc.date.accessioned2016-05-16T11:58:40Z
dc.date.available2016-05-16T11:58:40Z
dc.date.issued1997en_ZA
dc.descriptionBibliography: pages 119-137.en_ZA
dc.description.abstractButyrivibrio fibrisolvens HI 7C is an important anaerobic bacterium which occurs in the rumen of most ruminants. A key factor affecting the growth of this bacterium is the availability of nitrogen sources, particularly in the form of ammonia. The aims of this study were to attempt the isolation of a gene/genes involved in the regulation of the B.fibrisolvens type III glutamine synthetase (GS), which is a key enzyme involved in ammonia assimilation in rumen bacteria. An existing B. fibrisolvens gene bank, as well as the B. fibrisolvens glnA gene cloned onto a low 11 copy number plasmid, were used to generate a heterologous, Escherichia coli- based, two plasmid, in trans system. This system was used to isolate an E. coli clone which showed increased GS activity levels (2.5-fold) and a retarded growth rate phenotype in complete media.en_ZA
dc.identifier.apacitationSamsodien, A. (1997). <i>The cloning and characterization of a Butyrivibrio fibrisolvens H17c glnA regulatory element in E. coli</i>. (Thesis). University of Cape Town ,Faculty of Science ,Department of Molecular and Cell Biology. Retrieved from http://hdl.handle.net/11427/19682en_ZA
dc.identifier.chicagocitationSamsodien, Anwar. <i>"The cloning and characterization of a Butyrivibrio fibrisolvens H17c glnA regulatory element in E. coli."</i> Thesis., University of Cape Town ,Faculty of Science ,Department of Molecular and Cell Biology, 1997. http://hdl.handle.net/11427/19682en_ZA
dc.identifier.citationSamsodien, A. 1997. The cloning and characterization of a Butyrivibrio fibrisolvens H17c glnA regulatory element in E. coli. University of Cape Town.en_ZA
dc.identifier.ris TY - Thesis / Dissertation AU - Samsodien, Anwar AB - Butyrivibrio fibrisolvens HI 7C is an important anaerobic bacterium which occurs in the rumen of most ruminants. A key factor affecting the growth of this bacterium is the availability of nitrogen sources, particularly in the form of ammonia. The aims of this study were to attempt the isolation of a gene/genes involved in the regulation of the B.fibrisolvens type III glutamine synthetase (GS), which is a key enzyme involved in ammonia assimilation in rumen bacteria. An existing B. fibrisolvens gene bank, as well as the B. fibrisolvens glnA gene cloned onto a low 11 copy number plasmid, were used to generate a heterologous, Escherichia coli- based, two plasmid, in trans system. This system was used to isolate an E. coli clone which showed increased GS activity levels (2.5-fold) and a retarded growth rate phenotype in complete media. DA - 1997 DB - OpenUCT DP - University of Cape Town LK - https://open.uct.ac.za PB - University of Cape Town PY - 1997 T1 - The cloning and characterization of a Butyrivibrio fibrisolvens H17c glnA regulatory element in E. coli TI - The cloning and characterization of a Butyrivibrio fibrisolvens H17c glnA regulatory element in E. coli UR - http://hdl.handle.net/11427/19682 ER - en_ZA
dc.identifier.urihttp://hdl.handle.net/11427/19682
dc.identifier.vancouvercitationSamsodien A. The cloning and characterization of a Butyrivibrio fibrisolvens H17c glnA regulatory element in E. coli. [Thesis]. University of Cape Town ,Faculty of Science ,Department of Molecular and Cell Biology, 1997 [cited yyyy month dd]. Available from: http://hdl.handle.net/11427/19682en_ZA
dc.language.isoengen_ZA
dc.publisher.departmentDepartment of Molecular and Cell Biologyen_ZA
dc.publisher.facultyFaculty of Scienceen_ZA
dc.publisher.institutionUniversity of Cape Town
dc.subject.otherMolecular and Cell Biologyen_ZA
dc.titleThe cloning and characterization of a Butyrivibrio fibrisolvens H17c glnA regulatory element in E. colien_ZA
dc.typeMaster Thesis
dc.type.qualificationlevelMasters
dc.type.qualificationnameMScen_ZA
uct.type.filetypeText
uct.type.filetypeImage
uct.type.publicationResearchen_ZA
uct.type.resourceThesisen_ZA
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